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01-02-2019 | Original Research Article

Toxicity and in vivo release profile of sirolimus from implants into the vitreous of rabbits’ eyes

Authors: Mayara Rodrigues Brandão De Paiva, Nayara Almeida Lage, Maria Carolina Andrade Guerra, Marcos Paulo Gomes Mol, Marcela Coelho Silva Ribeiro, Gustavo De Oliveira Fulgêncio, Dawidson A. Gomes, Isabela Da Costa César, Sílvia Ligório Fialho, Armando Silva-Cunha

Published in: Documenta Ophthalmologica | Issue 1/2019

Abstract

Purpose

To assess the in vivo release profile and the retinal toxicity of a poly (lactic-co-glycolic acid) (PLGA) sustained-release sirolimus (SRL) intravitreal implant in normal rabbit eyes.

Methods

PLGA intravitreal implants containing or not SRL were prepared, and the viability of ARPE-19 and hES-RPE human retinal cell lines was examined after 24 and 72 h of exposure to implants. New Zealand rabbits were randomly divided into two groups that received intravitreal implants containing or not SRL. At each time point (1–8 weeks), four animals from the SRL group were euthanized, the vitreous was collected, and drug concentration was calculated. Clinical evaluation of the eyes was performed weekly for 8 weeks after administration. Electroretinography (ERG) was recorded in other eight animals, four for each group, at baseline and at 24 h, 1, 4, 6, and 8 weeks after the injection. ERG was carried out using scotopic and photopic protocols. The safety of the implants was assessed using statistical analysis of the ERG parameters (a and b waves, a and b implicit time, B/A ratio, oscillatory potential, and Naka–Rushton analysis) comparing the functional integrity of the retina between the PLGA and SRL-PLGA groups. After the last electrophysiological assessment, the rabbits were euthanized and retinal histopathology was realized.

Results

After 24 and 72 h of incubation with PLGA or SRL-PLGA implants, ARPE-19 and hES-RPE cells showed viability over 70%. The maximum concentration of SRL (199.8 ng/mL) released from the device occurred within 4 weeks. No toxic effects of the implants or increase in the intraocular pressure was observed through clinical evaluation of the eye. ERG responses showed no significant difference between the eyes that received PLGA or SRL-PLGA implants at baseline and throughout the 8 weeks of follow-up. No remarkable difference in retinal histopathology was detected in rabbit eyes treated with PLGA or SRL-PLGA implants.

Conclusions

Intravitreal PLGA or SRL-PLGA implants caused no significant reduction in cell viability and showed no evident toxic effect on the function or structure of the retina of the animals. SRL was released from PLGA implant after application in the vitreous of rabbits during 8 weeks.

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Title: Toxicity and in vivo release profile of sirolimus from implants into the vitreous of rabbits’ eyes
Authors: Mayara Rodrigues Brandão De Paiva
Nayara Almeida Lage
Maria Carolina Andrade Guerra
Marcos Paulo Gomes Mol
Marcela Coelho Silva Ribeiro
Gustavo De Oliveira Fulgêncio
Dawidson A. Gomes
Isabela Da Costa César
Sílvia Ligório Fialho
Armando Silva-Cunha
Publication date: 01-02-2019
Publisher: Springer Berlin Heidelberg
Published in: Documenta Ophthalmologica / Issue 1/2019
Print ISSN: 0012-4486
Electronic ISSN: 1573-2622
DOI: https://doi.org/10.1007/s10633-018-9664-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Toxicity and in vivo release profile of sirolimus from implants into the vitreous of rabbits’ eyes

Abstract

Purpose

Methods

Results

Conclusions

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Purpose

Methods

Results

Conclusions

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