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01-07-2021 | Thalassemia | Original Article

Using LIBS as a diagnostic tool in pediatrics beta-thalassemia

Authors: Jehan Alsharnoubi, Yasser Nassef, Reham F. Fahmy, Mohamed Gamal

Published in: Lasers in Medical Science | Issue 5/2021

Abstract

Beta-thalassemia major is a common inherited single-gene disorder. Thalassemic patients are at risk of changes in some important trace elements. To detect alteration of iron, copper, zinc, and calcium serum levels in beta-thalassemia major patients, laser-induced breakdown spectroscopy (LIBS) was used. This study was conducted on 40 beta-thalassemia major and 40 healthy young patients (age: 12–18 years old; male:female = 1:1). Venous blood samples were collected from both groups and analyzed for the serum levels of iron, calcium, zinc, and copper by exposing the samples to LIBS. The intensities of the tested elements were detected using the Kestrel Spec computer software and analyzed with an SPSS 25 program. Thalassemic patients had significantly higher serum iron (p = < 0.001) and copper (p = < 0.005) while they had significantly lower serum zinc (p = < 0.005) and calcium (p= > 0.005) when compared with control. Also, thalassemic patients had significantly lower body weight and height as they were less than the 3rd percentile by 82.5% and p < 0.001. LIBS is a safe and efficient tool to detect alteration of some serum trace elements in beta-thalassemia patients.

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Title: Using LIBS as a diagnostic tool in pediatrics beta-thalassemia
Authors: Jehan Alsharnoubi
Yasser Nassef
Reham F. Fahmy
Mohamed Gamal
Publication date: 01-07-2021
Publisher: Springer London
Keywords: Thalassemia
Laser
Published in: Lasers in Medical Science / Issue 5/2021
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-020-03117-9

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