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Japanese Journal of Ophthalmology
Published in: Japanese Journal of Ophthalmology 1/2021

01-01-2021 | Laboratory Investigation

Role of oncostatin M in the pathogenesis of vernal keratoconjunctivitis: focus on tissue remodeling

Authors: Keitaro Mashimo, Ayumi Usui-Ouchi, Yousuke Ito, Rei Wakasa-Arai, Norihiko Yokoi, Satoshi Kawasaki, Akira Murakami, Akira Matsuda, Nobuyuki Ebihara

Published in: Japanese Journal of Ophthalmology | Issue 1/2021

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Abstract

Purpose

Vernal keratoconjunctivitis (VKC) is a severe and recurrent allergic conjunctivitis, the mechanism of which is not well understood. In this study, we investigated the role of oncostatin M (OSM) in the pathogenesis of VKC, with a focus on tissue remodeling.

Study design

Clinical and experimental.

Patients and methods

The OSM concentrations in tear fluid samples obtained from VKC patients and healthy controls were measured using ELISA, and the expression of OSM mRNA and protein in giant papillae resected from VKC patients was investigated using RT-PCR and immunohistochemistry, respectively. In cultured human conjunctival epithelial cells (HconEpiCs), expression of OSM receptor β (OSMRβ) was detected using immunocytochemical and FACS analyses. Finally, we investigated whether recombinant OSM activated STAT1 and STAT3 to induce the expression of various genes related to tissue remodeling in HconEpiCs, by using Western blot analysis, microarray analysis, and RT-PCR.

Results

The OSM concentration was higher in the tear fluid of VKC patients than in that of the healthy controls, and strong expression of OSM mRNA was found in the giant papillae. We also detected T cells expressing OSM in the giant papillae. In addition, HconEpiCs showed surface expression of OSMRβ. Recombinant human OSM strongly activated both STAT1 and STAT3 in HconEpiCs and induced various tissue remodeling-related genes, including MMP-1, MMP-3, IL-24, IL-20, serpinB3, S100A7, tenascin C, and SOCS3.

Conclusion

Our results suggest that OSM is one of the key molecules involved in remodeling of giant papillae in VKC.
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Metadata
Title
Role of oncostatin M in the pathogenesis of vernal keratoconjunctivitis: focus on tissue remodeling
Authors
Keitaro Mashimo
Ayumi Usui-Ouchi
Yousuke Ito
Rei Wakasa-Arai
Norihiko Yokoi
Satoshi Kawasaki
Akira Murakami
Akira Matsuda
Nobuyuki Ebihara
Publication date
01-01-2021
Publisher
Springer Japan
Published in
Japanese Journal of Ophthalmology / Issue 1/2021
Print ISSN: 0021-5155
Electronic ISSN: 1613-2246
DOI
https://doi.org/10.1007/s10384-020-00791-8

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