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01-02-2015 | Original Article

Effect of optical clearing agents on optical coherence tomography images of cervical epithelium

Authors: Julia Gallwas, Anna Stanchi, Nina Ditsch, Theresa Schwarz, Christian Dannecker, Susanna Mueller, Herbert Stepp, Uwe Mortensen

Published in: Lasers in Medical Science | Issue 2/2015

Abstract

Optical coherence tomography (OCT) can be used as an adjunct to colposcopy in order to detect precancerous and cancerous cervical lesions. Optical clearing agents (OCAs) temporarily reduce the optical scattering of biological tissues. The purpose of this study was to investigate their influence on OCT imaging. OCT images were taken from unsuspicious and suspicious areas of fresh conization specimens immediately after resection and 5, 10, and 20 min after application of dimethyl sulfoxide (DMSO) or polyethylene glycol (PEG). Corresponding histologies were obtained from all sites. The images taken 5, 10, and 20 min after application of OCA were compared to the initial images with respect to changes in brightness, contrast, and scanning depth using a standard nonparametric test of differences of proportions. Further, mean intensity backscattering curves were calculated from all OCT images in the histological groups CIN2, CIN3, inflammation, and normal epithelium. Mean difference profiles within each of these groups were determined, reflecting the mean differences between the condition before the application of OCA and the exposure times 5, 10, and 20 min, respectively. The null hypothesis was tested employing the Dicky-Fuller-test, Hotelings-test and run test. The visual analysis of 434 OCT images from 109 different sites of 24 conization specimens showed a statistically significant increase in brightness and contrast for normal and dysplastic epithelium after application of DMSO or PEG. Further, the analysis of mean intensity profiles suggests the existence of an increased backscattering intensity after application of DMSO or PEG. DMSO and PEG contribute substantially to optical clearing in cervical squamous epithelium and therefore influence OCT imaging in a positive way. With further refinement of the OCT technology, the observed changes may be beneficial in interpreting the tissue microstructure and identifying cervical intraepithelial neoplasia.

Turchin IV, Sergeeva EA, Dolin LS, Kamensky VA, Shakhova NM, Richards-Kortum R (2005) Novel algorithm of processing optical coherence tomography images for differentiation of biological tissue pathologies. J Biomed Opt 10, 064024PubMedCrossRef

Bazant-Hegemark F, Edey K, Swingler GR, Read MD, Stone N (2008) Review: optical micrometer resolution scanning for non-invasive grading of precancer in the human uterine cervix. Technol Cancer Res Treat 7(6):483–496, ReviewPubMedCrossRef

Escobar PF, Rojas-Espaillat L, Tisci S, Enerson C, Brainard J, Smith J, Tresser NJ, Feldchtein FI, Rojas LB, Belinson JL (2006) Optical coherence tomography as a diagnostic aid to visual inspection and colposcopy for preinvasive and invasive cancer of the uterine cervix. Int J Gynecol Cancer 16:1815–1822PubMedCrossRef

Gallwas JMD, Turk L, Friese K, Dannecker C (2010) Optical coherence tomography (OCT) as a non invasive imaging technique for preinvasive and invasive neoplasia of the uterine cervix. Ultrasound Obstet Gynecol 36(5):624–629PubMedCrossRef

Kang W, Qi X, Tresser NJ, Kareta M, Belinson JL, Rollins AM (2011) Diagnostic efficacy of computer extracted image features in optical coherence tomography of the precancerous cervix. Med Phys 38(1):107–113PubMedCentralPubMedCrossRef

Gallwas J, Turk L, Stepp H, Mueller S, Ochsenkuehn R, Friese K, Dannecker C (2011) Optical coherence tomography for the diagnosis of cervical intraepithelial neoplasia. Lasers Surg Med 43(3):206–212PubMedCrossRef

He Y, Wang RK (2004) Dynamic optical clearing effect of tissue impregnated with hyperosmotic agents and studied with optical coherence tomography. J Biomed Opt 9(1):200–206PubMedCrossRef

Rylander CG, Stumpp OF, Milner TE, Kemp NJ, Mendenhall JM, Diller KR, Welch AJ (2006) Dehydration mechanism of optical clearing in tissue. J Biomed Opt 11(4), 041117PubMedCrossRef

Millon SR, Roldan-Perez KM, Riching KM, Palmer GM, Ramanujam N (2006) Effect of optical clearing agents on the in vivo optical properties of squamous epithelial tissue. Lasers Surg Med 38(10):920–927PubMedCrossRef

10.

Zhu Z, Wu G, Wei H, Yang H, He Y, Xie S, Zhao Q, Guo X (2012) Investigation of the permeability and optical clearing ability of different analytes in human normal and cancerous breast tissues by spectral domain OCT. J Biophotonics 5(7):536–543PubMedCrossRef

11.

Zhu D, Larin KV, Luo Q, Tuchin VV (2013) Recent progress in tissue optical clearing. Laser Photonics Rev 7(5):732–757CrossRef

12.

Genina EA, Bashkatov AN, Kolesnikova EA, Basko MV, Terentyuk GS, Tuchin VV (2014) Optical coherence tomography monitoring of enhanced skin optical clearing in rats in vivo. J Biomed Opt 19(2):21109PubMedCrossRef

13.

Fleiss JL, Levin B, Paik MC (2003) Statistical methods for rates and proportions, 3rd edn. Wiley, New YorkCrossRef

14.

Trapletti A, Hornik K, LeBaron B (2013) Package ‘tseries’ http://www.R-project.org/

15.

R core Team (2003) R: a language and environment for statistical computing. R Foundation for Statistical Computing, Vienna, URL http://www.R-project.org/

16.

Said E, David A (1984) Dickey: testing for unit roots in autoregressive moving average models of unknown order. Biometrika 71:599–607CrossRef

17.

Gallwas J, Stanchi A, Dannecker C, Ditsch N, Mueller S, Mortensen U, Stepp H (2014) Effect of acetic acid on optical coherence tomography (OCT) images of cervical epithelium. Lasers Med Sci 2014 May 15

18.

Gallwas J, Mortensen U, Gaschler R, Ochsenkuehn R, Stepp H, Friese K, Dannecker C (2012) Validation of an ex vivo human cervical tissue model for optical imaging studies. Lasers Surg Med 44(3):245–248PubMedCrossRef

19.

Hiller C, Bock U, Balser S, Haltner-Ukomadu E, Dahm M (2008) Establishment and validation of an ex vivo human cervical tissue model for local delivery studies. Eur J Pharm Biopharm 68(2):390–399PubMedCrossRef

Title: Effect of optical clearing agents on optical coherence tomography images of cervical epithelium
Authors: Julia Gallwas
Anna Stanchi
Nina Ditsch
Theresa Schwarz
Christian Dannecker
Susanna Mueller
Herbert Stepp
Uwe Mortensen
Publication date: 01-02-2015
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 2/2015
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1674-5

At a glance: The STEP trials

Springer Medicine

Effect of optical clearing agents on optical coherence tomography images of cervical epithelium

Abstract

At a glance: The STEP trials

Springer Medicine

Abstract

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