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Journal of Assisted Reproduction and Genetics

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01-11-2010 | Technical Innovations

The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage

Authors: Tyl H. Taylor, Janice W. Gilchrist, Susan V. Hallowell, Kelly K. Hanshew, John J. Orris, Michael J. Glassner, J. David Wininger

Published in: Journal of Assisted Reproduction and Genetics | Issue 11/2010

Abstract

Purpose

A laser is commonly used to remove a blastomere from an embryo for genetic testing. The laser uses intense heat which could possibly disrupt embryo development. It is the goal of this study to test the effects of different laser pulse lengths (and consequently heat) on the embryo biopsy procedure and embryo development.

Methods

Each embryo biopsy was performed randomly utilizing laser pulse lengths of 0.604mS (group I), 0.708mS (group II), and 1.010mS (group III).

Results

For groups I, II, and III, 83, 86, and 71 embryos were biopsied, respectively. There was no difference in day 5 embryo quality or lysed blastomeres between groups. Average number of blastomeres biopsied between group I (1.0 ± 0.0), II (1.0 ± 0.2), and III (1.1 ± 0.2) was significant (0.0001).

Conclusion

Our data demonstrates that laser pulse length does not influence the embryo biopsy procedure or embryo development.

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Title: The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage
Authors: Tyl H. Taylor
Janice W. Gilchrist
Susan V. Hallowell
Kelly K. Hanshew
John J. Orris
Michael J. Glassner
J. David Wininger
Publication date: 01-11-2010
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 11/2010
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-010-9461-0

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

The effects of different laser pulse lengths on the embryo biopsy procedure and embryo development to the blastocyst stage

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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