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01-08-2018 | Original Article

Comparative effect of photobiomodulation associated with dexamethasone after sciatic nerve injury model

Authors: Luana Gabriel de Souza, Alexandre Márcio Marcolino, Heloyse Uliam Kuriki, Elaine Cristina Dalazen Gonçalves, Marisa de Cássia Registro Fonseca, Rafael Inácio Barbosa

Published in: Lasers in Medical Science | Issue 6/2018

Abstract

To analyze the effect of photobiomodulation and dexamethasone on nerve regeneration after a sciatic nerve crushing model. Twenty-six Swiss mice were divided into the following groups: naive; sham; injured, low-level laser therapy (LLLT) (660 nm, 10 J/cm², 0.6 J, 16.8 J total energy emitted during the 28 days of radiation, 20 s, for 28 days); dexamethasone (Dex) (local injection of 2 mg/kg for 10 consecutive days); and LLLT group associated with Dex (LLLT/Dex), with the same parameters of the other groups. For nerve injury, a portable adjustable pinch was used. The animals were evaluated using the Sciatic Functional Index (SFI) and Sciatic Static Index (SSI). The results obtained were evaluated with Image J™ and Kinovea™. Data and images were obtained at baseline and after 7, 14, 21, and 28 days after surgery. The evaluation of hyperalgesia, using Hargreaves, and behavior through the open field was also performed. In functional and static analysis, all groups presented significant differences when compared to the injured group. In the analysis of the SSI results, the group treated with both LLLT and dexamethasone was more effective in improving the values of this parameter, and in the SFI, the laser-treated group obtained better results. In the evaluation through the open field and the Hargreaves, there was no difference. The application of LLLT and dexamethasone was effective in nerve regeneration according to the results and was more effective when LLLT was associated with dexamethasone than in LLLT alone for the SSI.

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Title: Comparative effect of photobiomodulation associated with dexamethasone after sciatic nerve injury model
Authors: Luana Gabriel de Souza
Alexandre Márcio Marcolino
Heloyse Uliam Kuriki
Elaine Cristina Dalazen Gonçalves
Marisa de Cássia Registro Fonseca
Rafael Inácio Barbosa
Publication date: 01-08-2018
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 6/2018
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-018-2494-9

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Comparative effect of photobiomodulation associated with dexamethasone after sciatic nerve injury model

Abstract

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Abstract

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