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21-11-2022 | Candida Balanitis | Original Article

Evaluation of efficacy of new chalcone-based endodontic irrigant against dual biofilm Enterococcus faecalis and Candida albicans: a study in vitro

Authors: Joice Graciani, Pedro Luiz Rosalen, Emmanuely de Oliveira Chaves dos Santos, Kellyth Andrade Pereira Rocha, Bruno Rafael Teixeira Balen, Mayara Aparecida Rocha Garcia, Josy Goldoni Lazarini, Diego Romario da Silva, Suzana Gonçalves Carvalho, Luís Octávio Regasini, Marlus Chorilli, Janaina de Cássia Orlandi Sardi

Published in: Odontology | Issue 3/2023

Abstract

The aim of this research was to develop a chalcone-based endodontic irrigant for cleaning and disinfecting the root canal. Minimal inhibitory concentration (MIC) experiments in C. albicans and E. faecalis strains with different aminochalcones (AM) were carried out, and the compound that presented the best activity against both pathogens was chosen. The formulation of an endodontic irrigant was elaborated, tested in mono and dual specie biofilms. Disks were sterilized and then incubated with E. faecalis, C. albicans and E. faecalis and C. albicans mixed for 72 h for biofilm maturation. After contamination, samples were divided in 4 experimental groups and 2 positive control group as follows: Group1: Irrigant; Group2: Irrigant + AM-38; Group3: Chlorhexidine 2% (positive control) and, Group 4: 1.0% sodium hypochlorite (positive control). The samples were analyzed by CFU/ml count. The sample was taken to sonicador to remove the cells and then plated. The toxicity was determined in vitro with human gingival fibroblast cells (HGF) and in vivo using the Galleria mellonella model. Formulation showed antimicrobial activity, with MIC on C. albicans 15.6 and E. faecalis 7.8 µg/ml. Treatment with formulation in concentration 156 µg/ml significantly reduced mono or dual species biofilm formation and viability (p < 0.05). The results were significant against C. albicans and E. faecalis and did not show toxicity in cells and G. mellonella. In general, the formulation showed effective antibiofilm activity, significantly reducing microorganisms, opening paths in search of new endodontic irrigants.

Cardoso MG, de Oliveira LD, Koga-Ito CY, Jorge AO. Effectiveness of ozonated water on Candida albicans, Enterococcus faecalis, and endotoxins in root canals. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2008;105(3):e85–91. https://doi.org/10.1016/j.tripleo.2007.10.006.CrossRefPubMed

Dornelles-Morgental R, Guerreiro-Tanomaru JM, de Faria-Júnior NB, Hungaro-Duarte MA, Kuga MC, Tanomaru-Filho M. Antibacterial efficacy of endodontic irrigating solutions and their combinations in root canals contaminated with Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2011;112(3):396–400. https://doi.org/10.1016/j.tripleo.2011.02.004.CrossRefPubMed

Bernardi S, Anderson A, Macchiarelli G, Hellwig E, Cieplik F, Vach K, Al-Ahmad A. Subinhibitory antibiotic concentrations enhance biofilm formation of clinical Enterococcus faecalis Isolates. Antibiotics. 2021;10(7):874. https://doi.org/10.3390/antibiotics10070874.CrossRefPubMedPubMedCentral

Alghamdi F, Shakir M. The influence of enterococcus faecalis as a dental root canal pathogen on endodontic treatment: a systematic review. Cureus. 2020;12(3): e7257. https://doi.org/10.7759/cureus.7257.CrossRefPubMedPubMedCentral

Yoo YJ, Kim AR, Perinpanayagam H, Han SH, Kum KY. Candida albicans virulence factors and pathogenicity for endodontic infections. Microorganisms. 2020;8(9):1300. https://doi.org/10.3390/microorganisms8091300.CrossRefPubMedPubMedCentral

Ndiaye D, Diongue K, Bane K, Niang SO, Ndiaye D, Touré B. Recherche de levures dans les primo-infections endodontiques et étude de leur sensibilité à une désinfection à l’hypochlorite de sodium à 2,5 [Presence of yeasts in primary endodontic infections and study of their susceptibility to disinfection with sodium hypochlorite at 2.5]. J Mycol Med. 2016;26(2):111–5. https://doi.org/10.1016/j.mycmed.2016.01.001.CrossRefPubMed

Mergoni G, Percudani D, Lodi G, Bertani P, Manfredi M. Prevalence of candida species in endodontic infections: systematic review and meta-analysis. J Endod. 2018;44(11):1616-1625.e9. https://doi.org/10.1016/j.joen.2018.07.016.CrossRefPubMed

Du Q, Yuan S, Zhao S, Fu D, Chen Y, Zhou Y, Cao Y, Gao Y, Xu X, Zhou X, He J. Coexistence of Candida albicans and Enterococcus faecalis increases biofilm virulence and periapical lesions in rats. Biofouling. 2021;37(9–10):964–74. https://doi.org/10.1080/08927014.2021.1993836.CrossRefPubMed

Richards D, Davies JK, Figdor D. Starvation survival and recovery in serum of Candida albicans compared with Enterococcus faecalis. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;110(1):125–30. https://doi.org/10.1016/j.tripleo.2010.03.007.CrossRefPubMed

10.

Xu M, Wu P, Shen F, Ji J, Rakesh KP. Chalcone derivatives and their antibacterial activities: current development. Bioorg Chem. 2019;91: 103133. https://doi.org/10.1016/j.bioorg.2019.103133.CrossRefPubMed

11.

Zhuang C, Zhang W, Sheng C, Zhang W, Xing C, Miao Z. Chalcone: a privileged structure in medicinal chemistry. Chem Rev. 2017;117(12):7762–810. https://doi.org/10.1021/acs.chemrev.7b00020.CrossRefPubMedPubMedCentral

12.

Bila NM, Costa-Orlandi CB, Vaso CO, Bonatti JLC, de Assis LR, Regasini LO, Fontana CR, Fusco-Almeida AM, Mendes-Giannini MJS. 2-Hydroxychalcone as a potent compound and photosensitizer against dermatophyte biofilms. Front Cell Infect Microbiol. 2021;13(11): 679470. https://doi.org/10.3389/fcimb.2021.679470.CrossRef

13.

Medina-Alarcón KP, Singulani LJ, Dutra LA, Pitangui SN, Pereira-da-Silva MA, Dos Santos MB, Ayusso GM, Regasini LO, Soares CP, Chorilli M, Mendes-Giannini MJ, Fusco-Almeida AM. Antifungal activity of 2’-hydroxychalcone loaded in nanoemulsion against Paracoccidioides spp. Future Microbiol. 2020;15:21–33. https://doi.org/10.2217/fmb-2019-0095.CrossRefPubMed

14.

Marques BC, Santos MB, Anselmo DB, Monteiro DA, Gomes E, Saiki MFC, Rahal P, Rosalen PL, Sardi JCO, Regasini LO. Methoxychalcones: effect of methoxyl group on the antifungal antibacterial and antiproliferative activities. Med Chem. 2020;16(7):881–91. https://doi.org/10.2174/1573406415666190724145158.CrossRefPubMed

15.

Emeri FTAS, Rosalen PL, Paganini ÉR, Garcia MAR, Nazaré AC, Lazarini JG, Alencar SM, Regasini LO, Sardi JCO. Antimicrobial activity of nitrochalcone and pentyl caffeate against hospital pathogens results in decreased microbial adhesion and biofilm formation. Biofouling. 2019;35(2):129–42. https://doi.org/10.1080/08927014.2019.1574763.CrossRefPubMed

16.

Bombarda GF, Rosalen PL, Paganini ER, Garcia MA, Silva DR, Lazarini JG, Freires IA, Regasini LO, Sardi JC. Bioactive molecule optimized for biofilm reduction related to childhood caries. Future Microbiol. 2019;14:1207–20. https://doi.org/10.2217/fmb-2019-0144.CrossRefPubMed

17.

Insuasty B, Ramírez J, Becerra D, Echeverry C, Quiroga J, Abonia R, Robledo SM, Vélez ID, Upegui Y, Muñoz JA, Ospina V, Nogueras M, Cobo J. An efficient synthesis of new caffeine-based chalcones, pyrazolines and pyrazolo[3,4-b][1,4]diazepines as potential antimalarial, antitrypanosomal and antileishmanial agents. Eur J Med Chem. 2015;26(93):401–13. https://doi.org/10.1016/j.ejmech.2015.02.040.CrossRef

18.

Liu Z, Tang L, Zou P, Zhang Y, Wang Z, Fang Q, Jiang L, Chen G, Xu Z, Zhang H, Liang G. Synthesis and biological evaluation of allylated and prenylated mono-carbonyl analogs of curcumin as anti-inflammatory agents. Eur J Med Chem. 2014;3(74):671–82. https://doi.org/10.1016/j.ejmech.2013.10.061.CrossRef

19.

Garcia M, Theodoro RS, Sardi J, Santos MB, Ayusso GM, Pavan FR, Costa AR, Santa Cruz LM, Rosalen PL, Regasini LO. Design, synthesis and antibacterial activity of chalcones against MSSA and MRSA planktonic cells and biofilms. Bioorg Chem. 2021;116: 105279. https://doi.org/10.1016/j.bioorg.2021.105279.CrossRefPubMed

20.

CLSI. Reference method for broth dilution antifungal susceptibility testing of yeasts; approved standard. CLSI document M27–A3. 3rd ed. Wayne: Clinical and Laboratory Standards Institute; 2008. (ISBN-1-56238-666-2).

21.

CLSI. Reference method for broth dilution antifungal susceptibility testing of bacterial; approved standard. CLSI document M07–A9. 9th ed. Wayne: Clinical and Laboratory Standards Institute; 2012. (ISBN 1-56238-783-9).

22.

Lamfon H, Porter SR, McCullough M, Pratten J. Formation of Candida albicans biofilms on non-shedding oral surfaces. Eur J Oral Sci. 2003;111(6):465–71. https://doi.org/10.1111/j.0909-8836.2003.00084.x.CrossRefPubMed

23.

Sardi JCO, Polaquini CR, Freires IA, Galvão LCC, Lazarini JG, Torrezan GS, Regasini LO, Rosalen PL. Antibacterial activity of diacetylcurcumin against Staphylococcus aureus results in decreased biofilm and cellular adhesion. J Med Microbiol. 2017;66(6):816–24. https://doi.org/10.1099/jmm.0.000494.CrossRefPubMed

24.

Zancan RF, Calefi PHS, Borges MMB, Lopes MRM, de Andrade FB, Vivan RR, Duarte MAH. Antimicrobial activity of intracanal medications against both Enterococcus faecalis and Candida albicans biofilm. Microsc Res Tech. 2019;82(5):494–500. https://doi.org/10.1002/jemt.23192.CrossRefPubMed

25.

Kovac J, Kovac D, Slobodnikova L, Kotulova D. Enterococcus faecalis and Candida albicans in the dental root canal and periapical infections. Bratisl Lek Listy. 2013;114(12):716–20.PubMed

26.

Faria G, Cardoso CR, Larson RE, Silva JS, Rossi MA. Chlorhexidine-induced apoptosis or necrosis in L929 fibroblasts: a role for endoplasmic reticulum stress. Toxicol Appl Pharmacol. 2009;234(2):256–65. https://doi.org/10.1016/j.taap.2008.10.012.CrossRefPubMed

27.

Mohammadi Z, Abbott PV. The properties and applications of chlorhexidine in endodontics. Int Endod J. 2009;42(4):288–302. https://doi.org/10.1111/j.1365-2591.2008.01540.x.CrossRefPubMed

28.

Cabral CT, Fernandes MH. In vitro comparison of chlorhexidine and povidone-iodine on the long-term proliferation and functional activity of human alveolar bone cells. Clin Oral Investig. 2007;11(2):155–64. https://doi.org/10.1007/s00784-006-0094-8.CrossRefPubMed

29.

Kozol RA, Gillies C, Elgebaly SA. Effects of sodium hypochlorite (Dakin’s solution) on cells of the wound module. Arch Surg. 1988;123(4):420–3. https://doi.org/10.1001/archsurg.1988.01400280026004.CrossRefPubMed

Title: Evaluation of efficacy of new chalcone-based endodontic irrigant against dual biofilm Enterococcus faecalis and Candida albicans: a study in vitro
Authors: Joice Graciani
Pedro Luiz Rosalen
Emmanuely de Oliveira Chaves dos Santos
Kellyth Andrade Pereira Rocha
Bruno Rafael Teixeira Balen
Mayara Aparecida Rocha Garcia
Josy Goldoni Lazarini
Diego Romario da Silva
Suzana Gonçalves Carvalho
Luís Octávio Regasini
Marlus Chorilli
Janaina de Cássia Orlandi Sardi
Publication date: 21-11-2022
Publisher: Springer Nature Singapore
Keywords: Candida Balanitis
Candida Albicans
Published in: Odontology / Issue 3/2023
Print ISSN: 1618-1247
Electronic ISSN: 1618-1255
DOI: https://doi.org/10.1007/s10266-022-00764-y

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Evaluation of efficacy of new chalcone-based endodontic irrigant against dual biofilm Enterococcus faecalis and Candida albicans: a study in vitro

Abstract

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Abstract

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