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Magnetic Resonance Materials in Physics, Biology and Medicine
Published in: Magnetic Resonance Materials in Physics, Biology and Medicine 2/2019

Open Access 01-04-2019 | Research Article

CDP-choline accumulation in breast and colorectal cancer cells treated with a GSK-3-targeting inhibitor

Authors: Su Myat Phyu, Chih-Chung Tseng, Tim Andrew Davies Smith

Published in: Magnetic Resonance Materials in Physics, Biology and Medicine | Issue 2/2019

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Abstract

Purpose

Glycogen synthase kinase 3 (GSK3) is a key controlling element of many cellular processes including cell-cycle progression and recent studies suggest that GSK3 is a potential anticancer target. Changes in glucose metabolism associated with GSK3 inhibition may impact on lipid synthesis, whilst lipid metabolites can act as molecular response markers.

Methods

Here, SKBr3 breast and HCT8 colorectal cancer cells were treated with the GSK3 inhibitor SB216763, and [14C (U)] glucose and [3H] choline incorporation into lipids was determined. Cell extracts from treated cells were subject to 31P NMR spectroscopy.

Results

SB216763 treatment decreased choline incorporation into lipids and caused an accumulation of CDP-choline which was accompanied by decreased conversion of glucose into lipid components.

Conclusion

SB216763 profoundly inhibits phospholipid synthesis in cancer cells which demonstrate accumulation of CDP-choline detectable by 31P NMR spectroscopy. Metabolic changes in lipid metabolism present potential response markers to drugs targeting GSK3.
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Metadata
Title
CDP-choline accumulation in breast and colorectal cancer cells treated with a GSK-3-targeting inhibitor
Authors
Su Myat Phyu
Chih-Chung Tseng
Tim Andrew Davies Smith
Publication date
01-04-2019
Publisher
Springer International Publishing
Published in
Magnetic Resonance Materials in Physics, Biology and Medicine / Issue 2/2019
Print ISSN: 0968-5243
Electronic ISSN: 1352-8661
DOI
https://doi.org/10.1007/s10334-018-0719-3

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