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Journal of Assisted Reproduction and Genetics
Published in: Journal of Assisted Reproduction and Genetics 5/2010

01-05-2010 | Fertility Preservation

In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes

Authors: Bita Ebrahimi, Mojtaba Rezazadeh Valojerdi, Poopak Eftekhari-Yazdi, Hossein Baharvand

Published in: Journal of Assisted Reproduction and Genetics | Issue 5/2010

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Abstract

Purpose

The purpose of this study was to evaluate the effects of cryotop vitrification of sheep cumulus-oocyte complexes (COCs) on oocyte maturation, apoptotic gene expression and incidence of chromosomal abnormalities.

Methods

Freshly isolated (control group) and vitrified-warmed COCs (cryotop group) were matured in vitro. The expression of pro- and anti-apoptotic genes was investigated by real-time PCR. The incidence of numerical chromosomal abnormalities was evaluated by cytogenetic analysis.

Results

The mean percentage of oocytes in the cryotop and control groups that reached metaphase II was 49.25 ± 3.01% and 51.94 ± 2.7% respectively. The expression rates of pro- and anti-apoptotic genes were similar in both groups, whereas the incidence of numerical chromosomal abnormalities was higher in the cryotop group compared to the control group (42.5% vs. 20%, p < 0.05).

Conclusion

Although cryotop vitrification of COCs did not affect the incidence of oocyte maturation or apoptotic gene expression, significant deficiencies in the maintenance of oocyte chromosomal organization were seen.
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Metadata
Title
In vitro maturation, apoptotic gene expression and incidence of numerical chromosomal abnormalities following cryotop vitrification of sheep cumulus-oocyte complexes
Authors
Bita Ebrahimi
Mojtaba Rezazadeh Valojerdi
Poopak Eftekhari-Yazdi
Hossein Baharvand
Publication date
01-05-2010
Publisher
Springer US
Published in
Journal of Assisted Reproduction and Genetics / Issue 5/2010
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI
https://doi.org/10.1007/s10815-010-9401-z

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