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Japanese Journal of Ophthalmology
Published in: Japanese Journal of Ophthalmology 4/2019

01-07-2019 | Levofloxacin | Clinical Investigation

Moraxella species: infectious microbes identified by use of time-of-flight mass spectrometry

Authors: Shunsuke Takahashi, Kazuhiro Murata, Kenji Ozawa, Hiroki Yamada, Hideaki Kawakami, Asami Nakayama, Yuko Asano, Kiyofumi Mochizuki, Hiroshige Mikamo

Published in: Japanese Journal of Ophthalmology | Issue 4/2019

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Abstract

Purpose

To report the clinical manifestations, identification, antimicrobial susceptibilities, and treatment outcomes of ocular infections caused by Moraxella species.

Study design

Retrospective study.

Patients and methods

The medical records of all patients treated at the Departments of Ophthalmology of the Ogaki Municipal Hospital and the Gifu University Graduate School of Medicine for ocular infections caused by Moraxella species between January 2011 and June 2017 were examined. The stored Moraxella species isolated from ocular samples were identified by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS), molecular identification, and the biochemical properties.

Results

Sixteen eyes of 16 patients were treated for Moraxella ocular infections. The patients’ median age was 72 years. A predisposing systemic or ocular condition was identified in 15 of the patients. Nine of the patients developed keratitis; four, conjunctivitis; and three, blebitis. M lacunata (6 eyes), M catarrhalis (6), M nonliquefaciens (3), and M osloensis (1) were identified by MALDI-TOF MS. All isolates were sensitive to levofloxacin, tobramycin, ceftazidime, ceftriaxone, and cefazolin. Twelve patients with keratitis or blebitis were treated with various topical antimicrobial combinations, and systemic antibiotics were used in 10 of the 12 patients. The mean time for the complete closure of the epithelial defects with keratitis was 24 days. The visual outcomes after treatment were favorable except in 1 keratitis patient who underwent enucleation.

Conclusions

The use of duo-therapy with a combination of fluoroquinolone and cefmenoxime should be considered in cases nonresponsive to monotherapy, such as keratitis and bleb-associated infections. MALDI-TOF MS is useful for the identification of Moraxella to the species level.
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Metadata
Title
Moraxella species: infectious microbes identified by use of time-of-flight mass spectrometry
Authors
Shunsuke Takahashi
Kazuhiro Murata
Kenji Ozawa
Hiroki Yamada
Hideaki Kawakami
Asami Nakayama
Yuko Asano
Kiyofumi Mochizuki
Hiroshige Mikamo
Publication date
01-07-2019
Publisher
Springer Japan
Published in
Japanese Journal of Ophthalmology / Issue 4/2019
Print ISSN: 0021-5155
Electronic ISSN: 1613-2246
DOI
https://doi.org/10.1007/s10384-019-00669-4

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Correction to: Causes, background, and characteristics of binocular diplopia in the elderly