Top

Journal of Assisted Reproduction and Genetics

Published in:

01-06-2011 | Gamete Biology

Differentially expressed micoRNAs in human oocytes

Authors: Yan-Wen Xu, Bin Wang, Chen-Hui Ding, Tao Li, Fang Gu, Canquan Zhou

Published in: Journal of Assisted Reproduction and Genetics | Issue 6/2011

Abstract

Purpose

To identify differentially expressed microRNAs (miRNAs) and expression patterns of specific miRNAs during meiosis in human oocytes.

Materials and methods

To identify differentially expressed miRNAs, GV oocytes and MII oocytes matured at conventional FSH levels (5.5 ng/ml) were analyzed by miRNA microarray. Real-time RT-PCR was used to confirm the changed miRNAs. To validate the dynamic changes of miRNAs from GV to MII stages, oocytes were divided into four groups (#1–4), corresponding to GV oocytes, MI oocytes, MII oocytes matured in conventional FSH level and MII oocytes matured in high FSH level (2,000 ng/ml) respectively.

Results

Compared with GV oocytes, MII oocytes exhibited up-regulation of 4 miRNAs (hsa-miR-193a-5p, hsa-miR-297, hsa-miR-625 and hsa-miR-602), and down-regulation of 11 miRNAs (hsa-miR-888*, hsa-miR-212, hsa-miR-662, hsa-miR-299-5p, hsa-miR-339-5p, hsa-miR-20a, hsa-miR-486-5p, hsa-miR-141*, hsa-miR-768-5p, hsa-miR-376a and hsa-miR-15a). RT-PCR analysis of hsa-miR-15a and hsa-miR-20a expression revealed concordant dynamic changes in oocytes from group 1 to group 4.

Conclusion(s)

Specific miRNAs in human oocytes had dynamic changes during meiosis. High-concentration FSH in IVM medium led to reverse effect on the expression of hsa-miR-15a and hsa-miR-20a.

Paynton BV, Rempel R, Bachvarova R. Changes in state of adenylation and time course of degradation of maternal mRNAs during oocyte maturation and early embryonic development in the mouse. Dev Biol. 1988;129:304–14.PubMedCrossRef

Su YQ, Sugiura K, Woo Y, Wiggleworth K, Kamdar S, Affourtit J, et al. Selective degradation of transcripts during meiotic maturation of mouse oocytes. Dev Biol. 2007;302(1):104–17.PubMedCrossRef

Cui XS, Li XY, Yin XJ, Kong IK, Kang JJ, Kim NH. Maternal gene transcription in mouse oocytes genes implicated in oocyte maturation and fertilization. J Reprod Dev. 2007;53(2):405–18.PubMedCrossRef

Mamo S, Carter F, Lonergan P, Leal C, Naib A, McGettigan P, et al. Sequential analysis of global gene expression profiles in immature and in vitro matured bovine oocytes: potential molecular markers of oocyte maturation. BMC Genomics. 2011;12:151.PubMedCrossRef

Assou S, Anahory T, Pantesco V, Le Carrour T, Pellestor F, Klein B, et al. The human cumulus–oocyte complex gene-expression profile. Hum Reprod. 2006;21(7):1705–19.PubMedCrossRef

Bartel DP. MicroRNAs: fenomics, biogenesis, mechanism, and function. Cell. 2004;116:281–97.PubMedCrossRef

Lewis BP, Burge CB, Bartel DP. Conserved seed pairing, often flanked by adenosines, indicates that thousands of human genes are microRNA targets. Cell. 2005;120:15–20.PubMedCrossRef

Tang F, Kaneda M, O’Carroll D, Hajkova P, Barton S, Sun YA, et al. Maternal microRNAs are essential for mouse zygotic development. Genes Dev. 2007;21(6):644–8.PubMedCrossRef

Murchison EP, Stein P, Xuan Z, Pan H, Zhang MQ, Schultz RM, et al. Critical roles for Dicer in the female germline. Genes Dev. 2007;21(6):682–93.PubMedCrossRef

10.

Svoboda P, Stein P, Hayashi H, Schultz RM. Selective reduction of dormant maternal mRNAs in mouse oocytes by RNA interference. Development. 2000;127:4147–56.PubMed

11.

Liu HC, Tang YX, He ZY, Rosenwaks Z. Dicer is a key player in oocyte maturation. J Assist Reprod Genet. 2010;27:571–850.PubMedCrossRef

12.

Suh N, Baehner L, Moltzahn F, Melton C, Shenoy A, Chen J, et al. MicroRNA function is globally suppressed in mouse oocytes and early embryos. Curr Biol. 2010;20:271–7.PubMedCrossRef

13.

Xu YW, Peng YT, Wang B, Zeng YH, Zhuang GL, Zhou CQ. High follicle-stimulating hormone increases aneuploidy in human oocytes matured in vitro. Fertil & Steril. 2011;95(1):99–104.CrossRef

14.

Ro S, Park C, Sanders KM, McCarrey JR, Yan W. Cloning and expression profiling of testis-expressed microRNAs. Dev Biol. 2007;311:592–602.PubMedCrossRef

15.

Schultz RM, Davis Jr W, Stein P, Svoboda P. Reprogramming of gene expression during preimplantation development. J Exp Zool. 1999;285(3):276–82.PubMedCrossRef

16.

Tesarik J, Kopecny V, Plachot M, Mandelbaum J. Early morphological signs of embryonic genome expression in human preimplantation development as revealed by quantitative electron microscopy. Dev Biol. 1988;128:15–20.PubMedCrossRef

17.

Ma J, Flemr M, Stein P, Berninger P, Malik R, Zavolan M, et al. MicroRNA activity is suppressed in mouse oocytes. Curr Biol. 2010;20:265–70.PubMedCrossRef

18.

Stáhlerg A, Bengtsson M, Hemberg M, Semb H. Quantitative Transcription factor analysis of undifferentiated single human embryonic stem cells. Clin Chem. 2009;55(12):2162–70.CrossRef

19.

Stáhlerg A, Bengtsson M. Single-cell gene expression profiling using reverse transcription quantitative real-time PCR. Methods. 2010;50:282–8.CrossRef

20.

Mtango NR, Potireddy S, Latham KE. Expression of microRNA processing machinery genes in rhesus monkey oocytes and embryos of different developmental potentials. Mol Reprod Dev. 2009;76(3):255–69.PubMedCrossRef

21.

Linsley PS, Schelter J, Burchard J, Kibukawa M, Martin MM, Bartz SR, et al. Transcripts targeted by the microRNA-16 family cooperatively regulate cell cycle progression. Mol Cell Biol. 2007;27(6):2240–52.PubMedCrossRef

22.

Cimmino A, Calin GA, Fabbri M, Lorio MV, Ferracin M, Shimizu M, et al. miR-15 and miR-16 induce apoptosis by targeting BCL2. Proc Natl Acad Sci U S A. 2005;102(39):13944–9.PubMedCrossRef

23.

Yoon SJ, Kim EY, Kim YS. Role of Bcl2-like 10 (Bcl2l10) in regulating mouse oocyte maturation. Biol Reprod. 2009;81(3):497–506.PubMedCrossRef

24.

Guillemin Y, Lalle P, Gillet G. Oocytes and early embryos selectively express the survival factor BCL2L10. J Mol Med. 2009;87:923–40.PubMedCrossRef

25.

Isoda M, Kanemori Y, Nakajo N, Uchida S, Yamashita K, Ueno H, et al. The extracellular signal-regulated kinase-mitogen-activated protein kinase pathway phosphorylates and targets Cdc25A for SCF^β-TrCP-dependent degradation for cell cycle arrest. Mol Biol Cell. 2009;20(20):2186–95.PubMedCrossRef

26.

Solc P, Saskova A, Baran V, Kubelka M, Schultz RM, Motlik J. CDC25A phosphatase controls meiosis I progression in mouse oocytes. Dev Biol. 2008;317(1):260–9.PubMedCrossRef

27.

Dursun P, Gultekin M, Yuce K, Ayhan A. What is the underlying cause of aneuploidy associated with increasing maternal age? Is it associated with elevated levels of gnoadotropins? Medical Hypotheses. 2006;66:143–7.PubMedCrossRef

28.

Eichenlaub-Ritter U, Winterscheidt U, Vogt E, Shen Y, Tinneberg H, Sorensen R. 2-Methoxyestradiol induces spindle aberrations, chromosome congression failure, and nondisjunction in mouse oocytes. Biol Reprod. 2007;76:784–93.PubMedCrossRef

29.

Yin H, Baart E, Betzendahl I, Eichenlaub-Ritter U. Diazepam induces meiotic delay, aneuploidy and predivision of homologues and chromatids in mammalian oocytes. Mutagenesis. 1998;13:567–80.PubMedCrossRef

30.

Can A, Semiz O. Diethylstilbestrol (DES)-induced cell cycle delay and meiotic spindle disruption in mouse oocytes during in-vitro maturation. Mol Hum Reprod. 2000;6:154–62.PubMedCrossRef

31.

Wakefield JG, Stephens DJ, Tavare JM. A role for glycogen synthase kinase-3 in mitotic spindle dynamics and chromosome alignment. J Cell Sci. 2003;116:637–46.PubMedCrossRef

32.

Wang X, Liu XT, Dunn R, Ohl DA, Smith GD. Glycogen synthase kinase-3 regulates mouse oocyte homologue segregation. Mol Reprod Dev. 2003;64:96–105.PubMedCrossRef

Title: Differentially expressed micoRNAs in human oocytes
Authors: Yan-Wen Xu
Bin Wang
Chen-Hui Ding
Tao Li
Fang Gu
Canquan Zhou
Publication date: 01-06-2011
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 6/2011
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9590-0

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Differentially expressed micoRNAs in human oocytes

Abstract

Purpose

Materials and methods

Results

Conclusion(s)

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Materials and methods

Results

Conclusion(s)

Please log in to get access to this content

Other articles of this Issue 6/2011

IVM is an alternative for patients with PCO after failed conventional IVF attempt

In vitro maturation of ovine oocytes using different maturation media: effect of human menopausal serum

The impact of obesity on egg quality

Follicle environment and quality of in vitro matured oocytes

Health of IVM children

Anti-mullerian hormone as a predictive marker for the selection of women for oocyte in vitro maturation treatment