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01-05-2018 | Original Article

High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats

Authors: Caroline Pereira Santos, Andreo Fernando Aguiar, Ines Cristina Giometti, Thaoan Bruno Mariano, Carlos Eduardo Assumpção de Freitas, Gisele Alborghetti Nai, Selma Zambelli de Freitas, Maeli Dal Pai-Silva, Francis Lopes Pacagnelli

Published in: Lasers in Medical Science | Issue 4/2018

Abstract

The aim of this study was to determine the effects of gallium arsenide (GaAs) laser on IGF-I, MyoD, MAFbx, and TNF-α gene expression during the intermediate phase of muscle regeneration after cryoinjury 21 Wistar rats were divided into three groups (n = 7 per group): untreated with no injury (control group), cryoinjury without GaAs (injured group), and cryoinjury with GaAs (GaAs-injured group). The cryoinjury was induced in the central region of the tibialis anterior muscle (TA). The region injured was irradiated once a day during 14 days using GaAs laser (904 nm; spot size 0.035 cm², output power 50 mW; energy density 69 J cm⁻²; exposure time 4 s per point; final energy 4.8 J). Twenty-four hours after the last application, the right and left TA muscles were collected for histological (collagen content) and molecular (gene expression of IGF-I, MyoD, MAFbx, and TNF-α) analyses, respectively. Data were analyzed using one-way ANOVA at P < 0.05. There were no significant (P > 0.05) differences in collagen density and IGF-I gene expression in all experimental groups. There were similar (P < 0.05) decreases in MAFbx and TNF-α gene expression in the injured and GaAs-injured groups, compared to control group. The MyoD gene expression increased (P = 0.008) in the GaAs-injured group, but not in the injured group (P = 0.338), compared to control group. GaAs laser therapy had a positive effect on MyoD gene expression, but not IGF-I, MAFbx, and TNF-α, during intermediary phases (14 days post-injury) of muscle repair.

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Title: High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats
Authors: Caroline Pereira Santos
Andreo Fernando Aguiar
Ines Cristina Giometti
Thaoan Bruno Mariano
Carlos Eduardo Assumpção de Freitas
Gisele Alborghetti Nai
Selma Zambelli de Freitas
Maeli Dal Pai-Silva
Francis Lopes Pacagnelli
Publication date: 01-05-2018
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 4/2018
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-018-2439-3

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

High final energy of gallium arsenide laser increases MyoD gene expression during the intermediate phase of muscle regeneration after cryoinjury in rats

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

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