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International Ophthalmology
Published in: International Ophthalmology 5/2016

01-10-2016 | Original Paper

Outcomes of different concentrations of human amniotic fluid in a keratoconjunctivitis sicca-induced mouse model

Authors: Guilherme G. Quinto, Juan Castro-Combs, Li Li, Navin Gupta, Mauro Campos, Ashley Behrens

Published in: International Ophthalmology | Issue 5/2016

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Abstract

To compare the effects of different concentrations of topical human amniotic fluid (HAF) in a mouse model of dry eye, forty C57BL/6 mice were divided into 4 treatment groups: 20 % HAF, 50 % HAF, 100 % HAF, and isotonic salt solution (control). Dry eye was induced by an injection of botulinum toxin B into the lacrimal gland. Tear production, ocular surface fluorescein staining, and blink rate were evaluated in each mouse at 5 time points during a 4-week period. Goblet cell density was assessed in stained histological sections. Regarding tear production, 20, 50, and 100 % HAF groups were all different from the control group (P < 0.001) at week 1. However, there were no statistically significant differences between the 20, 50, and 100 % HAF groups. At week 2, 20, 50, and 100 % HAF groups had significant improvement in staining score and were significantly different from the control group (P = 0.047, P = 0.005, and P = 0.001, respectively). No difference in spontaneous blink rate was observed between groups, at any time point. Goblet cell density was significantly decreased in the control group compared to the HAF treatment groups. All tested concentrations of topical HAF were effective and superior than the control in this keratoconjunctivitis sicca-induced mouse model. Further studies are needed to evaluate the effects of HAF on the human ocular surface.
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Metadata
Title
Outcomes of different concentrations of human amniotic fluid in a keratoconjunctivitis sicca-induced mouse model
Authors
Guilherme G. Quinto
Juan Castro-Combs
Li Li
Navin Gupta
Mauro Campos
Ashley Behrens
Publication date
01-10-2016
Publisher
Springer Netherlands
Published in
International Ophthalmology / Issue 5/2016
Print ISSN: 0165-5701
Electronic ISSN: 1573-2630
DOI
https://doi.org/10.1007/s10792-016-0180-0

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