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Lasers in Medical Science
Published in: Lasers in Medical Science 8/2017

01-11-2017 | Original Article

Photobiomodulation therapy improves both inflammatory and fibrotic parameters in experimental model of lung fibrosis in mice

Authors: Robson Alexandre Brochetti, Mayara Peres Leal, Raíssa Rodrigues, Renata Kelly da Palma, Luis Vicente Franco de Oliveira, Anna Carolina Ratto Tempestini Horliana, Amílcar Sabino Damazo, Ana Paula Ligeiro de Oliveira, Rodolfo Paula Vieira, Adriana Lino-dos-Santos-Franco

Published in: Lasers in Medical Science | Issue 8/2017

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Abstract

Lung fibrosis (LF) is a chronic and progressive lung disease characterized by pulmonary parenchyma progressive lesion, inflammatory infiltration, and interstitial fibrosis. It is developed by excessive collagen deposition and other cellular matrix components, resulting in severe changes in the alveolar architecture. Considering the absence of effective treatment, the aim of this study was to investigate the effect of photobiomodulation therapy (PBMT) on the development of PF. For this purpose, we used C57BL6 mice subjected to induction of LF by bleomycin administration (1.5 U/kg) by orotracheal route and, after 14 days of the induction, mice were treated with PBMT applied to the thorax 1×/day for 8 days (wavelength 660 ± 20 nm, power 100 mW, radiant exposure 5 J/cm2, irradiance 33.3 mW/cm2, spot size 2.8cm2, total energy 15 J, time of irradiation: 150 s) and inflammatory and fibrotic parameters were evaluated with or without PBMT. Our results showed that PBMT significantly reduced the number of inflammatory cells in the alveolar space, collagen production, interstitial thickening, and static and dynamic pulmonary elastance. In addition, we observed reduced levels of IL-6 e CXCL1/KC released by pneumocytes in culture as well as reduced level of CXCL1/KC released by fibroblasts in culture. We can conclude that the PBMT improves both inflammatory and fibrotic parameters showing a promising therapy which is economical and has no side effects.
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Metadata
Title
Photobiomodulation therapy improves both inflammatory and fibrotic parameters in experimental model of lung fibrosis in mice
Authors
Robson Alexandre Brochetti
Mayara Peres Leal
Raíssa Rodrigues
Renata Kelly da Palma
Luis Vicente Franco de Oliveira
Anna Carolina Ratto Tempestini Horliana
Amílcar Sabino Damazo
Ana Paula Ligeiro de Oliveira
Rodolfo Paula Vieira
Adriana Lino-dos-Santos-Franco
Publication date
01-11-2017
Publisher
Springer London
Published in
Lasers in Medical Science / Issue 8/2017
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-017-2281-z

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