Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress 2023 EHA Congress 2023 ASCO Annual Meeting
Clinical Collections
Advances in Alzheimer’s

At a glance: The ONWARDS insulin icodec trials

A round-up of the ONWARDS phase 3 clinical trials evaluating the novel weekly insulin icodec in people with diabetes.
ADVANCED SEARCH
Log in
Top
Odontology
Published in: Odontology 3/2018

01-07-2018 | Original Article

Clinical usability of aspartate aminotransferase to evaluate the prognosis of periodontal regeneration therapies: prospective, longitudinal study

Authors: Yohei Nakayama, Miyuki Takei-Obi, Izumi Toyoshima-Matsumura, Mai Tsutamori, Ayako Kato, Chiharu Okano, Masaru Mezawa, Yorimasa Ogata

Published in: Odontology | Issue 3/2018

Login to get access

Abstract

To evaluate the degree of periodontal tissue destruction, aspartate aminotransferase (AST) levels in the gingival crevicular fluid (GCF) are utilized as a predictor of periodontal therapy. We have previously shown that the usefulness of AST activities [periodontal tissue monitor (PTM) values] using a PTM-kit to evaluate the effects of initial periodontal therapy and periodontal regeneration therapy by enamel matrix derivative (EMD). This prospective, longitudinal study was conducted using 38 healthy and 80 periodontitis sites with probing depth (PD) of 5–10 mm for guided tissue regeneration (GTR) and EMD from 36 patients. GCF samples were used to evaluate PTM values at base line (BL) and after 6 months of surgeries (re-evaluation: RE), and periodontal examinations were performed concurrently. PTM values at BL were statistically improved at RE, accompanied by the improvement of periodontal parameters in both groups. PTM values and PD, and the clinical attachment level (CAL) showed high correlations. PD, CAL and bleeding on probing (BOP) were highly correlated with PTM values in both groups, whereas only PD showed a significant correlation with PTM values at RE in the GTR group. Change in the amounts of PD, CAL and BOP between BL and RE in both groups showed no correlation with PTM values. In the negative PTM value sites at BL in EMD group, the mean PD was significantly reduced at RE compared with positive PTM sites at BL. PTM values are able to be utilized as the biochemical predictor of prognosis after periodontal regeneration therapy.
Literature
1.
Smith AJ, Alexander M, Mackenzie D, Lennon A, Riggio MP, MacFarlane TW. Microbial factors and gingival crevicular fluid aspartate aminotransferase levels. A cross-sectional study. J Clin Periodontol. 1998;25:334–9.CrossRefPubMed
2.
Huynh C, Roch-Arveiller M, Meyer J, Giroud JP. Gingival crevicular fluid of patients with gingivitis or periodontal disease: evaluation of elastase-alpha 1 proteinase inhibitor complex. J Clin Periodontol. 1992;19:187–92.CrossRefPubMed
3.
Imamura K, Takayama S, Saito A, Inoue E, Nakayama Y, Ogata Y, Shirakawa S, Nagano T, Gomi K, Morozumi T, Akiishi K, Watanabe K, Yoshie H. Evaluation of a novel immunochromatographic device for rapid and accurate clinical detection of Porphyromonas gingivalis in subgingival plaque. J Microbiological Methods. 2015;117:4–10.CrossRef
4.
Seymour GJ, Gemmell E, Reinhardt RA, Eastcott J, Taubman MA. Immunopathogenesis of chronic inflammatory periodontal disease: cellular and molecular mechanisms. J Periodontal Res. 1993;28:478–86.CrossRefPubMed
5.
Socransky SS, Haffajee AD, Goodson JM, Lindhe J. New concepts of destructive periodontal disease. J Clin Periodontol. 1984;11:21–32.CrossRefPubMed
6.
Chambers DA, Crawford JM, Mukherjee S, Cohen RL. Aspartate aminotransferase increases in crevicular fluid during experimental periodontitis in beagle dogs. J Periodontol. 1984;55:526–30.CrossRefPubMed
7.
Persson GR, DeRouen TA, Page RC. Relationship between levels of aspartate aminotransferase in gingival crevicular fluid and gingival inflammation. J Periodontal Res. 1990;25:17–24.CrossRefPubMed
8.
Chambers DA, Imrey PB, Cohen RL, Crawford JM, Alves ME, McSwiggin TA. A longitudinal study of aspartate aminotransferase in human gingival crevicular fluid. J Periodontal Res. 1991;26:65–74.CrossRefPubMed
9.
Imrey PB, Crawford JM, Cohen RL, Alves ME, McSwiggin TA, Chambers DA. A cross-sectional analysis of aspartate aminotransferase in human gingival crevicular fluid. J Periodontal Res. 1991;26:75–84.CrossRefPubMed
10.
McCulloch CA. Host enzymes in gingival crevicular fluid as diagnostic indicators of periodontitis. J Clin Periodontol. 1994;21:497–506.CrossRefPubMed
11.
Ivić-Kardum M, Aurer A, Haban V, Aurer-Kozelj J, Szirovicza L. Aspartat aminotransferase-a marker of periodontal disease activity. Coll Antropol. 1999;23:111–6.PubMed
12.
Shimada K, Mizuno T, Uchida T, Kato T, Ito K, Murai S. Relationship between levels of aspartate aminotransferase in gingival crevicular fluid and conventional measures of periodontal status assessed using PocketWatch: a cross-sectional study. J Oral Sci. 1999;41:35–40.CrossRefPubMed
13.
Tsalikis L, Malaka E, Pavlitou E, Konstantinidis A. Aspartate aminotransferase levels in gingival crevicular fluid before and after initial periodontal treatment. J Int Acad Periodontol. 2001;3:68–74.PubMed
14.
Wong MY, Lu CL, Liu CM, Hou LT, Chang W. Clinical response of localized recurrent periodontitis treated with scaling, root planing, and tetracycline fiber. J Formos Med Assoc. 1998;97:490–7.PubMed
15.
Knöfler G, Purschwitz R, Jentsch H, Birkenmeier G, Schmidt H. Gingival crevicular fluid levels of aspartate aminotransferase and alpha 2-macroglobulin before and after topical application of metronidazole or scaling and root planing. Quintessence Int. 2008;39:381–9.PubMed
16.
Shimada K, Mizuno T, Ohshio K, Kamaga M, Murai S, Ito K. Analysis of aspartate aminotransferase in gingival crevicular fluid assessed by using PocketWatch: a longitudinal study with initial therapy. J Clin Periodontol. 2000;11:819–23.CrossRef
17.
Takei M, Nakayama Y, Toyoshima I, Hiromatsu Y, Ogata Y. Comparison of the levels of aspartate aminotransferase and clinical parameters pre- and post- initial therapy and periodontal surgery. J Jpn Soc Periodontal. 2014;56:390–8.CrossRef
18.
Oringer RJ, Howell TH, Nevins ML, Reasner DS, Davis GH, Sekler J, Fiorellini JP. Relationship between crevicular aspartate aminotransferase levels and periodontal disease progression. J Periodontol. 2001;72:17–24.CrossRefPubMed
19.
Glavind L, Löe H. Errors in the clinical assessment of periodontal destruction. J Periodontal Res. 1967;23:180–4.CrossRef
20.
Kamma JJ, Nakou M, Persson RG. Association of early onset periodontitis microbiota with aspartate aminotransferase activity in gingival crevicular fluid. J Clin Periodontol. 2001;28:1096–105.CrossRefPubMed
21.
Sculean A, Donos N, Windisch P, Brecx M, Gera I, Reich E, Karring T. Healing of human intrabony defects following treatment with enamel matrix proteins or guided tissue regeneration. J Periodontal Res. 1999;34:310–22.CrossRefPubMed
22.
Cortellini P, Prato GP, Tonetti MS. The modified papilla preservation technique. A new surgical approach for interproximal regenerative procedures. J Periodontol. 1995;66:261–6.CrossRefPubMed
23.
Cortellini P, Prato GP, Tonetti MS. The simplified papilla preservation flap. A novel surgical approach for the management of soft tissues in regenerative procedures. Int J Periodontics Restor Dent. 1999;19:589–99.
24.
Cortellini P, Tonetti MS, Lang NP, Suvan JE, Zucchelli G, Vangsted T, Silvestri M, Rossi R, McClain P, Fonzar A, Dubravec D, Adriaens P. The simplified papilla preservation flap in the regenerative treatment of deep intrabony defects: clinical outcomes and postoperative morbidity. J Periodontol. 2001;72:1702–12.CrossRefPubMed
25.
Guida L, Annunziata M, Belardo S, Farina R, Scabbia A, Trombelli L. Effect of autogenous cortical bone particulate in conjunction with enamel matrix derivative in the treatment of periodontal intraosseous defects. J Periodontol. 2007;78:231–8.CrossRefPubMed
26.
Yilmaz S, Cakar G, Yildirim B, Sculean A. Healing of two and three wall intrabony periodontal defects following treatment with an enamel matrix derivative combined with autogenous bone. J Clin Periodontol. 2010;37:544–50.CrossRefPubMed
27.
De Leonardis D, Paolantonio M. Enamel matrix derivative, alone or associated with a synthetic bone substitute, in the treatment of 1- to 2-wall periodontal defects. J Periodontol. 2013;84:444–55.CrossRefPubMed
28.
Tonetti MS, Lang NP, Cortellini P, Suvan JE, Adriaens P, Dubravec D, Fonzar A, Fourmousis I, Mayfield L, Rossi R, Silvestri M, Tiedemann C, Topoll H, Vangsted T, Wallkamm B. Enamel matrix proteins in the regenerative therapy of deep intrabony defects. J Clin Periodontol. 2002;29:317–25.CrossRefPubMed
29.
Siciliano VI, Andreuccetti G, Siciliano AI, Blasi A, Sculean A, Salvi GE. Clinical outcomes after treatment of non-contained intrabony defects with enamel matrix derivative or guided tissue regeneration: a 12-month randomized controlled clinical trial. J Periodontol. 2011;82:62–71.CrossRefPubMed
30.
Wei FL, Wang CL, Liu DX, Guo J, Ke HF, Guo XX. Changes of aspartate aminotransferase and alkaline phosphatase activities in gingival crevicular fluid during rapid palatal expansion. Shanghai Kou Qiang Yi Xue. 2007;16:168–71 (in Chinese).PubMed
31.
Wong MY, Lu CL, Liu CM, Hou LT, Chang WK. Clinical response of localized recurrent periodontitis treated with scaling, root planing, and tetracycline fiber. J Formos Med Assoc. 1998;97:490–7.PubMed
32.
Nowzari H, Matian F, Slots J. Periodontal pathogens on polytetrafluoroethylene membrane for guided tissue regeneration inhibits healing. J Clin Periodontol. 1998;22:469–74.CrossRef
33.
Vergara JA, Quiñones CR, Nasjleti CE, Caffesse RG. Vascular response to guided tissue regeneration procedures using nonresorbable and bioabsorbable membranes in dogs. J Periodontol. 1997;68:217–24.CrossRefPubMed
34.
Sakoda K, Nakajima Y, Noguchi K. Enamel matrix derivative induces production of vascular endothelial cell growth factor in human gingival fibroblasts. Eur J Oral Sci. 2012;6:513–9.CrossRef
35.
Bertl K, An N, Bruckmann C, Dard M, Andrukhov O, Matejka M, Rausch-Fan X. Effects of enamel matrix derivative on proliferation/viability, migration, and expression of angiogenic factor and adhesion molecules in endothelial cells in vitro. J Periodontol. 2009;80:1622–30.CrossRefPubMed
36.
Kasaj A, Meister J, Lehmann K, Stratul SI, Schlee M, Stein JM, Willershausen B, Schmidt M. The influence of enamel matrix derivative on the angiogenic activity of primary endothelial cells. J Periodontal Res. 2012;47:479–87.CrossRefPubMed
37.
Lei Q, Chen J, Jiang J, Fu X, Lin H, Cai Z. Comparison of soft tissue healing around implants in beagle dogs: flap surgery versus flapless surgery. Oral Surg Oral Med Oral Pathol Oral Radiol. 2013;115:e21–7.CrossRefPubMed
38.
Lossdorfer S, Sun M, Gotz W, Dard M, Jager A. Enamel matrix derivative promotes human periodontal ligament cell differentiation and osteoprotegerin production in vitro. J Dent Res. 2007;86:980–5.CrossRefPubMed
39.
Miron RJ, Dard M, Weinreb M. Enamel matrix derivative, inflammation and soft tissue wound healing. J Periodont Res. 2015;50:555–69.CrossRefPubMed
Metadata
Title
Clinical usability of aspartate aminotransferase to evaluate the prognosis of periodontal regeneration therapies: prospective, longitudinal study
Authors
Yohei Nakayama
Miyuki Takei-Obi
Izumi Toyoshima-Matsumura
Mai Tsutamori
Ayako Kato
Chiharu Okano
Masaru Mezawa
Yorimasa Ogata
Publication date
01-07-2018
Publisher
Springer Japan
Published in
Odontology / Issue 3/2018
Print ISSN: 1618-1247
Electronic ISSN: 1618-1255
DOI
https://doi.org/10.1007/s10266-017-0328-z

Other articles of this Issue 3/2018

Odontology 3/2018 Go to the issue

Original Article

Effect of root canal sealers on human periodontal ligament fibroblast viability: ex vivo study

Original Article

Open flap versus flapless placement of dental implants. A randomized controlled pilot trial

Original Article

Effect of Er:YAG laser enamel conditioning and moisture on the microleakage of a hydrophilic sealant

Correction

Correction to: Effects of periodontal treatment on carotid intima-media thickness in patients with lifestyle-related diseases: Japanese prospective multicentre observational study

Original Article

Effects of periodontal treatment on carotid intima-media thickness in patients with lifestyle-related diseases: Japanese prospective multicentre observational study

Original Article

Clinical study of guided bone regeneration with resorbable polylactide-co-glycolide acid membrane