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Published in:

01-03-2020 | Review Article

Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging

Authors: Pranoy Sahu, Nirmal Mazumder

Published in: Lasers in Medical Science | Issue 2/2020

Abstract

Deep tissue imaging using two-photon fluorescence (TPF) techniques have revolutionized the optical imaging community by providing in depth molecular information at the single-cell level. These techniques provide structural and functional aspects of mammalian brain at unprecedented depth and resolution. However, wavefront distortions introduced by the optical system as well as the biological sample (tissue) limit the achievable fluorescence signal-to-noise ratio and resolution with penetration depth. In this review, we discuss on the advances in TPF microscopy techniques for in vivo functional imaging and offer guidelines as to which technologies are best suited for different imaging applications with special reference to adaptive optics.

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Title: Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging
Authors: Pranoy Sahu
Nirmal Mazumder
Publication date: 01-03-2020
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 2/2020
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-019-02908-z

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Springer Medicine

Advances in adaptive optics–based two-photon fluorescence microscopy for brain imaging

Abstract

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Abstract

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