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Documenta Ophthalmologica
Published in: Documenta Ophthalmologica 1/2013

01-02-2013 | Original Research Article

Characteristics of late negative ERG responses elicited by sawtooth flicker

Authors: Sowjanya Gowrisankaran, Mohamed A. Genead, Anastasios Anastasakis, Kenneth R. Alexander

Published in: Documenta Ophthalmologica | Issue 1/2013

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Abstract

Background

This study aimed to determine whether the properties of the late negative responses (LNRs) of the electroretinogram (ERG) elicited by sawtooth flicker are consistent with the characteristics of the photopic negative response generated by a light pulse (PhNRpulse).

Methods

ERG recordings were obtained from 10 visually normal individuals and from 6 patients with optic atrophy (OA) in response to 8-Hz rapid-on and rapid-off sawtooth flicker and to brief (4 ms) light pulses. All stimuli were either long wavelength (R), middle wavelength (G), or a combination of equal luminances of long and middle wavelengths (Y) presented on a short-wavelength, rod-saturating adapting field. Amplitudes of LNRs were obtained in response to rapid-on (LNRon) and rapid-off (LNRoff) sawtooth flicker and were also derived from the sum of the ERG waveforms to the two sawtooth phases (LNRadd).

Results

For the control subjects, PhNRpulse amplitude varied with stimulus wavelength, being largest in response to a long-wavelength pulse, as expected. However, the amplitudes of LNRon, LNRoff, and LNRadd were not significantly different for R, Y, and G sawtooth flicker. Despite the absence of a chromatic effect, LNRoff and LNRadd amplitudes were significantly smaller in the OA patients than in the controls, similar to the results for the PhNRpulse, implying an inner retinal origin for the LNRoff and LNRadd. However, LNRon amplitudes did not differ significantly between the OA patients and controls, although there was a significant correlation between the LNRon and PhNRpulse for R stimuli.

Conclusion

We conclude that LNRoff and LNRadd but not LNRon can be useful measures to assess the integrity of the inner retina that can complement the PhNRpulse.
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Metadata
Title
Characteristics of late negative ERG responses elicited by sawtooth flicker
Authors
Sowjanya Gowrisankaran
Mohamed A. Genead
Anastasios Anastasakis
Kenneth R. Alexander
Publication date
01-02-2013
Publisher
Springer-Verlag
Published in
Documenta Ophthalmologica / Issue 1/2013
Print ISSN: 0012-4486
Electronic ISSN: 1573-2622
DOI
https://doi.org/10.1007/s10633-012-9352-z

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