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Lasers in Medical Science
Published in: Lasers in Medical Science 8/2017

01-11-2017 | Original Article

Curcumin photodynamic effect in the treatment of the induced periodontitis in rats

Published in: Lasers in Medical Science | Issue 8/2017

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Abstract

This study assessed the effect of curcumin as a photosensitizer in antimicrobial photodynamic therapy (aPDT) for the treatment of induced periodontitis in rats. Periodontitis was induced via a ligature around the mandibular first molar on the left side of 96 rats. The ligature was removed 7 days later, and the animals were randomized into four groups: NT, no local treatment; CUR, irrigation with curcumin solution (40 μM); LED, irradiation with a light-emitting diode (LED, InGaN, 465–485 nm, 200 mW/cm2, 60 s); and aPDT, irrigation with curcumin solution (40 μM) followed by irradiation with LED. Eight animals from each group were euthanized at 7, 15, and 30 days post-treatment. Treatments were assessed using alveolar bone loss (ABL) in the furcation region using histological, histometric, and immunohistochemical analyses. Rats treated with aPDT exhibited less ABL at 7 days compared to the NT group, moderate pattern immunolabeling for osteoprotegerin at 30 days, and a pattern of immunolabeling for RANKL from moderate to low. Treatments resulted in smaller numbers of TRAP-positive cells compared to the NT group. aPDT as monotherapy using curcumin as a photosensitizer and LED as the light source was effective in the treatment of induced periodontitis in rats.
Literature
1.
2.
Caton JG C, Ciancio SG, Blieden TM, Bradshaw M, Crout RJ, Hefti AF, Massaro JM, Polson AM, Thomas J, Walker C (2001) Subantimicrobial dose doxycycline as an adjunct to scaling and root planing: post-treatment effects. J Clin Periodontol 28:782–789CrossRefPubMed
3.
Maisch T (2007) Anti-microbial photodynamic therapy: useful in the future? Lasers Med Sci 22:83–91CrossRefPubMed
4.
Kömerik N, Nakanishi H, MacRobert AJ, Henderson B, Speight P, Wilson M (2003) In vivo killing of Porphyromonas gingivalis by toluidine blue-mediated photosensitization in an animal model. Antimicrob Agents Chemother 47:932–940CrossRefPubMedPubMedCentral
5.
Qin YL, Luan XL, Bi LJ, Sheng YQ, Zhou CN, Zhang ZG (2008) Comparison of toluidine blue-mediated photodynamic therapy and conventional scaling treatment for periodontitis in rats. J Periodontal Res 43:162–167CrossRefPubMed
6.
de Almeida JM, Theodoro LH, Bosco AF, Nagata MJH, Oshiiwa M, Garcia VG (2007) Influence of photodynamic therapy on the development of ligature-induced periodontitis in rats. J Periodontol 78:566–575CrossRefPubMed
7.
de Almeida JM, Theodoro LH, Bosco AF, Nagata MJH, Bonfante S, Garcia VG (2008) Treatment of experimental periodontal disease by photodynamic therapy in rats with diabetes. J Periodontol 79:2156–2165CrossRefPubMed
8.
de Almeida JM, Theodoro LH, Bosco AF, Nagata MJH, Oshiiwa M, Garcia VG (2008) In vivo effect of photodynamic therapy on periodontal bone loss in dental furcations. J Periodontol 79:1081–1088CrossRefPubMed
9.
Garcia VG, Longo M, Fernandes LA, Junior ECG, Dos Santos SC, Bosco AF, Nagata MJ, Theodoro LH (2013) Treatment of experimental periodontitis in rats using repeated adjunctive antimicrobial photodynamic therapy. Lasers Med Sci 28:143–150CrossRefPubMed
10.
Garcia VG, Longo M, Gualberto Júnior EC, Bosco AF, Nagata MJH, Ervolino E, Theodoro LH (2014) Effect of the concentration of phenothiazine photosensitizers in antimicrobial photodynamic therapy on bone loss and the immune inflammatory response of induced periodontitis in rats. J Periodontal Res 49:584–594CrossRefPubMed
11.
Braun A, Dehn C, Krause F, Jepsen S (2008) Short-term clinical effects of adjunctive antimicrobial photodynamic therapy in periodontal treatment: a randomized clinical trial. J Clin Periodontol 35:877–884CrossRefPubMed
12.
Polansky R, Haas M, Heschl A, Wimmer G (2009) Clinical effectiveness of photodynamic therapy in the treatment of periodontitis. J Clin Periodontol 36:575–580CrossRefPubMed
13.
Chondros P, Nikolidakis D, Christodoulides N, Rössler R, Gutknecht N, Sculean A (2009) Photodynamic therapy as adjunct to non-surgical periodontal treatment in patients on periodontal maintenance: a randomized controlled clinical trial. Lasers Med Sci 24:681–688CrossRefPubMed
14.
Pinheiro SL, Donegá JM, Seabra LM, Adabo MD, Lopes T, do Carmo TH, Ribeiro MC, Bertolini PF (2010) Capacity of photodynamic therapy for microbial reduction in periodontal pockets. Lasers Med Sci 25:87–91CrossRefPubMed
15.
Theodoro LH, Silva SP, Pires JR, Soares GH, Pontes AE, Zuza EP, Spolidório DM, de Toledo BE, Garcia VG (2012) Clinical and microbiological effects of photodynamic therapy associated with nonsurgical periodontal treatment. A 6-month follow-up. Lasers Med Sci 27:687–693CrossRefPubMed
16.
Alwaeli HA, Al-khateeb SN, Al-sadi A (2015) Long-term clinical effect of adjunctive antimicrobial photodynamic therapy in periodontal treatment: a randomized clinical trial. 30:801–807
17.
Betsy J, Prasanth CS, Baiju KV, Prasanthila J, Subhash N (2014) Efficacy of antimicrobial photodynamic therapy in the management of chronic periodontitis: a randomized controlled clinical trial. J Clin Periodontol 41:573–581CrossRefPubMed
18.
Monzavi A, Chinipardaz Z, Mousavi M, Fekrazad R, Moslemi N, Azaripour A, Bagherpasand O, Chiniforush N (2016) Antimicrobial photodynamic therapy using diode laser activated indocyanine green as an adjunct in the treatment of chronic periodontitis: a randomized clinical trial. Photodiagn Photodyn Ther 14:93–97CrossRef
19.
Wainwright M (2008) Photodynamic therapy: the development of new photosensitisers. Anti Cancer Agents Med Chem 8:280–291CrossRef
20.
Haukvik T, Bruzell E, Kristensen S, Tonnesen HH (2009) Photokilling of bacteria by curcumin in different aqueous preparations. Studies on curcumin and curcuminoids XXXVII Pharmazie 64:666–673PubMed
21.
Bruzell EM, Morisbak E, Tonnesen HH (2005) Studies on curcumin and curcuminoids. XXIX. Photoinduced cytotoxicity of curcumin in selected aqueous preparations. Photochem Photobiol Sci 4:523–530CrossRefPubMed
22.
Wikene KO, Hegge AB, Ellen Bruzell HHT (2015) Formulation and characterization of lyophilized curcumin solid dispersions for antimicrobial photodynamic therapy (aPDT): studies on curcumin and curcuminoids LII. Drug Dev Ind Pharm 41:969–977CrossRefPubMed
23.
Aggarwal BB, Deb L, Prasad S (2015) Curcumin differs from tetrahydrocurcumin for molecular targets, signaling pathways and cellular responses. Molecules 20:185–205CrossRef
24.
Martins CVB, da Silva DL, Neres AT, Magalhães TF, Watanabe GA, Modolo LV, Sabino AA, de Fátima A, de Resende MA (2009) Curcumin as a promising antifungal of clinical interest. J Antimicrob Chemother 63:337–339CrossRefPubMed
25.
Dujic J, Kippenberger S, Ramirez-Bosca A, Diaz-Alperi J, Bereiter-Hahn J, Kaufmann R, Bernd A, Hofmann M (2009) Curcumin in combination with visible light inhibits tumor growth in a xenograft tumor model. Int J Cancer 124:1422–1428CrossRefPubMed
26.
Araújo NC, Fontana CR, Gerbi MEM, Bagnato VS (2012) Overall-mouth disinfection by photodynamic therapy using curcumin. Photomed Laser Surg 30:96–101CrossRefPubMed
27.
Dovigo LN, Pavarina AC, Carmello JC, MacHado AL, Brunetti IL, Bagnato VS (2011) Susceptibility of clinical isolates of Candida to photodynamic effects of curcumin. Lasers Surg Med 43:927–934CrossRefPubMed
28.
Diego, Leite V, Paolillo FR, Parmesano TN, Fontana CR, Bagnato VS (2014) Effects of photodynamic therapy with blue light and curcumin as mouth rinse for oral disinfection. Photomed Laser Surg 32:627–632CrossRef
29.
Sreedhar A, Sarkar I, Rajan P, Pai J, Malagi S, Kamath V, Barmappa R (2015) Comparative evaluation of the efficacy of curcumin gel with and without photo activation as an adjunct to scaling and root planing in the treatment of chronic periodontitis: a split mouth clinical and microbiological study. J Nat Sci Biol Med 6:102–109CrossRef
30.
Paschoal MA, Moura CM, Jeremias F, Souza JF, Bagnato VS, Giusti JS, Santos-Pinto L (2015) Longitudinal effect of curcumin-photodynamic antimicrobial chemotherapy in adolescents during fixed orthodontic treatment: a single-blind randomized clinical trial study. Lasers Med Sci 30:2059–2065CrossRefPubMed
31.
Gualberto  EC Jr, Theodoro LH, Longo M, Novaes VCN, Nagata MJH, Ervolino E, Garcia VG (2016) Antimicrobial photodynamic therapy minimizes the deleterious effect of nicotine in female rats with induced periodontitis. Lasers Med Sci 31:83–94
32.
Garcia VG, Fernandes LA, Macarini VC, de Almeida JM, Martins TM, Bosco AF, Nagata MJ, Cirelli JA, Theodoro LH (2011) Treatment of experimental periodontal disease with antimicrobial photodynamic therapy in nicotine-modified rats. J Clin Periodontol 38:1106–1114CrossRefPubMed
33.
Guimarães MR, Coimbra LS, De Aquino SG, Spolidorio LC, Kirkwood KL, Rossa C (2011) Potent anti-inflammatory effects of systemically administered curcumin modulate periodontal disease in vivo. J Periodontal Res 46:269–279CrossRefPubMedPubMedCentral
34.
Tønnesen HH, de Vries H, Karlsen J, Beijersbergen van Henegouwen G (1987) Studies on curcumin and curcuminoids. IX: Investigation of the photobiological activity of curcumin using bacterial indicator systems. J Pharm Sci 76:371–373CrossRefPubMed
35.
Chang PC, Chien LY, Ye Y, Kao MJ (2013) Irradiation by light-emitting diode light as an adjunct to facilitate healing of experimental periodontitis in vivo. J Periodontal Res 48:135–143CrossRefPubMed
36.
Oliveira Sampaio SCP, de C Monteiro JS, Cangussú MC, Pires Santos GM, dos Santos MA, dos Santos JN, Pinheiro AL (2013) Effect of laser and LED phototherapies on the healing of cutaneous wound on healthy and iron-deficient Wistar rats and their impact on fibroblastic activity during wound healing. Lasers Med Sci 28:799–806CrossRefPubMed
37.
Carvalho AS, Napimoga MH, Coelho-Campos J, Silva-Filho VJ, Thedei G (2011) Photodynamic therapy reduces bone resorption and decreases inflammatory response in an experimental rat periodontal disease model. Photomed Laser Surg 29:735–740CrossRefPubMed
38.
Ozawa Y, Shimizu N, Kariya G, Abiko Y (1998) Low-energy laser irradiation stimulates bone nodule formation at early stages of cell culture in rat calvarial cells. Bone 22:347–354CrossRefPubMed
39.
Zhou T, Chen D, Li Q, Sun X, Song Y, Wang C (2013) Curcumin inhibits inflammatory response and bone loss during experimental periodontitis in rats. Acta Odontol Scand 71:349–356CrossRefPubMed
40.
Bhatia M, Urolagin SS, Pentyala KB, Urolagin SB, Menaka KB, Bhoi S (2014) Novel therapeutic approach for the treatment of periodontitis by curcumin. J Clin Diagnostic Res 8:65–69
41.
Pileggi G, Wataha JC, Girard M, Grad I, Schrenzel J, Lange N, Bouillaguet S (2013) Blue light-mediated inactivation of enterococcus faecalis in vitro. Photodiagn Photodyn Ther 10:134–140CrossRef
42.
Araújo NC, Fontana CR, Bagnato VS, Gerbi MEM (2012) Photodynamic effects of curcumin against cariogenic pathogens. Photomed Laser Surg 30:393–399CrossRefPubMed
43.
Theodoro LH, Longo M, Ervolino E, Duque C, Ferro-Alves ML, Assem NZ, Louzada LM, Garcia VG (2016) Effect of low-level laser therapy as an adjuvant in the treatment of periodontitis induced in rats subjected to 5-fluorouracil chemotherapy. J Periodontal Res 51:669–680CrossRefPubMed
44.
Graves DT, Fine D, Teng YT, Van Dyke TE, Hajishengallis G (2008) The use of rodent models to investigate host-bacteria interactions related to periodontal diseases. J Clin Periodontol 35:89–105CrossRefPubMedPubMedCentral
Metadata
Title
Curcumin photodynamic effect in the treatment of the induced periodontitis in rats
Publication date
01-11-2017
Published in
Lasers in Medical Science / Issue 8/2017
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI
https://doi.org/10.1007/s10103-017-2261-3

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