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Published in: International Journal of Clinical Oncology 1/2010

01-02-2010 | Original Article

Clinical significance of dihydropyrimidine dehydrogenase and thymidylate synthase expression in patients with pancreatic cancer

Authors: Osamu Nakahara, Hiroshi Takamori, Hiroshi Tanaka, Yasuo Sakamoto, Yoshiaki Ikuta, Satoshi Furuhashi, Masayuki Watanabe, Toru Beppu, Masahiko Hirota, Keiichiro Kanemitsu, Hideo Baba

Published in: International Journal of Clinical Oncology | Issue 1/2010

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Abstract

Background

Little is known about the clinical significance of TS and DPD in pancreatic cancer. We aimed to evaluate TS and DPD expression levels in not only pancreatic cancer but also surrounding normal pancreatic tissues to assess the clinical implications of the expression of TS and DPD in this study.

Patients and methods

Pancreatic cancer and normal pancreatic tissues were obtained from 18 patients with pancreatic cancer who underwent pancreatic resection to measure TS and DPD activities. The TS and DPD activities were determined by enzyme-linked immunosorbent assay using non-fixed fresh-frozen specimens.

Results

Pancreatic cancer tissues had significantly higher DPD and TS enzyme activities than surrounding normal tissue. Anaplastic ductal carcinoma had higher DPD and TS activities than the other histological types. Patients with high DPD in this study demonstrated poorer prognosis than those with low DPD. On the other hand, there was no statistically significant difference in survival between the high and the low TS groups.

Conclusions

The efficacy of 5-FU may be lower in pancreatic cancer tissue than in normal tissue because DPD activity is upregulated in pancreatic cancer tissue compared to normal pancreatic tissue. It is necessary to develop an effective 5-FU delivery system and/or 5-FU combined with an inhibitor for DPD that can be used when 5-FU must be administered to patients with pancreatic cancer. High DPD activity may be a prognostic factor in patients with pancreatic cancer.
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Metadata
Title
Clinical significance of dihydropyrimidine dehydrogenase and thymidylate synthase expression in patients with pancreatic cancer
Authors
Osamu Nakahara
Hiroshi Takamori
Hiroshi Tanaka
Yasuo Sakamoto
Yoshiaki Ikuta
Satoshi Furuhashi
Masayuki Watanabe
Toru Beppu
Masahiko Hirota
Keiichiro Kanemitsu
Hideo Baba
Publication date
01-02-2010
Publisher
Springer Japan
Published in
International Journal of Clinical Oncology / Issue 1/2010
Print ISSN: 1341-9625
Electronic ISSN: 1437-7772
DOI
https://doi.org/10.1007/s10147-009-0008-2

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