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01-04-2011 | Research Article

Anti-inflammatory properties of Doxycycline and Minocycline in experimental models: an in vivo and in vitro comparative study

Authors: Liz M. Leite, Antônio Germano G. Carvalho, Pollyanna L. F. Tavares Ferreira, Igor Xavier Pessoa, Danilo O. Gonçalves, Amanda de Araújo Lopes, Jean Guilherme dos Santos Góes, Victor Costa de Castro Alves, Luzia Kalyne A. M. Leal, Gerly Anne Brito, Glauce S. B. Viana

Published in: Inflammopharmacology | Issue 2/2011

Abstract

Aims and methods

Minocycline (Mino) and doxycycline (Dox) are second generation tetracyclines known to present several other effects, which are independent from their antimicrobial activities. We studied in a comparative way the anti-inflammatory effects of Mino and Dox, on acute models of peripheral inflammation in rodents (formalin test and peritonitis in mice, and carrageenan-induced paw oedema in rats). Immunohistochemical assays for TNF-alpha and iNOS in rat paws of carrageenan-induced oedema were also carried out as well as in vitro assays for myeloperoxidase (MPO) and lactate dehydrogenase (LDH). Furthermore, antioxidant activities were evaluated by the DPPH assay.

Results

In the formalin test although Mino and Dox (1, 5, 10 and 25 mg/kg, i.p.) inhibited the first phase, they acted predominantly on the second phase of the test, where inhibition of the licking time close to 80% were observed. Mino and Dox were very efficacious in reducing the carrageenan-induced paw oedema in rats (10, 25 and 50 mg/kg, i.p.) and carrageenan-induced leucocyte migration (1 and 5 mg/kg, i.p.) to mice peritoneal cavities. Besides, they also significantly inhibited MPO and LDH releases at doses ranging from 0.001 to 1 μg/ml. Thus, in general, the anti-inflammatory activity of Dox was higher as compared to that of Mino, although the radical scavenging activity of Mino was of a magnitude 10 times higher.

Conclusions

Our data indicate that anti-inflammatory and antioxidant effects, involve the inhibition of iNOS and TNF-alpha, among other properties, and these encourage clinical studies of these compounds for new therapeutic applications, especially those were inflammation plays a role.

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Title: Anti-inflammatory properties of Doxycycline and Minocycline in experimental models: an in vivo and in vitro comparative study
Authors: Liz M. Leite
Antônio Germano G. Carvalho
Pollyanna L. F. Tavares Ferreira
Igor Xavier Pessoa
Danilo O. Gonçalves
Amanda de Araújo Lopes
Jean Guilherme dos Santos Góes
Victor Costa de Castro Alves
Luzia Kalyne A. M. Leal
Gerly Anne Brito
Glauce S. B. Viana
Publication date: 01-04-2011
Publisher: SP Birkhäuser Verlag Basel
Published in: Inflammopharmacology / Issue 2/2011
Print ISSN: 0925-4692
Electronic ISSN: 1568-5608
DOI: https://doi.org/10.1007/s10787-011-0077-5

At a glance: The ONWARDS insulin icodec trials

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Anti-inflammatory properties of Doxycycline and Minocycline in experimental models: an in vivo and in vitro comparative study

Abstract

Aims and methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Aims and methods

Results

Conclusions

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