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Journal of Assisted Reproduction and Genetics

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01-06-2012 | Fertility Preservation

In-vitro maturation of human oocytes: before or after vitrification?

Authors: Giovanna Fasano, Isabelle Demeestere, Yvon Englert

Published in: Journal of Assisted Reproduction and Genetics | Issue 6/2012

Abstract

Purpose

This study aims to determine if in-vitro maturation (IVM) of human immature oocytes should be performed before or after vitrification.

Methods

A total of 184 immature oocytes were randomly divided into two different groups: 100 were vitrified at metaphase II (MII) stage 24 h-48 h after IVM (group 1) and 84 were immediately vitrified at germinal vesicle (GV) or metaphase I (MI) stages and in vitro matured after warming (group 2).

Results

Survival rate after warming was similar in both groups (86.9% versus 84.5%). However, oocyte maturation rate per collected oocyte was significantly higher for oocytes matured before vitrification (group 1, 46%) than for oocytes vitrified before IVM (group 2, 23.8%) (p < 0.01). Consequently, the number of MII oocytes inseminated per oocyte collected was significantly higher for group 1 (40%) than for group 2 (23.8%) (p < 0.05).

Conclusion

IVM procedure is more efficient when it is performed before oocyte vitrification.

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Title: In-vitro maturation of human oocytes: before or after vitrification?
Authors: Giovanna Fasano
Isabelle Demeestere
Yvon Englert
Publication date: 01-06-2012
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 6/2012
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-012-9751-9

At a glance: The STEP trials

Springer Medicine

In-vitro maturation of human oocytes: before or after vitrification?

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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