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Magnetic Resonance Materials in Physics, Biology and Medicine
Published in: Magnetic Resonance Materials in Physics, Biology and Medicine 6/2016

Open Access 01-12-2016 | Research Article

MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis

Authors: Katarzyna Byk, Krzysztof Jasinski, Zaneta Bartel, Agnieszka Jasztal, Barbara Sitek, Boguslaw Tomanek, Stefan Chlopicki, Tomasz Skorka

Published in: Magnetic Resonance Materials in Physics, Biology and Medicine | Issue 6/2016

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Abstract

Objective

To assess alterations in perfusion and liver function in the concanavalin A (ConA)-induced mouse model of acute liver failure (ALF) using two magnetic resonance imaging (MRI)-based methods: dynamic contrast-enhanced MRI (DCE-MRI) with Gd-EOB-DTPA contrast agent and arterial spin labelling (ASL).

Materials and methods

BALB/c mice were studied using a 9.4 T MRI system. The IntraGateFLASHTM and FAIR-EPI pulse sequences were used for optimum mouse abdomen imaging.

Results

The average perfusion values for the liver of the control and ConA group were equal to 245 ± 20 and 200 ± 32 ml/min/100 g (p = 0.008, respectively). DCE-MRI showed that the time to the peak of the image enhancement was 6.14 ± 1.07 min and 9.72 ± 1.69 min in the control and ConA group (p < 0.001, respectively), while the rate of the contrast wash-out in the control and ConA group was 0.037 ± 0.008 and 0.021 ± 0.008 min−1 (p = 0.004, respectively). These results were consistent with hepatocyte injury in the ConA-treated mice as confirmed by histopathological staining.

Conclusions

Both the ASL and DCE-MRI techniques represent a reliable methodology to assess alterations in liver perfusion and hepatocyte integrity in murine hepatitis.
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Metadata
Title
MRI-based assessment of liver perfusion and hepatocyte injury in the murine model of acute hepatitis
Authors
Katarzyna Byk
Krzysztof Jasinski
Zaneta Bartel
Agnieszka Jasztal
Barbara Sitek
Boguslaw Tomanek
Stefan Chlopicki
Tomasz Skorka
Publication date
01-12-2016
Publisher
Springer Berlin Heidelberg
Published in
Magnetic Resonance Materials in Physics, Biology and Medicine / Issue 6/2016
Print ISSN: 0968-5243
Electronic ISSN: 1352-8661
DOI
https://doi.org/10.1007/s10334-016-0563-2

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