Top

Published in:

01-11-2016 | Original Article

Laser biostimulation of wound healing: bioimpedance measurements support histology

Authors: Hakan Solmaz, Sergulen Dervisoglu, Murat Gulsoy, Yekta Ulgen

Published in: Lasers in Medical Science | Issue 8/2016

Abstract

Laser biostimulation in medicine has become widespread supporting the idea of therapeutic effects of photobiomodulation in biological tissues. The aim of this study was to investigate the biostimulation effect of laser irradiation on healing of cutaneous skin wounds, in vivo, by means of bioimpedance measurements and histological examinations. Cutaneous skin wounds on rats were subjected to 635 nm diode laser irradiations at two energy densities of 1 and 3 J/cm² separately. Changes in the electrical properties of the wound sites were examined with multi-frequency electrical impedance measurements performed on the 3rd, 7th, 10th, and 14th days following the wounding. Tissue samples were both morphologically and histologically examined to determine the relationship between electrical properties and structure of tissues during healing. Laser irradiations of both energy densities stimulated the wound healing process. In particular, laser irradiation of lower energy density had more evidence especially for the first days of healing process. On the 7th day of healing, 3 J/cm² laser-irradiated tissues had significantly smaller wound areas compared to non-irradiated wounds (p < 0.05). The electrical impedance results supported the idea of laser biostimulation on healing of cutaneous skin wounds. Thus, bioimpedance measurements may be considered as a non-invasive supplementary method for following the healing process of laser-irradiated tissues.

Gulsoy M, Dereli Z, Tabakoglu HO, Bozkulak O (2006) Closure of skin incisions by 980-nm diode laser welding. Lasers Med Sci 21:5–10CrossRefPubMed

Diegelmann RF, Evans MC (2004) Wound healing: an overview of acute, fibrotic and delay healing. Front Biosci 9:283–289CrossRefPubMed

Monaco JL, Lawrence T (2003) Acute wound healing: an overview. Clinics in Plastic Surg 30:1–12CrossRef

Karu T (1999) Primary and secondary mechanisms of action of visible to near-IR radiation on cells. J Photochem Photobiol B 49:1–17CrossRefPubMed

Karu T (1989) Photobiology of low-power laser effects. Health Phys 56:691–704CrossRefPubMed

Karu T (1990) Effects of visible radiation on cultured cells. Photochem Photobiol 52:1089–1098CrossRefPubMed

AlGhamdi KM, Kumar A, Moussa NA (2012) Low-level laser therapy: a useful technique for enhancing the proliferation of various cultured cells. Lasers Med Sci 27:237–249CrossRefPubMed

Ohshiro T, Calderhead RG (1988) Low-level laser therapy: a practical introduction. Wiley, New York

Huang YY, Aaron CHC, Carroll JD, Hamblin MR (2009) Biphasic dose response in low-level light therapy. Dose Response 7:358–383CrossRefPubMedPubMedCentral

10.

Yu HS, Chang KL, Yu CL, Chen JW, Chen GS (1996) Low-energy helium-neon laser irradiation stimulates interleukin-1 alpha and interleukin-8 release from cultured human keratinocytes. J Invest Dermatol 107:593–596CrossRefPubMed

11.

Bibikova A, Oron U (1993) Promotion of muscle regeneration in the toad (Bufo viridis) gastrocnemius muscle by low-energy laser irradiation. Anat Rec 235:374–380CrossRefPubMed

12.

Conlan MJ, Rapley JW, Cobb CM (1996) Biostimulation of wound healing by low-energy laser irradiation, a review. J Clin Periodontol 23:492–496CrossRefPubMed

13.

Hrnjak M, Kuljic-Kapulica N, Budisin A, Giser A (1995) Stimulatory effect of low-power density He-Ne laser radiation on human fibroblasts in vitro. Vojnosanit Pregl 52:539–546PubMed

14.

Havel M, Betz CS, Leunig A, Sroka R (2014) Diode laser-induced tissue effects: in vitro tissue model study and in vivo evaluation of wound healing following non-contact application. Lasers Surg Med 46:449–455CrossRefPubMed

15.

Gál P, Mokrý M, Vidinský M, Kilík R, Depta F, Harakaľová M, Longauer F, Mozeš Š, Sabo J (2009) Effect of equal daily doses achieved by different power densities of low-level laser therapy at 635 nm on open skin wound healing in normal and corticosteroid-treated rats. Lasers Med Sci 24:539–547CrossRefPubMed

16.

Nascimento PM, Pinheiro AL, Salgado MA, Ramalho LM (2004) A preliminary report on the effect of laser therapy on the healing of cutaneous surgical wounds as a consequence of an inversely proportional relationship between wavelength and intensity: histological study in rats. Photomed Laser Surg 22:513–518. doi:10.1089/pho.2004.22.513 CrossRefPubMed

17.

Karu TI, Afanasyeva NI, Kolyakov SF, Pyatibrat LV, Welser L (2001) Changes in absorbance of monolayer of living cells induced by laser radiation at 633, 670 and 820 nm. IEEE J Quantum Electron 7:982–988. doi:10.1109/2944.983303 CrossRef

18.

Sanati MH, Torkaman G, Hedayati M, Dizaji MM (2011) Effect of Ga–As (904 nm) and He–Ne (632.8 nm) laser on injury potential of skin full-thickness wound. J Photochem Photobiol B 103(2011):180–185CrossRefPubMed

19.

Hussein AJ, Alfars AA, Falih MAJ, Hassan A-NA (2011) Effects of a low level laser on the acceleration of wound healing in rabbits. North Am J Med Sci 3:193–197CrossRef

20.

Basso FG, Oliveira CF, Kurachi C, Hebling J, Costa CAS (2013) Biostimulatory effect of low-level laser therapy on keratinocytes in vitro. Lasers Med Sci 28:367–374CrossRefPubMed

21.

Basso FG, Pansani TN, Turrioni APS, Bagnato VS, Hebling J, Costa CAS (2012) In vitrowound healing improvement by low-level laser therapy application in cultured gingival fibroblasts. Hindawi Publishing Corporation Int J Dent 2012:719452

22.

Dean DA, Ramanathan T, Machado D, Sundararajan R (2008) Electrical impedance spectroscopy study of biological tissues. J Electrostat 66(3–4):165–177CrossRefPubMedPubMedCentral

23.

McRae D, Esrick M, Mueller S (1999) Changes in the noninvasive, in vivo electrical impedance of three xenografts during the necrotic cell-response sequence. Int J Radiat Oncol 43(4):849–857CrossRef

24.

Soleya A, Lecinaa M, Gámeza X, Cairóa JJ, Riub P, Rosellb X, Bragósb R, Gòdiaa F (2005) On-line monitoring of yeast cell growth by impedance spectroscopy. J Biotechnol 118:398–405CrossRef

25.

Solmaz H, Ülgen Y, Tümer M (2009) Design of a microcontroller based Cole-Cole impedance meter for testing biological tissues. WC 2009, IFMBE Proceedings 25/VII:488–491

26.

Yang Y, Wang J, Yu G, Niu F, He P (2006) Design and preliminary evaluation of a portable device for the measurement of bioimpedance spectroscopy. Physiol Meas 27:1293–1310CrossRefPubMed

27.

Henry CL, Moore M (2012) Bioelectrical impedance assessment of wound healing. J Diabetes Sci Technol 6(1):209–212CrossRef

28.

Kyle UG, Bosaeus I, Lorenzo AD, Deurenberg P, Elia GJM, Heitmann BL, Smith LK, Melchior JC, Pirlich M, Scharfetter H, Schols AMWJ, Pichard C (2004) Bioelectrical impedance analysis—part I: review of principles and methods. Clin Nutr 23:1226–1243CrossRefPubMed

29.

Tanasait N, Tomomi H, Kazunori K, Hiroshi Y, Junichi K, Isao A (2002) Changes in electrophysiological properties of rat skin with age. Biol Pharm Bull 25(9):1192–1196CrossRef

30.

Kun S, Ristic B, Peura RA, Dunn RM (2003) Algorithm for tissue ischemia estimation based on electrical impedance spectroscopy. IEEE Trans Biomed Eng 50:1352–1359CrossRefPubMed

31.

Mayer M, Brunner P, Merwa R, Scharfetter H (2005) Monitoring of lung edema using focused impedance spectroscopy: a feasibility study. Physiol Meas 26:185–192CrossRefPubMed

32.

Aberg P, Nicander I, Hansson J, Geladi P, Holmgren U, Ollmar S (2004) Skin cancer identification using multifrequency electrical impedance—a potential screening tool. IEEE Trans Biomed Eng 51:2097–2102CrossRefPubMed

33.

Norman K, Stobaus N, Zocher D, Bosy-Westphal A, Szramke A, Scheufele R, Smoliner C, Pirlich M (2010) Cutoff percentiles of bioelectrical phase angle predict functionality, quality of life, and mortality in patients with cancer. Am J Clin Nutr 92:612–619CrossRefPubMed

34.

Paiva SI, Borges LR, Halpern-Silveira D, Assuncao CF, Barros AJD, Gonzalez MC (2011) Standardized phase angle from bioelectrical impedance analysis as prognostic factor for survival in patients with cancer. Support Care Cancer 19:187–192CrossRef

35.

Pliquett U, Prausnitz M (2000) Impedance spectroscopy for rapid and noninvasive analysis of skin electroporation. electrochemotherapy, electrogenetherapy and transdermal delivery. Humana Press, New Jersey, pp 377–406CrossRef

36.

Osterman KS (2004) Non-invasive assessment of radiation injury with electrical impedance spectroscopy. Phys Med Biol 49:665–683CrossRefPubMed

37.

Bauchot AD, Harker FR, Arnold WM (2000) The use of electrical impedance spectroscopy to assess the physiological condition of kiwifruit. Postharvest Biotechnol Technol 18:9–18CrossRef

38.

Palko T, Bialokoz F, Weglarz J (2011) Multifrequency device for measurement of the complex electrical bioimpedance—design and application. Proceedings RC IEEE-EMBS & 14th BMESI.

39.

Steendijk P, Mur G, Van Der Velde ET, Baan J (1993) The four electrode resistivity technique in anisotropic media: theoretical analysis and application on myocardial tissue in vivo. IEEE Trans Biomed Eng 40(11):1138–1148CrossRefPubMed

40.

Baum CL, Christopher JA (2005) Normal cutaneous wound healing: clinical correlation with cellular and molecular events. Dermatol Surg 31:674–686CrossRefPubMed

41.

Bjorklund S, Ruzgas T, Nowacka A, Dahi I, Topgaard D, Sparr E, Engblom J (2013) Skin membrane electrical impedance properties under the influence of a varying water gradient. Biophys J 104:2639–2650CrossRefPubMedPubMedCentral

42.

Warren DS, Pomeranz B (1996) Surgical wound healing monitored repeatedly in vivo using electrical resistance of the epidermis. Physiol Meas 17:57–69CrossRef

Title: Laser biostimulation of wound healing: bioimpedance measurements support histology
Authors: Hakan Solmaz
Sergulen Dervisoglu
Murat Gulsoy
Yekta Ulgen
Publication date: 01-11-2016
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 8/2016
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-016-2013-9

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Laser biostimulation of wound healing: bioimpedance measurements support histology

Abstract

Keynote webinar | Spotlight on medication adherence

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 8/2016

The picosecond laser for tattoo removal

Improving the outcome of fractional CO2 laser resurfacing using a probiotic skin cream: Preliminary clinical evaluation

Comment on “Effect of low-level phototherapy on delayed onset muscle soreness: a systematic review and meta-analysis”

Wound healing efficacy of a 660-nm diode laser in a rat incisional wound model

Reporting quality of randomized controlled trial abstracts published in leading laser medicine journals: an assessment using the CONSORT for abstracts guidelines

Treatment of nasal ala nodular congenital melanocytic naevus with carbon dioxide laser and Q-switched Nd:YAG laser