Top

Published in:

01-10-2018 | Original Article

Curcumin ameliorates palmitate-induced inflammation in skeletal muscle cells by regulating JNK/NF-kB pathway and ROS production

Authors: Asie Sadeghi, Atefeh Rostamirad, Shadisadat Seyyedebrahimi, Reza Meshkani

Published in: Inflammopharmacology | Issue 5/2018

Abstract

Curcumin, a natural polyphenol compound, has the beneficial effects on several diseases such as metabolic syndrome, cancer, and diabetes. The anti-inflammatory property of curcumin has been demonstrated in different cells; however, its role in prevention of palmitate-induced inflammation in skeletal muscle C2C12 cells is not known. In this study, we examined the effect of curcumin on the inflammatory responses stimulated by palmitate in C2C2 cells. The results showed that palmitate upregulated the mRNA expression and protein release of IL-6 and TNF-α cytokines in C2C12 cells, while pretreatment with curcumin was able to attenuate the effect of palmitate on inflammatory cytokines. The anti-inflammatory effect of curcumin was associated with the repression of phosphorylation of IKKα-IKKβ, and JNK. Palmitate also caused an increase in reactive oxygen species (ROS) level that curcumin abrogated it. Collectively, these findings suggest that curcumin may represent a promising therapy for prevention of inflammation in skeletal muscle cells.

Barma P et al (2009) Lipid induced overexpression of NF-κB in skeletal muscle cells is linked to insulin resistance. Biochim Biophys Acta 1792:190–200CrossRef

Boden G, Shulman G (2002) Free fatty acids in obesity and type 2 diabetes: defining their role in the development of insulin resistance and β-cell dysfunction. Eur J Clin Invest 32:14–23CrossRef

Coll T et al (2008) Oleate reverses palmitate-induced insulin resistance and inflammation in skeletal muscle cells. J Biol Chem 283:11107–11116CrossRef

Deng Y-T, Chang T-W, Lee M-S, Lin J-K (2012) Suppression of free fatty acid-induced insulin resistance by phytopolyphenols in C2C12 mouse skeletal muscle cells. J Agric Food Chem 60:1059–1066CrossRef

Devi YS, DeVine M, DeKuiper J, Ferguson S, Fazleabas AT (2015) Inhibition of IL-6 signaling pathway by curcumin in uterine decidual cells. PLoS ONE 10:e0125627CrossRef

Frost RA, Nystrom GJ, Lang CH (2002) Lipopolysaccharide regulates proinflammatory cytokine expression in mouse myoblasts and skeletal muscle. Am J Physiol-Regul Integr Comp Physiol 283:R698–R709CrossRef

Fu Y, Zheng S, Lin J, Ryerse J, Chen A (2008) Curcumin protects the rat liver from CCl4-caused injury and fibrogenesis by attenuating oxidative stress and suppressing inflammation. Mol Pharmacol 73:399–409CrossRef

Geng S et al (2017) Curcumin attenuates BPA-induced insulin resistance in HepG2 cells through suppression of JNK/p38 pathways. Toxicol Lett 272:75–83CrossRef

Gupta SC, Patchva S, Aggarwal BB (2013) Therapeutic roles of curcumin: lessons learned from clinical trials. AAPS J 15:195–218CrossRef

Inoguchi T et al (2000) High glucose level and free fatty acid stimulate reactive oxygen species production through protein kinase C—dependent activation of NAD (P) H oxidase in cultured vascular cells. Diabetes 49:1939–1945CrossRef

Khan IM et al (2015) Intermuscular and perimuscular fat expansion in obesity correlates with skeletal muscle T cell and macrophage infiltration and insulin resistance. Int J Obes 39:1607CrossRef

Khodabandehloo H, Gorgani-Firuzjaee S, Panahi G, Meshkani R (2016) Molecular and cellular mechanisms linking inflammation to insulin resistance and beta-cell dysfunction. Transl Res 167:228–256. https://doi.org/10.1016/j.trsl.2015.08.011 CrossRefPubMed

Klotz LO, Pellieux C, Briviba K, Pierlot C, Aubry JM, Sies H (1999) Mitogen-activated protein kinase (p38-, JNK-, ERK-) activation pattern induced by extracellular and intracellular singlet oxygen and UVA. FEBS J 260:917–922

Kowluru RA, Kanwar M (2007) Effects of curcumin on retinal oxidative stress and inflammation in diabetes. Nutr Metab 4:8CrossRef

Koyama T et al (2011) SIRT3 attenuates palmitate-induced ROS production and inflammation in proximal tubular cells. Free Radical Biol Med 51:1258–1267CrossRef

Kuhad A, Pilkhwal S, Sharma S, Tirkey N, Chopra K (2007) Effect of curcumin on inflammation and oxidative stress in cisplatin-induced experimental nephrotoxicity. J Agric Food Chem 55:10150–10155CrossRef

Lang CH, Silvis C, Deshpande N, Nystrom G, Frost RA (2003) Endotoxin stimulates in vivo expression of inflammatory cytokines tumor necrosis factor alpha, interleukin-1β,-6, and high-mobility-group protein-1 in skeletal muscle. Shock 19:538–546CrossRef

Lawrence T (2009) The nuclear factor NF-κB pathway in inflammation. Cold Spring Harbor Perspect Biol 1:a001651CrossRef

Ma F, Liu F, Ding L, You M, Yue H, Zhou Y, Hou Y (2017) Anti-inflammatory effects of curcumin are associated with down regulating microRNA-155 in LPS-treated macrophages and mice. Pharm Biol 55:1263–1273CrossRef

Maithilikarpagaselvi N, Sridhar MG, Swaminathan RP, Zachariah B (2016) Curcumin prevents inflammatory response, oxidative stress and insulin resistance in high fructose fed male Wistar rats: potential role of serine kinases. Chem Biol Interact 244:187–194CrossRef

Maloney E et al (2009) Activation of NF-κB by palmitate in endothelial cells. Arterioscler Thromb Vasc Biol 29:1370–1375CrossRef

Martins AR et al (2012) Mechanisms underlying skeletal muscle insulin resistance induced by fatty acids: importance of the mitochondrial function. Lipids Health Dis 11:30CrossRef

Meshkani R, Vakili S (2016) Tissue resident macrophages: key players in the pathogenesis of type 2 diabetes and its complications. Clinica Chimica Acta 462:77–89. https://doi.org/10.1016/j.cca.2016.08.015 CrossRef

Na L-X, Zhang Y-L, Li Y, Liu L-Y, Li R, Kong T, Sun C-H (2011) Curcumin improves insulin resistance in skeletal muscle of rats. Nutr Metab Cardiovasc Dis 21:526–533CrossRef

Nakamura S et al (2009) Palmitate induces insulin resistance in H4IIEC3 hepatocytes through reactive oxygen species produced by mitochondria. J Biol Chem 284:14809–14818CrossRef

Panahi Y, Sahebkar A, Parvin S, Saadat A (2012) A randomized controlled trial on the anti-inflammatory effects of curcumin in patients with chronic sulphur mustard-induced cutaneous complications. Ann Clin Biochem 49:580–588CrossRef

Panahi Y, Hosseini MS, Khalili N, Naimi E, Majeed M, Sahebkar A (2015) Antioxidant and anti-inflammatory effects of curcuminoid-piperine combination in subjects with metabolic syndrome: a randomized controlled trial and an updated meta-analysis. Clin Nutr 34:1101–1108CrossRef

Park JM, Lee JS, Song JE, Sim YC, Ha S-J, Hong EK (2015) Cytoprotective effect of hispidin against palmitate-induced lipotoxicity in C2C12 myotubes. Molecules 20:5456–5467CrossRef

Pillon NJ, Arane K, Bilan PJ, Chiu TT, Klip A (2012) Muscle cells challenged with saturated fatty acids mount an autonomous inflammatory response that activates macrophages. Cell Commun Signal 10:30CrossRef

Reaven GM (1991) Insulin resistance and compensatory hyperinsulinemia: role in hypertension, dyslipidemia, and coronary heart disease. Am Heart J 121:1283–1288CrossRef

Sadeghi A, Ebrahimi S, Sadat S, Golestani A, Meshkani R (2017) Resveratrol ameliorates palmitate-induced inflammation in skeletal muscle cells by attenuating oxidative stress and JNK/NF-κB pathway in a SIRT1-independent mechanism. J Cell Biochem 118(9):2654–2663CrossRef

Shao-Ling W, Ying L, Ying W, Yan-Feng C, Li-Xin N, Song-Tao L, Chang-Hao S (2009) Curcumin, a potential inhibitor of up-regulation of TNF-alpha and IL-6 induced by palmitate in 3T3-L1 adipocytes through NF-kappaB and JNK pathway. Biomed Environ Sci 22:32–39CrossRef

Sharma R, Gescher A, Steward W (2005) Curcumin: the story so far. Eur J Cancer 41:1955–1968CrossRef

Tak PP, Firestein GS (2001) NF-κB: a key role in inflammatory diseases. J Clin Investig 107:7–11CrossRef

Varma V et al (2009) Muscle inflammatory response and insulin resistance: synergistic interaction between macrophages and fatty acids leads to impaired insulin action. Am J Physiol-Endocrinol Metab 296:E1300–E1310CrossRef

Varma SR, Sivaprakasam TO, Mishra A, Prabhu S, Rafiq M, Rangesh P (2017) Imiquimod-induced Psoriasis-like inflammation in differentiated Human keratinocytes: its evaluation using curcumin. Eur J Pharmacol 813:33–41CrossRef

Xu Y, Liu L (2017) Curcumin alleviates macrophage activation and lung inflammation induced by influenza virus infection through inhibiting the NF-κB signaling pathway. Influenza Respir Viruses 11(5):457–463CrossRef

Yang M et al (2013) Saturated fatty acid palmitate-induced insulin resistance is accompanied with myotube loss and the impaired expression of health benefit myokine genes in C2C12 myotubes. Lipids Health Dis 12:104CrossRef

Zhang J, Wang X, Vikash V, Ye Q, Wu D, Liu Y, Dong W (2016) ROS and ROS-mediated cellular signaling. Oxid Med Cell Longev. https://doi.org/10.1155/2016/4350965 CrossRefPubMedPubMedCentral

Title: Curcumin ameliorates palmitate-induced inflammation in skeletal muscle cells by regulating JNK/NF-kB pathway and ROS production
Authors: Asie Sadeghi
Atefeh Rostamirad
Shadisadat Seyyedebrahimi
Reza Meshkani
Publication date: 01-10-2018
Publisher: Springer International Publishing
Published in: Inflammopharmacology / Issue 5/2018
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-018-0466-0

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Curcumin ameliorates palmitate-induced inflammation in skeletal muscle cells by regulating JNK/NF-kB pathway and ROS production

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 5/2018

Grape polyphenols corrects ageing-related detriments in neutrophil functionality via modulation of specific molecular targets

Labisia pumila prevented osteoarthritis cartilage degeneration by attenuating joint inflammation and collagen breakdown in postmenopausal rat model

Influence of disease-modifying antirheumatic drugs on oxidative and nitrosative stress in patients with rheumatoid arthritis

Brazilian green propolis hydroalcoholic extract reduces colon damages caused by dextran sulfate sodium-induced colitis in mice

Curcumin–galactomannoside complex inhibits pathogenesis in Ox-LDL-challenged human peripheral blood mononuclear cells

Efficacy analysis of hydroxychloroquine therapy in systemic lupus erythematosus: a study on disease activity and immunological biomarkers