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International Ophthalmology
Published in: International Ophthalmology 9/2020

Open Access 01-09-2020 | Edema | Original Paper

Relationships between the clinical characteristics and copy numbers of DNA of cytomegalovirus determined by real-time PCR

Authors: Ayano Yoshimura, Kaoru Araki-Sasaki, Noriko Toyokawa, Rho Fujiwara, Nobuo Jho, Fumi Gomi

Published in: International Ophthalmology | Issue 9/2020

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Abstract

Purpose

To determine whether there is a correlation between the clinicals characteristics including various types of keratic precipitates and the copy numbers of the DNA of cytomegalovirus (CMV) in eyes with CMV corneal endotheliitis.

Methods

We reviewed the medical charts of four cases of corneal endotheliitis that were CMV-positive. We have classified types of clinical phenomenon into four types: coin-shaped KPs, sectoral corneal edema with or without Khodadoust line-like KPs, mutton-fat KPs, and fine KPs and have graded their severity. We also determined the copy numbers of the DNA of CMV in the aqueous humor by real-time polymerase chain reaction before and during the treatment. We evaluated the correlation between the patterns of clinical characteristics and copy number of the DNA of CMV.

Results

There were clinical improvements in all eyes following topical ganciclovir in conjunction with low dose of topical steroid treatment, with or without oral valganciclovir. The clinical characteristics and the copy numbers of the DNA of CMV varied during the treatment period. The presence of coin-shaped KPs was correlated with high copy numbers (105–103 copies/ml) of the DNA of CMV. The copy numbers of the DNA of CMV with sectoral corneal edema with or without Khodadoust line-like KPs ranged from 104 to 102 copies/ml, and it was occasionally accompanied by high intraocular pressure. Mutton-fat KPs were observed inferiorly, sometimes together with coin-shaped KPs and sectoral corneal edema, or solely. The copy numbers in eyes with mutton-fat KPs varied and occasionally less than the cutoff level. Fine-pigmented KPs were observed after the resolution of the endotheliitis, and no DNA of CMV was detected in the aqueous humor.

Conclusions

Careful observations of the clinical characteristics such as the KPs and corneal edema might be helpful in estimating the amount of the DNA of CMV in eyes with corneal endotheliitis.
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Metadata
Title
Relationships between the clinical characteristics and copy numbers of DNA of cytomegalovirus determined by real-time PCR
Authors
Ayano Yoshimura
Kaoru Araki-Sasaki
Noriko Toyokawa
Rho Fujiwara
Nobuo Jho
Fumi Gomi
Publication date
01-09-2020
Publisher
Springer Netherlands
Keyword
Edema
Published in
International Ophthalmology / Issue 9/2020
Print ISSN: 0165-5701
Electronic ISSN: 1573-2630
DOI
https://doi.org/10.1007/s10792-020-01412-6

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