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European Surgery
Published in: European Surgery 1/2017

Open Access 01-02-2017 | original article

Application of modified small bladder patch-to-bladder double-layer sutures to improve renal transplantation in mice

Authors: Chen Wenwei, Yang Yirong, Katarzyna M. Stevens, Michal Heger, Xia Peng

Published in: European Surgery | Issue 1/2017

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Summary

Background

This study aimed to introduce an improved surgical procedure to reduce the incidence of urinary tract complications after renal transplantation in mice using a modified bladder patch-to-bladder anastomosis technique.

Methods

Renal isotransplantation was performed in 28 male C57BL/6 mice. The urinary tract was reconstructed with a ureteral anastomosis between the donor’s small bladder patch and the recipient’s bladder. The bladder patch was secured through a cystotomy in the recipient’s bladder mucosa and seromuscular layers, which were sutured in a double-layer manner. The food intake and survival of mice were recorded for 100 days in addition to monitoring appearance, weight, and symptoms of pain. On post-transplantation day 7, the native kidney in the recipients was removed and the transplanted kidney assessed visually. Urine leakage from the transplanted graft was monitored by assessing the degree of ascites.

Results

The success rate of renal transplantation was 82 % (23 of 28 cases). Arterial thrombosis at the site of anastomosis occurred in 3 cases (11 %) and hemorrhagic shock in 2 cases (7 %). The mean ± SD time of the operation in recipients was 81 ± 5 min. No complications were noted in the successfully transplanted animals.

Conclusions

The modified procedure of a small bladder patch-to-bladder with double-layer suturing minimizes complications after renal transplantation in mice while requiring the same operating time as other approaches such as ureter to bladder anastomosis, which are associated with more complications.
Literature
1.
Skoskiewicz M, Chase C, Winn HJ, Russell PS. Kidney transplants between mice of graded immunogenetic diversity. Transplant Proc. 1973;5:721–725.PubMed
2.
Kalina SL, Mottram PL. A microsurgical technique for renal transplantation in mice. Aust N Z J Surg. 1993;63:213–216.CrossRefPubMed
3.
Zhang Z, Schlachta C, Duff J, Stiller C, Grant D, Zhong R. Improved techniques for kidney transplantation in mice. Microsurgery. 1995;16:103–109.CrossRefPubMed
4.
Lau A, Khan K, Pavlosky A, Yin Z, Huang X, Haig A, Liu W, Singh B, Zhang ZX, Jevnikar AM. Serine protease inhibitor-6 inhibits granzyme B‑mediated injury of renal tubular cells and promotes renal allograft survival. Transplantation. 2014;98:402–410.CrossRefPubMed
5.
Franceschini N, Cheng O, Zhang X, Ruiz P, Mannon RB. Inhibition of prolyl-4-hydroxylase ameliorates chronic rejection of mouse kidney allografts. Am J Transplant. 2003;3:396–402.CrossRefPubMed
6.
Ross R. Genetically modified mice as models of transplant atherosclerosis. Nat Med. 1996;2:527–528.CrossRefPubMed
7.
Nesbitt T, Coffman TM, Griffiths R, Drezner MK. Crosstransplantation of kidneys in normal and Hyp mice. Evidence that the Hyp mouse phenotype is unrelated to an intrinsic renal defect. J Clin Invest. 1992;89:1453–1459.CrossRefPubMedPubMedCentral
8.
Meng L, Wu Z, Wang Y, Lassman C, Busuttil RW, Zhai Y, Kupiec-Weglinski JW. Differential impact of CD154 costimulation blockade on alloreactive effector and regulatory T cells in murine renal transplant recipients. Transplantation. 2008;85:1332–1338.CrossRefPubMed
9.
Mannon RB, Doyle C, Griffiths R, Bustos M, Platt JL, Coffman TM. Altered intragraft immune responses and improved renal function in MHC class II-deficient mouse kidney allografts. Transplantation. 2000;69:2137–2143.CrossRefPubMed
10.
Ziegler E, Gueler F, Rong S, Mengel M, Witzke O, Kribben A, Haller H, Kunzendorf U, Krautwald S. CCL19-IgG prevents allograft rejection by impairment of immune cell trafficking. J Am Soc Nephrol. 2006;17:2521–2532.CrossRefPubMed
11.
Coffman T, Geier S, Ibrahim S, Griffiths R, Spurney R, Smithies O, Koller B, Sanfilippo F. Improved renal function in mouse kidney allografts lacking MHC class I antigens. J Immunol. 1993;151:425–435.PubMed
12.
Russell PS, Chase CM, Colvin RB, Plate JM. Kidney transplants in mice. An analysis of the immune status of mice bearing long-term, H‑2 incompatible transplants. J Exp Med. 1978;147:1449–1468.CrossRefPubMedPubMedCentral
13.
Qi F, Adair A, Ferenbach D, Vass DG, Mylonas KJ, Kipari T, Clay M, Kluth DC, Hughes J, Marson LP. Depletion of cells of monocyte lineage prevents loss of renal microvasculature in murine kidney transplantation. Transplantation. 2008;86:1267–1274.CrossRefPubMed
14.
Gachelin G, Dumas B, Abastado JP, Cami B, Papamatheakis J, Kourilsky P. Mouse genes coding for the major class I transplantation antigens: a mosaic structure might be related to the antigenic polymorphism. Ann Immunol (Paris). 1982;133C:3–20.
15.
Famulski KS, Einecke G, Reeve J, Ramassar V, Allanach K, Mueller T, Hidalgo LG, Zhu LF, Halloran PF. Changes in the transcriptome in allograft rejection: IFN-gamma-induced transcripts in mouse kidney allografts. Am J Transplant. 2006;6:1342–1354.CrossRefPubMed
16.
Erickson RP. Mouse models of human genetic disease: which mouse is more like a man? Bioessays. 1996;18:993–998.CrossRefPubMed
17.
Mak TW, Penninger JM, Ohashi PS. Knockout mice: a paradigm shift in modern immunology. Nat Rev Immunol. 2001;1:11–19.CrossRefPubMed
18.
Martins PN. Learning curve, surgical results and operative complications for kidney transplantation in mice. Microsurgery. 2006;26:590–593.CrossRefPubMed
19.
Ge F, Gong W. Strategies for successfully establishing a kidney transplant in a mouse model. Exp Clin Transplant. 2011;9:287–294.PubMed
20.
Martins PN. Technique of kidney transplantation in mice with anti-reflux urinary reconstruction. Int Braz J Urol. 2006;32:713–718.CrossRefPubMed
21.
Gargiulo S, Greco A, Gramanzini M, Esposito S, Affuso A, Brunetti A, Vesce G. Mice anesthesia, analgesia, and care, Part I: anesthetic considerations in preclinical research. ILAR J. 2012;53:E55–E69.CrossRefPubMed
22.
Arras M, Rettich A, Cinelli P, Kasermann HP, Burki K. Assessment of post-laparotomy pain in laboratory mice by telemetric recording of heart rate and heart rate variability. BMC Vet Res. 2007;3:16.CrossRefPubMedPubMedCentral
23.
Hawkins P. Recognizing and assessing pain, suffering and distress in laboratory animals: a survey of current practice in the UK with recommendations. Lab Anim. 2002;36:378–395.CrossRefPubMed
24.
Grigoryev DN, Liu M, Cheadle C, Barnes KC, Rabb H. Genomic profiling of kidney ischemia-reperfusion reveals expression of specific alloimmunity-associated genes: Linking “immune” and “nonimmune” injury events. Transplant Proc. 2006;38:3333–3336.CrossRefPubMedPubMedCentral
25.
Wang M, Bai J, Baumann M, Heemann U. New model for simultaneous heart and kidney transplantation in mice. Microsurgery. 2003;23:164–168.CrossRefPubMed
26.
Kinnaert P, Hall M, Janssen F, Vereerstraeten P, Toussaint C, Van GJ. Ureteral stenosis after kidney transplantation: true incidence and long-term followup after surgical correction. J Urol. 1985;133:17–20.PubMed
27.
Shoskes DA, Hanbury D, Cranston D, Morris PJ. Urological complications in 1,000 consecutive renal transplant recipients. J Urol. 1995;153:18–21.CrossRefPubMed
28.
Faenza A, Nardo B, Catena F, Scolari MP, d’Arcangelo GL, Buscaroli A, Rossi C, Zompatori M. Ureteral stenosis after kidney transplantation. A study on 869 consecutive transplants. Transpl Int. 1999;12:334–340.PubMed
Metadata
Title
Application of modified small bladder patch-to-bladder double-layer sutures to improve renal transplantation in mice
Authors
Chen Wenwei
Yang Yirong
Katarzyna M. Stevens
Michal Heger
Xia Peng
Publication date
01-02-2017
Publisher
Springer Vienna
Published in
European Surgery / Issue 1/2017
Print ISSN: 1682-8631
Electronic ISSN: 1682-4016
DOI
https://doi.org/10.1007/s10353-016-0391-7

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