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Published in: Lasers in Medical Science 1/2018

01-01-2018 | Original Article

Enhanced survival of ischemic skin flap by combined treatment with bone marrow-derived stem cells and low-level light irradiation

Authors: Jeong Hwan Moon, Yun-Hee Rhee, Jin-Chul Ahn, Bongkyun Kim, Sang Joon Lee, Phil-Sang Chung

Published in: Lasers in Medical Science | Issue 1/2018

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Abstract

The aim of this study is to examine the enhanced survival effect of ischemic skin flap by combined treatment with bone marrow-derived stem cells (BMSCs) and low-level light irradiation (LLLI). The neovasculogenic effect of BMSCs induced by LLLI was detected using a wound healing and tube formation assay. ICR mice were divided into four groups: control group, LLLI group, BMSCs group, and combine-treated group. The percentage of skin flap necrosis area was calculated on the seventh post-operative day. Specimens were harvested for histologic analyses. LLLI promoted BMSC migration and tube formation. The flap survival rate of combined treated group was significantly higher than that of the control group. Histologic results demonstrated a significant increase in neovascularization in the combined treatment group. This study demonstrates that combination treatment of BMSCs and LLLI could enhance the survival of ischemic skin flap in a mouse model.
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Metadata
Title
Enhanced survival of ischemic skin flap by combined treatment with bone marrow-derived stem cells and low-level light irradiation
Authors
Jeong Hwan Moon
Yun-Hee Rhee
Jin-Chul Ahn
Bongkyun Kim
Sang Joon Lee
Phil-Sang Chung
Publication date
01-01-2018
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 1/2018
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-017-2312-9

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