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01-04-2019 | Original Article

Anti-inflammatory and antinociceptive properties of the hydroalcoholic fractions from the leaves of Annona crassiflora Mart. in mice

Authors: Cristina da Costa Oliveira, Natália Alves de Matos, Clarice de Carvalho Veloso, Gisele Avelar Lage, Lúcia Pinheiro Santos Pimenta, Igor Dimitri Gama Duarte, Thiago Roberto Lima Romero, André Klein, Andrea de Castro Perez

Published in: Inflammopharmacology | Issue 2/2019

Abstract

Background

Annona crassiflora Mart., popularly known as “Araticum”, is a native tree of the Brazilian Cerrado used in folk medicine for treatment of pain and inflammatory diseases. We proposed to analyze analgesic and anti-inflammatory properties of the filtrate (F1) and the precipitate (F2) of the hydroalcoholic fraction from the leaves of Annona crassiflora Mart. in mice.

Materials and methods

Swiss mice were submitted to formalin-induced nociception test and tail-flick reflex test, to assess antinociceptive properties, and to the rota-rod test, for motor performance analyses. To evaluate anti-inflammatory properties, F1 and F2 were orally administered 1 h prior to the intrathoracic injection of carrageenan, zymosan, LPS, CXCL8, or vehicle in Balb/c mice and neutrophil infiltration was evaluated 4 h after injection.

Results

F1 and F2 reduced the licking time in the second phase of formalin-induced nociception test, but only F2 showed a dose-dependent response. Neither F1 nor F2 reduced the latency time in the tail-flick reflex test. In addition, motor performance alteration was not observed in F1- or F2-treated mice. F2 treatment significantly inhibited the neutrophilia induced by carrageenan, LPS, or CXCL8, but not zymosan.

Conclusions

The experimental data demonstrated that hydroalcoholic fractions of Annona crassiflora Mart. leaves have remarkable anti-inflammatory and antinociceptive activities.

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Title: Anti-inflammatory and antinociceptive properties of the hydroalcoholic fractions from the leaves of Annona crassiflora Mart. in mice
Authors: Cristina da Costa Oliveira
Natália Alves de Matos
Clarice de Carvalho Veloso
Gisele Avelar Lage
Lúcia Pinheiro Santos Pimenta
Igor Dimitri Gama Duarte
Thiago Roberto Lima Romero
André Klein
Andrea de Castro Perez
Publication date: 01-04-2019
Publisher: Springer International Publishing
Published in: Inflammopharmacology / Issue 2/2019
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-017-0426-0

At a glance: The ONWARDS insulin icodec trials

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Anti-inflammatory and antinociceptive properties of the hydroalcoholic fractions from the leaves of Annona crassiflora Mart. in mice

Abstract

Background

Materials and methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

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Abstract

Background

Materials and methods

Results

Conclusions

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