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01-04-2019 | Original Article

Physicochemical, morphological, and biological analyses of Ti-15Mo alloy surface modified by laser beam irradiation

Authors: Luana C. Pires, Fernando P. S. Guastaldi, Andressa V. B. Nogueira, Nilson T. C. Oliveira, Antonio C. Guastaldi, Joni A. Cirelli

Published in: Lasers in Medical Science | Issue 3/2019

Abstract

Perform a physicochemical and morphological characterization of a Ti-15Mo alloy surface modified by laser beam irradiation and to evaluate in vitro the morphological response and proliferation of osteoblastic cells seeded onto this alloy. Disks were made of two different metals, Ti-15Mo alloy and cpTi, used as control. A total of four groups were evaluated: polished cpTi (cpTi-pol), laser-irradiated cpTi (cpTi-L), polished Ti-15Mo alloy (Ti-15Mo-pol), and laser-irradiated Ti-15Mo alloy (Ti-15Mo-L). Before and after laser irradiation of the surfaces, physicochemical and morphological analyses were performed: scanning electron microscopy (FEG-SEM), energy-dispersive spectroscopy (EDX), and X-ray diffraction (XRD). The wettability of the samples was evaluated by contact angle measurement. Murine preosteoblastic cells MC3T3-E1 were cultured onto the experimental disks for cell proliferation, morphology, and spreading analyses. Laser groups presented irregular-shaped cavities on its surface and a typical microstructured surface with large depressions (FEG-SEM). The contact angle for both laser groups was 0°, whereas for the polished groups was ≈ 77 and ≈ 78 for cpTi-pol and Ti-15Mo-pol, respectively. Cell proliferation analysis demonstrated a higher metabolic activity in the laser groups (p < 0.05). From the fluorescence microscopy, Ti-15Mo-L surface seems to induce greater cellular differentiation compared to the cpTi-L surface. The preliminary biological in vitro analyses suggested possible advantages of laser surface treatment in the Ti-15Mo alloy regarding cell proliferation and maturation.

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Title: Physicochemical, morphological, and biological analyses of Ti-15Mo alloy surface modified by laser beam irradiation
Authors: Luana C. Pires
Fernando P. S. Guastaldi
Andressa V. B. Nogueira
Nilson T. C. Oliveira
Antonio C. Guastaldi
Joni A. Cirelli
Publication date: 01-04-2019
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 3/2019
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-018-2626-2

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Physicochemical, morphological, and biological analyses of Ti-15Mo alloy surface modified by laser beam irradiation

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