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Journal of Assisted Reproduction and Genetics

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01-12-2009 | ANDROLOGY

Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion

Authors: Talieh Kazerooni, Nasrin Asadi, Leila Jadid, Marjaneh Kazerooni, Alireza Ghanadi, Fariborz Ghaffarpasand, Yasaman Kazerooni, Jaleh Zolghadr

Published in: Journal of Assisted Reproduction and Genetics | Issue 11-12/2009

Abstract

Objective

To evaluate the sperm’s chromatin quality in couples with spontaneous recurrent abortion.

Methods

Thirty couples with spontaneous recurrent abortion (case group) and 30 fertile couples (control group) referring to Zeinabieh Gynecology clinic of Shiraz were included. Semen samples were collected for each participant and were used for standard semen analysis and sperm nuclear maturity tests including Chromomycin A3 (CMA3), Aniline Blue (AB) staining and Acridine Orange (AO) test (by light microscopy).

Result

Patients in case group had significantly higher percentage of CMA3 (p < 0.001) and AB (p < 0.001) positive spermatozoa compared to controls. However AO results did not differ significantly between groups (p = 0.656). Sperm morphology and progressive motility were negatively correlated with CMA3 (p = 0.001 and p = 0.043) and AB (p = 0.015 and p = 0.031) respectively.

Conclusion

Evaluation of the sperm’s quality via CMA3 and AB staining could be considered as one of the complementary tests of semen analysis for assessment of male factor in couples with spontaneous recurrent abortion.

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Title: Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion
Authors: Talieh Kazerooni
Nasrin Asadi
Leila Jadid
Marjaneh Kazerooni
Alireza Ghanadi
Fariborz Ghaffarpasand
Yasaman Kazerooni
Jaleh Zolghadr
Publication date: 01-12-2009
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 11-12/2009
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-009-9361-3

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Evaluation of sperm’s chromatin quality with acridine orange test, chromomycin A3 and aniline blue staining in couples with unexplained recurrent abortion

Abstract

Objective

Methods

Result

Conclusion

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Objective

Methods

Result

Conclusion

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