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Lasers in Medical Science
Published in: Lasers in Medical Science 9/2020

01-12-2020 | Original Article

Irradiation with blue light-emitting diode enhances osteogenic differentiation of stem cells from the apical papilla

Authors: Yaoyao Yang, Tingting Zhu, Yan Wu, Chunxia Shu, Qiang Chen, Juan Yang, Xiang Luo, Yao Wang

Published in: Lasers in Medical Science | Issue 9/2020

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Abstract

This study aimed to evaluate the effects of low-energy blue LED irradiation on the osteogenic differentiation of stem cells from the apical papilla (SCAPs). SCAPs were derived from human tooth root tips and were irradiated with 0 (control group), 1 J/cm2, 2 J/cm2, 3 J/cm2, or 4 J/cm2 blue light in osteogenic induction medium. Cell proliferation was analyzed using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyl tetrazolium bromide (MTT) assay. Osteogenic differentiation activity was evaluated by monitoring alkaline phosphatase (ALP), alizarin red staining, and real-time polymerase chain reaction (RT-PCR). The results of the MTT assay indicated that SCAPs in the LED groups exhibited a lower proliferation rate than those in the control group, and there were statistically differences between the 2 J/cm2, 3 J/cm2, and 4 J/cm2 groups and the control group (P < 0.05). The results of the ALP and alizarin red analyses showed that blue LED promoted osteogenic differentiation of the SCAPs. And 4 J/cm2 blue light upregulates the expression levels of the osteogenic/dentinogenic genes ALP, dentin sialophosphoprotein (DSPP), dentin matrix protein-1 (DMP-1), and osteocalcin (OCN) in SCAPs. Our results confirmed that low-energy blue LED at 1 J/cm2, 2 J/cm2, 3 J/cm2, and 4 J/cm2 could inhibit the proliferation of SCAPs and promotes osteogenic differentiation of SCAPs. Further in vitro studies are required to explore the mechanisms of the effects by low-energy blue LED.
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Metadata
Title
Irradiation with blue light-emitting diode enhances osteogenic differentiation of stem cells from the apical papilla
Authors
Yaoyao Yang
Tingting Zhu
Yan Wu
Chunxia Shu
Qiang Chen
Juan Yang
Xiang Luo
Yao Wang
Publication date
01-12-2020
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 9/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-020-02995-3

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