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Lasers in Medical Science
Published in: Lasers in Medical Science 8/2021

01-10-2021 | CO2 Laser | Original Article

Bacterial reduction effect of four different dental lasers on titanium surfaces in vitro

Authors: W. Patrick Misischia, Pinelopi Xenoudi, Raymond A. Yukna, Michael J. Schurr

Published in: Lasers in Medical Science | Issue 8/2021

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Abstract

Compare the effectiveness of selected dental lasers for decontamination of machined titanium surfaces in vitro. Seventy-two sterile machined surface titanium discs were individually inoculated with strains of Streptococcus mutans (Sm), Streptococcus oralis (So), Aggregatibacter actinomycetemcomitans (Aa), or all three bacteria together (MIX) at 34.0° C, 20.8% O2 and 5% CO2 for 12 h. After incubation, the discs were divided into six groups: 1) no treatment, 2) 0.12% chlorhexidine gluconate (CHX), and 3) 10,600 CO2, 4) 810 nm diode, 5) 2780 nm Er,Cr:YSGG, 6) 1064 nm Nd:YAG laser groups. After treatment, any remaining viable bacteria were liberated from the discs via sonication, transferred onto brain heart infusion (BHI) agar plates for culturing, and colony-forming units (CFUs) were recorded. Statistical analysis was performed. There were statistically significantly differences (SSD) (p < 0.01) in bacterial reduction of discs individually inoculated with Aa between the Er,Cr:YSGG and Nd:YAG lasers. There was also a SSD (p < 0.01) lower effect with the MIX with the Er,Cr:YSGG compared with all other modalities. Bacterial reduction with the CO2 was better (p < 0.001) than treatment with CHX or the Er,Cr:YSGG laser on killing of So. Although all modalities of treatment showed a mean of 98% or greater viable bacterial reduction, the most consistent bacterial reduction of all titanium discs was with the Nd:YAG laser (100%).
Footnotes
1
All Metal Sales, Inc., 29,260 Clemens Road, Westlake, Ohio 44,145
 
2
10,600 nm CO2 laser, Lutronic Denta 2®, Great Plains Technology, Inc, Fairfield, NE
 
3
810 diode, Picasso®, AMD Lasers, West Jordan, UT
 
4
Er,Cr:YSGG, WaterLase MD®, Biolase, Inc., Irvine, CA
 
5
Nd:YAG, PerioLase MVP-7®, Millennium Dental Technologies, Inc., Cerritos, CA
 
6
PowerMax 600, Coherent, Santa Clara, CA
 
7
Prism 7® version 5.0d, GraphPad Software, Inc., La Jolla, CA
 
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Metadata
Title
Bacterial reduction effect of four different dental lasers on titanium surfaces in vitro
Authors
W. Patrick Misischia
Pinelopi Xenoudi
Raymond A. Yukna
Michael J. Schurr
Publication date
01-10-2021
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 8/2021
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-021-03349-3

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