Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
International Ophthalmology
Published in: International Ophthalmology 3/2016

01-06-2016 | Case Report

Detection of Mycobacterium tuberculosis with nested polymerase chain reaction analysis in enucleated eye ball in Eales’ disease

Authors: Aditya Verma, Jyotirmay Biswas, L. Dhanurekha, R. Gayathri, K. Lily Therese

Published in: International Ophthalmology | Issue 3/2016

Login to get access

Abstract

Nested polymerase chain reaction (nPCR) was performed on enucleated eyeball for detection of Mycobacterium tuberculosis (M. tb) genome in a patient with Eales’ disease. PCR analysis in all previous studies has been done mainly using aqueous, vitreous and epiretinal membranes from these patients. Paraffin wax embedded tissue section of the enucleated eyeball was analyzed by histopathology and nPCR targeting MPB64 gene and IS6110 region of M. tb genome. Lymphocytic infiltration was seen in the vitreous, iris and the retinal tissue. Ziehl Neelsen stain was negative for acid fast bacilli. Caseation necrosis was not seen in any section. Agarose gel electrophoretogram showed positive results with 200 bp specific amplified product targeting MPB64 gene, whereas nPCR targeting IS6110 region was negative. Since biopsy proven M. tb is extremely difficult in ocular tissues due to extensive necrosis, the nPCR technique aided in the diagnosis.
Literature
1.
Singh R, Toor P, Parchand S et al (2012) Quantitative polymerase chain reaction for Mycobacterium tuberculosis in so-called Eales’ disease. Ocul Immunol Inflamm. 20(3):153–157CrossRefPubMed
2.
Biswas J, Ravi RK, Naryanasamy A et al (2013) Eales’ disease—current concepts in diagnosis and management. J Ophthalmic Inflamm Infect. 13(1):11CrossRef
3.
Therese KL, Deepa P, Therese J et al (2007) Association of mycobacteria with Eales’ disease. Indian J Med Res 126(1):56–62PubMed
4.
Biswas J, Kumar SK, Rupauliha P et al (2002) Detection of Mycobacterium tuberculosis by nested polymerase chain reaction in a case of subconjunctival tuberculosis. Cornea 21(1):123–125CrossRefPubMed
5.
Wroblewski KJ, Hidayat AA, Neafie RC, Rao NA, Zapor M (2011) Ocular tuberculosis: a clinicopathologic and molecular study. Ophthalmology 118(4):772–777CrossRefPubMed
6.
Therese KL, Gayathri R, Dhanurekha L et al (2013) Diagnostic appraisal of simultaneous application of two nested PCRs targeting MPB64 gene and IS6110 region for rapid detection of M. tuberculosis genome in culture proven clinical specimens. Indian J Med Microbiol. 31(4):366–369CrossRefPubMed
7.
Kotake S, Kimura K, Yoshikawa K et al (1994) Polymerase chain reaction for detection of Mycobacterium tuberculosis in ocular tuberculosis. Am J Ophthalmol 117(6):805–806CrossRefPubMed
8.
Gupta A, Gupta V, Arora S et al (2001) PCR-positive tubercular retinal vasculitis. Retina 21(5):435–444CrossRefPubMed
9.
Madhavan HN, Therese KL, Gunisha P (2000) Polymerase chain reaction for detection of Mycobacterium tuberculosis in epiretinal membrane in Eales’ disease. Invest Ophthalmol Vis Sci 41(3):822–825PubMed
10.
Biswas J, Therese L, Madhavan HN (1999) Use of polymerase chain reaction in detection of Mycobacterium tuberculosis complex DNA from vitreous sample of Eales’ disease. Br J Ophthalmol 83(8):994CrossRefPubMedPubMedCentral
Metadata
Title
Detection of Mycobacterium tuberculosis with nested polymerase chain reaction analysis in enucleated eye ball in Eales’ disease
Authors
Aditya Verma
Jyotirmay Biswas
L. Dhanurekha
R. Gayathri
K. Lily Therese
Publication date
01-06-2016
Publisher
Springer Netherlands
Published in
International Ophthalmology / Issue 3/2016
Print ISSN: 0165-5701
Electronic ISSN: 1573-2630
DOI
https://doi.org/10.1007/s10792-015-0144-9

Other articles of this Issue 3/2016

International Ophthalmology 3/2016 Go to the issue

Original Paper

Intraocular pressure control after the implantation of a second Ahmed glaucoma valve

Review

A review of unilateral acute idiopatic maculopathy related to hand–foot–mouth disease with a representative case

Review

Primary orbital yolk sac tumor: report of a case and review of literature

Original Paper

Conjunctival and corneal sensitivity in patients under topical antiglaucoma treatment

Original Paper

Effect on contrast sensitivity after clear, yellow and orange intraocular lens implantation

Original Paper

Risk factors for intra-operative complications during phacoemulsification performed by residents