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01-02-2020 | Original Paper

CDKN2A, CDK1, and CCNE1 overexpression in sebaceous gland carcinoma of eyelid

Authors: Tatsuya Yunoki, Tetsushi Hirano, Yoshiaki Tabuchi, Yukihiro Furusawa, Misako Torigoe, Takahiko Nakajima, Johji Imura, Atsushi Hayashi

Published in: International Ophthalmology | Issue 2/2020

Abstract

Purpose

To investigate the overexpression of genes in sebaceous gland carcinoma (SGC) of the eyelid compared to sebaceous adenoma of the eyelid in order to elucidate the molecular mechanism underlying pathogenesis.

Methods

We performed histopathological examination of eyelid tissues surgically removed from four patients diagnosed with SGC (cases 1–3) and sebaceous adenoma (case 4) of the eyelid. Next, we performed global gene expression analysis of surgical tissue samples using a GeneChip^® system and the Ingenuity Pathways Knowledge Base. The results of the GeneChip^® analysis were explored with quantitative real-time polymerase chain reaction (qRT-PCR) analysis.

Results

In the SGC samples, we found that 211, 199, and 199 genes, respectively, showed ≥ 2.0-fold higher expression than those in the sebaceous adenoma sample (case 4); 194 genes were common to all three SGC samples. For the 194 genes with upregulated expression, functional category analysis showed that SGC of the eyelid employed a unique gene network, including cyclin-dependent kinase inhibitor 2A (CDKN2A), cyclin-dependent kinase 1 (CDK1), and cyclin E1 (CCNE1), which are related to cell cycle progression, incidence of tumor, and cell viability. Furthermore, qRT-PCR analysis showed that the expression levels of CDKN2A, CDK1, and CCNE1 were significantly upregulated in all SGC cases compared to those in the sebaceous adenoma case. These data were similar to the results of microarray analysis.

Conclusion

Overexpression of cell cycle-related genes CDKN2A, CDK1, CCNE1, and their gene network may help elucidate the pathogenic pathway of SGC of the eyelid at the molecular level.

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Title: CDKN2A, CDK1, and CCNE1 overexpression in sebaceous gland carcinoma of eyelid
Authors: Tatsuya Yunoki
Tetsushi Hirano
Yoshiaki Tabuchi
Yukihiro Furusawa
Misako Torigoe
Takahiko Nakajima
Johji Imura
Atsushi Hayashi
Publication date: 01-02-2020
Publisher: Springer Netherlands
Published in: International Ophthalmology / Issue 2/2020
Print ISSN: 0165-5701
Electronic ISSN: 1573-2630
DOI: https://doi.org/10.1007/s10792-019-01185-7

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

CDKN2A, CDK1, and CCNE1 overexpression in sebaceous gland carcinoma of eyelid

Abstract

Purpose

Methods

Results

Conclusion

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusion

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