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Journal of Assisted Reproduction and Genetics

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01-06-2011 | Gamete Biology

Correlation of oocyte morphometry parameters with woman’s age

Authors: Claudia Valeri, Sebastiana Pappalardo, Massimo De Felici, Claudio Manna

Published in: Journal of Assisted Reproduction and Genetics | Issue 6/2011

Abstract

Purpose

Aim of this study was to evaluate morphometric parameters of metaphase II oocytes, including cytoplasm diameter (CD), zona pellucida thickness (ZPT) and width of the perivitelline space (PS), in relation with zona pellucida birefringence, spindle presence and age of the woman.

Methods

Oocytes were classified into groups according to zona birefringence (low or high zona birefringence, LZB and HZB, respectively) and presence or absence of a visible spindle (SP and aSP, respectively).

Results

HZB oocytes showed a thicker zona (17.7 ± 0.3 μm) than LZB oocytes (16.7 ± 0.3 μm, p < 0.01). Moreover, PS was narrower in HZB and SP oocytes than in LZB (p < 0,001) and aSP (p < 0,05) oocytes. Finally, we found that CD and ZPT linearly decrease with age of the woman (CD r = 0.028: p < 0.01; ZPT r = 0.050: p < 0.0001).

Conclusion

Our results evidence an association in human oocytes between zona pellucida and spindle birefringence and defined morphometric parameters and a decrease of oocyte size and ZPT as a function of women’s age.

Serhal PF, Ranieri DM, Kinis A, Marchant S, Davies M, Khadum IM. Oocyte morphology predicts outcome of intracytoplasmic sperm injection. Hum Reprod. 1997;12:1267–70.PubMedCrossRef

Rienzi L, Ubaldi FM, Iacobelli M, Minasi MG, Romano S, Ferrero S, et al. Significance of metaphase II human oocyte morphology on ICSI outcome. Fertil Steril. 2008;90:1692–700.PubMedCrossRef

Xia P. Intracytoplasmic sperm injection: correlation of oocyte grade based on polar body, perivitelline space and cytoplasmic inclusions with fertilization rate and embryo quality. Hum Reprod. 1997;12(8):1750–5.PubMedCrossRef

Ebner T, Yaman C, Moser M, Sommergruber M, Feichtinger O, Tews G. Prognostic value of first polar body morphology on fertilization rate and embryo quality in intracytoplasmic sperm injection. Hum Reprod. 2000;15(2):427–30.PubMedCrossRef

Balaban B, Urman B, Sertac A, Alatas C, Aksoy S, Mercan R. Oocyte morphology does not affect fertilization rate, embryo quality and implantation rate after intracytoplasmic sperm injection. Hum Reprod. 1998;13(12):3431–3.PubMedCrossRef

Baczkowski T, Kurzawa R, Głabowski W. Methods of embryo scoring in in vitro fertilization. Reprod Biol. 2004;4(1):5–22.PubMed

Tao T, Zhang W, Del Valle A. Human oocyte cryopreservation. Curr Opin Obstet Gynecol. 2009;21(3):247–52.PubMedCrossRef

De Santis L, Cino I, Coticchio G, Fusi FM, Papaleo E, Rabellotti E, et al. Objective evaluation of the viability of cryopreserved oocytes. Reprod Biomed Online. 2007;15(3):338–45.PubMedCrossRef

Verlinsky Y, Ginsberg N, Lifchez A, Valle J, Moise J, Strom CM. Analysis of the first polar body: preconception genetic diagnosis. Hum Reprod. 1990;5(7):826–9.PubMed

10.

Patrizio P, Fragouli E, Bianchi V, Borini A, Wells D. Molecular methods for selection of the ideal oocyte. Reprod Biomed Online. 2007;15:346–53.PubMedCrossRef

11.

Oldenbourg R. A new view on polarized microscopy. Nature. 1996;381:175–2008.CrossRef

12.

Wang WH, Meng L, Hackett RJ, Keefe DL. Developmental ability of human oocytes with or without birefringent spindles imaged by Polscope before insemination. Hum Reprod. 2001;16(7):1464–8.PubMedCrossRef

13.

Rama Raju GA, Prakash GJ, Krishna KM, Madan K. Meiotic spindle and zona pellucida characteristics as predictors of embryonic development: a preliminary study using PolScope imaging. Reprod Biomed Online. 2007;14(2):166–74.PubMedCrossRef

14.

Montag M, Schimming T, Koster M, Zhou C, Dorn C, Rosing B, et al. Oocyte zona birefringence intensity is associated with embryonic implantation potential in ICSI cycles. Reprod Biomed Online. 2007;16:239–44.CrossRef

15.

Madaschi C, Aoki T, Paes de Almeida Ferreira Braga D, de Cassia Savio Figueira R, Semiao Francisco L, Iaconelli AJ, et al. Zona pellucida birefringence score and meiotic spindle visualization in relation to embryo development and ICSI outcomes. Reprod Biomed Online. 2009;18:681–6.PubMedCrossRef

16.

Manna C, Patrizi G, Rahmanm A, Sallam H. Experimental results on the recognition of embryos in human assisted reproduction. Reprod Biomed Online. 2004;8:460–9.PubMedCrossRef

17.

Keefe D, Tran P, Pellegrini C, Oldenbourg R. Polarized light microscopy and digital image processing identify a multilaminar structure of the hamster zona pellucida. Hum Reprod. 1997;12:1250–2.PubMedCrossRef

18.

Montag M, van der Ven H. Oocyte assessment and embryo viability prediction: birefringence imaging. Reprod Biomed Online. 2008;17:454–60.PubMedCrossRef

19.

Gomes LM, Canha Ados S, Dzik A, Novo NF, Juliano Y, Dos Santos SI, et al. The age as a predictive factor in in vitro fertilization cycles. Rev Bras Ginecol Obstet. 2009;31(5):230–4.PubMedCrossRef

20.

Burger HG. Diagnostic role of follicle-stimulating hormone (FSH) measurements during the menopausal transition-an analysis of FSH, oestradiol and inhibin. Eur J Endocrinol. 1994;130:38–42.PubMedCrossRef

21.

El-Toukhy T, Khalaf Y, Hart R, Taylor A, Braude P. Young age does not protect against the adverse effects of reduced ovarian reserve-an eight year study. Hum Reprod. 2002;17(6):1519–24.PubMedCrossRef

22.

Montag M, Schimming T, van der Ven H. Spindle imaging in human oocytes: the impact of the meiotic cell cycle. Reprod Biomed Online. 2006;12(4):442–6.PubMedCrossRef

23.

Shen Y, Stalf T, Mehnert C, Eichenlaub-Ritter U, Tinneberg HR. High magnitude of light retardation by the zona pellucida is associated with conception cycles. Hum Reprod. 2005;20(6):1596–606.PubMedCrossRef

24.

Ebner T, Balaban B, Moser M, Shebl O, Urman B, Ata B, et al. Automatic user-independent zona pellucida imaging at the oocyte stage allows for the prediction of preimplantation. Fertil Steril. 2010;94(3):913–20.PubMedCrossRef

25.

Pelletier C, Keefe D, Trimarchi JR. Non invasive polarized light microscopy quantitatively distinguished the multilaminar structure of the zona pellucid of living human eggs and embryo. Fertil Steril. 2004;81:850–6.PubMedCrossRef

26.

Kilani SS, Cooke S, Kan AK, Chapman MG. Do age and extended culture affect the architecture of the zona pellucida of human oocytes and embryos? Zygote. 2006;14(1):39–44.PubMedCrossRef

27.

Cohen J, Inge KL, Suzman M, Wider SR, Wright GR. Videocinematography of fresh and cryopreserved embryos: a retrospective analysis of embryonic morphology and implantation. Fertil Steril. 1989;51:820–7.PubMed

28.

Garside WT, de Mola JR Loret, Bucci JA, Tureck RW, Heyner S. Sequential analysis of zona thickness during in vitro culture of human zygotes: correlation with embryo quality, age, and implantation. Mol Reprod Dev. 1997;47(1):99–104.PubMedCrossRef

29.

Miao YL, Kikuchi K, Sun QY, Schatten H. Oocyte aging: cellular and molecular changes, developmental potential and reversal possibility. Hum Reprod Update. 2009;15:273–85.CrossRef

30.

Nawroth F, Müller P, Wolf C, Sudik R. Is the Zona pellucida thickness of metaphase-II oocytes in an IVF/ICSI program influenced by the patient’s age? Gynecol Obstet Investig. 2001;52(1):55–9.CrossRef

31.

Wassarman PM, Litscher ES. Mammalian fertilization: the egg's multifunctional zona pellucida. Int J Dev Biol. 2008;52(5–6):65–76.

32.

Rankin T, Talbot P, Lee E, Dean J. Abnormal zonae pellucidae in mice lacking ZP1 result in early embryonic loss. Development. 1999;126:3847–55.PubMed

33.

Chernoff R. Protein and older adults. J Am Coll Nutr. 2004;23(6):627S–30S.PubMed

34.

Caprari P, Scuteri A, Salvati AM, Bauco C, Cantafora A, Masella R, et al. Aging and red blood cell membrane: a study of centenarians. Exp Gerontol. 1999;34(1):47–57.PubMedCrossRef

35.

Chen H, Hardy MP, Huhtaniemi I, Zirkin BR. Age-related decreased Leydig cell testosterone production in the brown Norway rat. J Androl. 1994;15(6):551–7.PubMed

36.

Schliess F, Reinehr R, Häussinger D. Osmosensing and signaling in the regulation of mammalian cell function. FEBS J. 2007;274(22):5799–803.PubMedCrossRef

37.

Agarwal A, Gupta S, Sharma RK. Role of oxidative stress in female reproduction. Reprod Biol Endocrinol. 2005;14:3–28.

38.

Andux S, Ellis RE. Apoptosis maintains oocytes quality in aging Caenorhabditis elegans females. PLoS Genet. 2008;4(12):e1000295.PubMedCrossRef

Title: Correlation of oocyte morphometry parameters with woman’s age
Authors: Claudia Valeri
Sebastiana Pappalardo
Massimo De Felici
Claudio Manna
Publication date: 01-06-2011
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 6/2011
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-011-9555-3

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Correlation of oocyte morphometry parameters with woman’s age

Abstract

Purpose

Methods

Results

Conclusion

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Abstract

Purpose

Methods

Results

Conclusion

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