Skip to main content
Key Specialties
Cardiology Diabetology Endocrinology Gastroenterology Geriatrics and Gerontology Gynecology and Obstetrics Hematology Infectious Disease Internal Medicine Nephrology Neurology Oncology Pediatrics Respiratory Medicine Rheumatology Surgery
Congress Coverage
2024 ACC Congress 2023 ESMO Congress 2023 EASD Congress 2023 ERS Congress 2023 ESC Congress
Clinical Collections
Advances in Alzheimer’s

At a glance: The STEP trials

A round-up of the STEP phase 3 clinical trials evaluating semaglutide for weight loss in people with overweight or obesity.
ADVANCED SEARCH
Log in
Top
Lasers in Medical Science
Published in: Lasers in Medical Science 1/2022

Open Access 01-02-2022 | Laser | Original Article

Photobiomodulation invigorating collagen deposition, proliferating cell nuclear antigen and Ki67 expression during dermal wound repair in mice

Authors: Vijendra Prabhu, Bola Sadashiva Satish Rao, Anuradha Calicut Kini Rao, Keerthana Prasad, Krishna Kishore Mahato

Published in: Lasers in Medical Science | Issue 1/2022

Login to get access

Abstract

The present investigation focuses on understanding the role of photobiomodulation in enhancing tissue proliferation. Circular excision wounds of diameter 1.5 cm were created on Swiss albino mice and treated immediately with 2 J/cm2 and 10 J/cm2 single exposures of the Helium-Neon laser along with sham-irradiated controls. During different days of healing progression (day 5, day 10, and day 15), the tissue samples upon euthanization of the animals were taken for assessing collagen deposition by Picrosirius red staining and cell proliferation (day 10) by proliferating cell nuclear antigen (PCNA) and Ki67. The positive influence of red light on collagen synthesis was found to be statistically significant on day 10 (P < 0.01) and day 15 (P < 0.05) post-wounding when compared to sham irradiation, as evident from the image analysis of collagen birefringence. Furthermore, a significant rise in PCNA (P < 0.01) and Ki67 (P < 0.05) expression was also recorded in animals exposed to 2 J/cm2 when compared to sham irradiation and (P < 0.01) compared to the 10 J/cm2 treated group as evidenced by the microscopy study. The findings of the current investigation have distinctly exhibited the assenting influence of red laser light on excisional wound healing in Swiss albino mice by augmenting cell proliferation and collagen deposition.
Literature
1.
Otterco AN, Andrade AL, Brassolatti P, Pinto KNZ, Araujo HSS, Parizotto NA (2018) Photobiomodulation mechanisms in the kinetics of the wound healing process in rats. J Photochem Photobiol B 183:22–29PubMed
2.
Jonat W, Arnold N (2011) Is the Ki-67 labelling index ready for clinical use? Ann Oncol 22(3):500–502PubMed
3.
Takahashi T, Caviness VS Jr (1993) PCNA-binding to DNA at the G1/S transition in proliferating cells of the developing cerebral wall. J Neurocytol 22(12):1096–1102PubMed
4.
Tsai WC, Cheng JW, Chen JL, Chen CY, Chang HN, Liao YH, Lin MS, Pang JH (2014) Low-level laser irradiation stimulates tenocyte proliferation in association with increased NO synthesis and upregulation of PCNA and cyclins. Lasers Med Sci 29(4):1377–1384PubMed
5.
Heiskanen V, Hamblin MR (2018) Photobiomodulation: lasers vs. light emitting diodes? Photochem Photobiol Sci 17(8):1003–1017PubMedPubMedCentral
6.
Enwemeka CS (2005) Light is light. Photomed Laser Surg 23(2):159–160PubMed
7.
Reddy GK (2003) Comparison of the photostimulatory effects of visible He-Ne and infrared Ga-As lasers on healing impaired diabetic rat wounds. Lasers Surg Med 33(5):344–351PubMed
8.
Jere SW, Abrahamse H, Houreld NN (2017) The JAK/STAT signaling pathway and photobiomodulation in chronic wound healing. Cytokine Growth Factor Rev 38:73–79PubMed
9.
Allendorf JD, Bessler M, Huang J, Kayton ML, Laird D, Nowygrod R, Treat MR (1997) Helium-neon laser irradiation at fluences of 1, 2, and 4 J/cm2 failed to accelerate wound healing as assessed by both wound contracture rate and tensile strength. Lasers Surg Med 20(3):340–345PubMed
10.
Bouma MG, Buurman WA, van den Wildenberg FA (1996) Low energy laser irradiation fails to modulate the inflammatory function of human monocytes and endothelial cells. Lasers Surg Med 19(2):207–215PubMed
11.
Garza ZCF, Born M, Hilbers PAJ, van Riel NAW, Liebmann J (2018) Visible blue light therapy: molecular mechanisms and therapeutic opportunities. Curr Med Chem 25(40):5564–5577PubMed
12.
Prabhu V, Rao SB, Rao NB, Aithal KB, Kumar P, Mahato KK (2010) Development and evaluation of fiber optic probe-based helium-neon low-level laser therapy system for tissue regeneration--an in vivo experimental study. Photochem Photobiol 86(6):1364–1372PubMed
13.
Hegde VN, Prabhu V, Rao SB, Chandra S, Kumar P, Satyamoorthy K, Mahato KK (2011) Effect of laser dose and treatment schedule on excision wound healing in diabetic mice. Photochem Photobiol 87(6):1433–1441PubMed
14.
Prabhu V, Rao SB, Chandra S, Kumar P, Rao L, Guddattu V, Satyamoorthy K, Mahato KK (2012) Spectroscopic and histological evaluation of wound healing progression following low level laser therapy (LLLT). J Biophotonics 5(2):168–184PubMed
15.
Junqueira LC, Cossermelli W, Brentani R (1978) Differential staining of collagens type I, II and III by Sirius red and polarization microscopy. Arch Histol Jpn 41(3):267–274PubMed
16.
Prasad K, Bhagath Kumar P, Chakravarthy M, Prabhu G (2012) Applications of ‘TissueQuant’- a color intensity quantification tool for medical research. Comput Methods Prog Biomed 106(1):27–36
17.
Prabhu V, Rao SB, Fernandes EM, Rao AC, Prasad K, Mahato KK (2014) Objective assessment of endogenous collagen in vivo during tissue repair by laser induced fluorescence. PLoS One 9(5):e98609PubMedPubMedCentral
18.
Mokoena D, Dhilip Kumar SS, Houreld NN, Abrahamse H (2018) Role of photobiomodulation on the activation of the Smad pathway via TGF-beta in wound healing. J Photochem Photobiol B 189:138–144PubMed
19.
Ayuk SM, Abrahamse H, Houreld NN (2018) Photobiomodulation alters matrix protein activity in stressed fibroblast cells in vitro. J Biophotonics 11(3)
20.
Wang Y, Huang YY, Wang Y, Lyu P, Hamblin MR (2017) Red (660 nm) or near-infrared (810 nm) photobiomodulation stimulates, while blue (415 nm), green (540 nm) light inhibits proliferation in human adipose-derived stem cells. Sci Rep 7(1):7781PubMedPubMedCentral
21.
Brown DC, Gatter KC (2002) Ki67 protein: the immaculate deception? Histopathology 40(1):2–11PubMed
22.
Crompton R, Williams H, Ansell D, Campbell L, Holden K, Cruickshank S, Hardman MJ (2016) Oestrogen promotes healing in a bacterial LPS model of delayed cutaneous wound repair. Lab Investig 96(4):439–449PubMed
23.
Gupta A, Dai T, Hamblin MR (2014) Effect of red and near-infrared wavelengths on low-level laser (light) therapy-induced healing of partial-thickness dermal abrasion in mice. Lasers Med Sci 29(1):257–265PubMed
24.
Wu YF, Chen CH, Cao Y, Avanessian B, Wang XT, Tang JB (2010) Molecular events of cellular apoptosis and proliferation in the early tendon healing period. J Hand Surg Am 35(1):2–10PubMed
25.
Khan SZ, Kokubu E, Tsuruoka M, Murakami S, Matsuzaka K, Inoue T (2013) Morphological effect of diode laser irradiation of periapical lesion in rat. Int J Las Dent 3(2):37–43
26.
Hu WP, Wang JJ, Yu CL, Lan CC, Chen GS, Yu HS (2007) Helium-neon laser irradiation stimulates cell proliferation through photostimulatory effects in mitochondria. J Invest Dermatol 127(8):2048–2057PubMed
27.
Li Y, Zhang J, Xu Y, Han Y, Jiang B, Huang L, Zhu H, Xu Y, Yang W, Qin C (2016) The histopathological investigation of red and blue light emitting diode on treating skin wounds in Japanese big-ear white rabbit. PLoS One 11(6):e0157898PubMedPubMedCentral
28.
Noda M, Aoki A, Mizutani K, Lin T, Komaki M, Shibata S, Izumi Y (2016) High-frequency pulsed low-level diode laser therapy accelerates wound healing of tooth extraction socket: an in vivo study. Lasers Surg Med 48(10):955–964PubMed
29.
Shu B, Ni GX, Zhang LY, Li XP, Jiang WL, Zhang LQ (2013) High-power helium-neon laser irradiation inhibits the growth of traumatic scars in vitro and in vivo. Lasers Med Sci 28(3):693–700PubMed
30.
Azevedo LH, de Paula EF, Moreira MS, de Paula EC, Marques MM (2006) Influence of different power densities of LILT on cultured human fibroblast growth : a pilot study. Lasers Med Sci 21(2):86–89PubMed
31.
Cuttle L, Nataatmadja M, Fraser JF, Kempf M, Kimble RM, Hayes MT (2005) Collagen in the scarless fetal skin wound: detection with picrosirius-polarization. Wound Repair Regen 13(2):198–204PubMed
32.
Walker MD, Rumpf S, Baxter GD, Hirst DG, Lowe AS (2000) Effect of low-intensity laser irradiation (660 nm) on a radiation-impaired wound-healing model in murine skin. Lasers Surg Med 26(1):41–47PubMed
33.
Rittie L (2017) Method for Picrosirius red-polarization detection of collagen fibers in tissue sections. Methods Mol Biol 1627:395–407PubMed
34.
Schulz R, Gres P, Konietzka I, Heusch G (2005) Regional differences of myocardial infarct development and ischemic preconditioning. Basic Res Cardiol 100(1):48–56PubMed
35.
Ophof R, Maltha JC, Von den Hoff JW, Kuijpers-Jagtman AM (2004) Histologic evaluation of skin-derived and collagen-based substrates implanted in palatal wounds. Wound Repair Regen 12(5):528–538PubMed
36.
Baptista J, Martins MD, Pavesi VC, Bussadori SK, Fernandes KP, Pinto Junior Ddos S, Ferrari RA (2011) Influence of laser photobiomodulation on collagen IV during skeletal muscle tissue remodeling after injury in rats. Photomed Laser Surg 29(1):11–17PubMed
37.
de Souza TO, Mesquita DA, Ferrari RA, Dos Santos Pinto D Jr, Correa L, Bussadori SK, Fernandes KP, Martins MD (2011) Phototherapy with low-level laser affects the remodeling of types I and III collagen in skeletal muscle repair. Lasers Med Sci 26(6):803–814PubMed
38.
de Sousa AP, Santos JN, Dos Reis JA Jr, Ramos TA, de Souza J, Cangussu MC, Pinheiro AL (2010) Effect of LED phototherapy of three distinct wavelengths on fibroblasts on wound healing: a histological study in a rodent model. Photomed Laser Surg 28(4):547–552PubMed
39.
Kerppers II, de Lima CJ, Fernandes AB, Villaverde AB (2015) Effect of light-emitting diode ( 627 nm and 945 nm ) treatment on first intention healing: immunohistochemical analysis. Lasers Med Sci 30(1):397–401PubMed
40.
Pugliese LS, Medrado AP, Reis SR, Andrade Zde A (2003) The influence of low-level laser therapy on biomodulation of collagen and elastic fibers. Pesqui Odontol Bras 17(4):307–313PubMed
41.
Cunha JLS, Carvalho FMA, Pereira Filho RN, Ribeiro MAG, de Albuquerque-Junior RLC (2019) Effects of different protocols of low-level laser therapy on collagen deposition in wound healing. Braz Dent J 30(4):317–324PubMed
42.
Aparecida Da Silva A, Leal-Junior EC, Alves AC, Rambo CS, Dos Santos SA, Vieira RP, De Carvalho PT (2013) Wound-healing effects of low-level laser therapy in diabetic rats involve the modulation of MMP-2 and MMP-9 and the redistribution of collagen types I and III. J Cosmet Laser Ther 15(4):210–216PubMed
43.
Demir H, Yaray S, Kirnap M, Yaray K (2004) Comparison of the effects of laser and ultrasound treatments on experimental wound healing in rats. J Rehabil Res Dev 41(5):721–728PubMed
44.
Oliveira Sampaio SC, de C Monteiro JS, Cangussu MC, Pires Santos GM, dos Santos MA, dos Santos JN, Pinheiro AL (2013) Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers Med Sci 28(3):799–806PubMed
45.
Silveira PC, Silva LA, Freitas TP, Latini A, Pinho RA (2011) Effects of low-power laser irradiation (LPLI) at different wavelengths and doses on oxidative stress and fibrogenesis parameters in an animal model of wound healing. Lasers Med Sci 26(1):125–131PubMed
Metadata
Title
Photobiomodulation invigorating collagen deposition, proliferating cell nuclear antigen and Ki67 expression during dermal wound repair in mice
Authors
Vijendra Prabhu
Bola Sadashiva Satish Rao
Anuradha Calicut Kini Rao
Keerthana Prasad
Krishna Kishore Mahato
Publication date
01-02-2022
Publisher
Springer London
Keyword
Laser
Published in
Lasers in Medical Science / Issue 1/2022
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-020-03202-z

Other articles of this Issue 1/2022

Lasers in Medical Science 1/2022 Go to the issue

Original Article

Near-infrared 940-nm diode laser photobiomodulation of inflamed periodontal ligament stem cells

Original Article

Photobiomodulation therapy was more effective than photobiomodulation plus arginine on accelerating wound healing in an animal model of delayed healing wound

Original Article

The effectiveness of high-intensity laser therapy in the treatment of post-stroke patients with hemiplegic shoulder pain: a prospective randomized controlled study

Original Article

Effects of laser acupuncture tele-therapy for rheumatoid arthritis elderly patients

Original Article

Diode laser versus bipolar diathermy for turbinate reduction in cases of inferior turbinate hypertrophy

Original Article

Evaluation of a novel deep tissue transvaginal near-infrared laser and applicator in an ovine model