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Journal of Assisted Reproduction and Genetics

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01-03-2009 | ANDROLOGY

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Authors: Roberta Maria Fariello, Paula Toni Del Giudice, Deborah Montagnini Spaine, Renato Fraietta, Ricardo Pimenta Bertolla, Agnaldo Pereira Cedenho

Published in: Journal of Assisted Reproduction and Genetics | Issue 2-3/2009

Abstract

Purpose

To assess the effect of leukocytospermia and semen processing on sperm DNA and mitochondria.

Methods

Twenty-two patients with and 41 without leukocytospermia were included. Sperm DNA fragmentation was assessed by the Comet assay, and mitochondrial activity by a colorimetric method for active mitochondria. Semen was processed using Percoll, and motility, DNA fragmentation, and mitochondrial activity were analyzed pre- and post-processing.

Results

No differences were observed in age, abstinence, volume, sperm morphology, progressive motility, concentration, and vitality (p > 0.10). Variables were grouped according to time (pre- vs post-processing) and group (leukocytospermia vs non-leukocytospermia) because no interactions could be observed. Leukocytospermia was associated to increased DNA fragmentation, while semen processing led to a decrease in DNA fragmentation and to increased mitochondrial activity.

Conclusion

While semen processing selects sperm with higher rates of DNA integrity independent of the presence or absence of leukocytes in semen, samples without leukocytospermia present more sperm without DNA fragmentation. Semen processing also selects sperm with higher mitochondrial activity.

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Title: Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity
Authors: Roberta Maria Fariello
Paula Toni Del Giudice
Deborah Montagnini Spaine
Renato Fraietta
Ricardo Pimenta Bertolla
Agnaldo Pereira Cedenho
Publication date: 01-03-2009
Publisher: Springer US
Published in: Journal of Assisted Reproduction and Genetics / Issue 2-3/2009
Print ISSN: 1058-0468
Electronic ISSN: 1573-7330
DOI: https://doi.org/10.1007/s10815-008-9288-0

At a glance: The STEP trials

Springer Medicine

Effect of leukocytospermia and processing by discontinuous density gradient on sperm nuclear DNA fragmentation and mitochondrial activity

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The STEP trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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