Top

Published in:

01-11-2014 | Original Article

Effect of low-level laser therapy on repair of the bone compromised by radiotherapy

Authors: Jonas D. Batista, Darceny Zanetta-Barbosa, Sérgio V. Cardoso, Paula Dechichi, Flaviana S. Rocha, Rogério M. Pagnoncelli

Published in: Lasers in Medical Science | Issue 6/2014

Abstract

Radiotherapy (RDT) is commonly used for cancer treatment, but high doses of ionizing radiation can directly affect healthy tissues. Positive biological effects of low-level laser therapy (LLLT) on bone repair have been demonstrated; however, this effect on surgical defects of bone previously compromised by radiotherapy has not been evaluated. The aim of this study was to investigate the influence of LLLT (λ = 830 nm) in femur repair after ionizing radiation. Twenty Wistar rats were divided into four groups: control group (GC, n = 5) creation of bone defects (BDs) only; laser group (GL), with BD and LLLT (n = 5); radiotherapy group (GR), submitted to RDT and BD (n = 5); and radiotherapy and laser group (GRL), submitted to RDT, BD, and LLLT (n = 5). GL and GRL received punctual laser application (DE = 210 J/cm², P = 50 mW, t = 120 s, and beam diameter of 0.04 cm²) immediately after surgery, with 48-h interval during 7 days. Animals were euthanized at 7 days after surgery, and bone sections were evaluated morphometrically with conventional microscopy. Bone repair was only observed in nonirradiated bone, with significant improvement in GL in comparison to GC. GR and GRL did not present any bone neoformation. The result demonstrated a positive local biostimulative effect of LLLT in normal bone. However, LLLT was not able to revert the bone metabolic damage due to ionizing radiation.

Stone HB, Coleman CN, Anscher MS, McBride WH (2003) Effects of radiation on normal tissue: consequences and mechanisms. Lancet Oncol 4:529–536PubMedCrossRef

Zhuang Q, Zhang Z, Fu H, He J, He Y (2011) Does radiation-induced fibrosis have an important role in pathophysiology of the osteoradionecrosis of jaw? Med Hypotheses 77:63–65PubMedCrossRef

Rabelo GD, Beletti ME, Dechichi P (2010) Histological analysis of the alterations on cortical bone channels network after radiotherapy: a rabbit study. Microsc Res Tech 73:1015–1018PubMedCrossRef

Da Cunha SS, Sarmento V, Ramalho LM, De Almeida D, Veeck EB, Da Costa NP, Mattos A, Marques AM, Gerbi M, Freitas AC (2007) Effect of laser therapy on bone tissue submitted to radiotherapy: experimental study in rats. Photomed Laser Surg 25:197–204PubMedCrossRef

Monnier Y, Broome M, Betz M, Bouferrache K, Ozsahin M, Jaques B (2011) Mandibular osteoradionecrosis in squamous cell carcinoma of the oral cavity and oropharynx: incidence and risk factors. Otolaryngol Head Neck Surg 144:726–732PubMedCrossRef

Lerouxel E, Moreau A, Bouler JM, Giumelli B, Daculsi G, Weiss P, Malard O (2009) Effects of high doses of ionising radiation on bone in rats: a new model for evaluation of bone engineering. Br J Oral Maxillofac Surg 47:602–607PubMedCrossRef

Maeda M, Bryant MH, Yamagata M, Li G, Earle JD, Chao EY (1988) Effects of irradiation on cortical bone and their time-related changes. A biomechanical and histomorphological study. J Bone Joint Surg Am 70:392–399PubMed

Németh Z, Somogyi A, Takácsi-Nagy Z, Barabás J, Németh G, Szabó G (2000) Possibilities of preventing osteoradionecrosis during complex therapy of tumors of the oral cavity. Pathol Oncol Res 6:53–58PubMedCrossRef

Chopra S, Kamdar D, Tulunay Ugur OE, Chen G, Peshek B, Marunick M, Kim H, Lin HS, Jacobs J (2011) Factors predictive of severity of osteoradionecrosis of the mandible. Head Neck 33:1600–1605PubMedCrossRef

10.

Fritz GW, Gunsolley JC, Abubaker O, Laskin DM (2010) Efficacy of pre- and postirradiation hyperbaric oxygen therapy in the prevention of postextraction osteoradionecrosis: a systematic review. J Oral Maxillofac Surg 68:2653–2660PubMedCrossRef

11.

Lehner B, Bauer J, Rödel F, Grabenbauer G, Neukam FW, Schultze-Mosgau S (2004) Radiation-induced impairment of osseous healing with vascularized bone transfer: experimental model using a pedicled tibia flap in rat. Int J Oral Maxillofac Surg 33:486–492PubMedCrossRef

12.

López-Arcas JM, Arias J, Del Castillo JL, Burgueño M, Navarro I, Morán MJ, Chamorro M, Martorell V (2010) The fibula osteomyocutaneous flap for mandible reconstruction: a 15-year experience. J Oral Maxillofac Surg 68:2377–2384PubMedCrossRef

13.

Mooren RE, Merkx MA, Kessler PA, Jansen JA, Stoelinga PJ (2010) Reconstruction of the mandible using preshaped 2.3-mm titanium plates, autogenous cortical bone plates, particulate cancellous bone, and platelet-rich plasma: a retrospective analysis of 20 patients. J Oral Maxillofac Surg 68:2459–2467PubMedCrossRef

14.

Lino MD, Carvalho FB, Oliveira LR, Magalhães EB, Pinheiro AL, Ramalho LM (2011) Laser phototherapy as a treatment for radiotherapy-induced oral mucositis. Braz Dent J 22:162–165PubMed

15.

Ramalho KM, Luiz AC, de Paula EC, Tunér J, Magalhães RP, Gallottini MM (2011) Use of laser phototherapy on a delayed wound healing of oral mucosa previously submitted to radiotherapy: case report. Int Wound J 8:413–418PubMedCrossRef

16.

Akyol UK, Güngörmüş M (2010) Effect of biostimulation on healing of bone defects in diabetic rats. Photomed Laser Surg 28:411–416PubMedCrossRef

17.

Fernandes KR, Ribeiro DA, Rodrigues NC, Tim C, Santos AA, Parizotto NA, de Araujo HS, Driusso P, Rennó AC (2013) Effects of low-level laser therapy on the expression of osteogenic genes related in the initial stages of bone defects in rats. J Biomed Opt 18:038002PubMedCrossRef

18.

Schmitt G, Glaeser L, Kotsch E (1978) Advantages and disadvantages of a 5.7 MeV linear accelerator compared to a 60Co unit. Strahlentherapie 154:257–260PubMed

19.

Jegoux F, Malard O, Goyenvalle E, Aguado E, Daculsi G (2010) Radiation effects on bone healing and reconstruction: interpretation of the literature. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 109:173–184PubMedCrossRef

20.

Hopewell JW (2003) Radiation-therapy effects on bone density. Med Pediatr Oncol 41:208–211PubMedCrossRef

21.

Bentzen SM (2006) Preventing or reducing late side effects of radiation therapy: radiobiology meets molecular pathology. Nat Rev Cancer 6:702–713PubMedCrossRef

22.

Dare A, Hachisu R, Yamaguchi A, Yokose S, Yoshiki S, Okano T (1997) Effects of ionizing radiation on proliferation and differentiation of osteoblast-like cells. J Dent Res 76:658–664PubMedCrossRef

23.

Muhonen A, Haaparanta M, Grönroos T, Bergman J, Knuuti J, Hinkka S, Happonen RP (2004) Osteoblastic activity and neoangiogenesis in distracted bone of irradiated rabbit mandible with or without hyperbaric oxygen treatment. Int J Oral Maxillofac Surg 33:173–178PubMedCrossRef

24.

Hübner W, Blume A, Pushnjakova R, Dekhtyar Y, Hein HJ (2005) The influence of X-ray radiation on the mineral/organic matrix interaction of bone tissue: an FT-IR microscopic investigation. Int J Artif Organs 281:66–73

25.

Wernle JD, Damron TA, Allen MJ, Mann KA (2010) Local irradiation alters bone morphology and increases bone fragility in a mouse model. J Biomech 19:2738–2746CrossRef

26.

Gál P, Vidinský B, Toporcer T, Mokrý M, Mozes S, Longauer F, Sabo J (2006) Histological assessment of the effect of laser irradiation on skin wound healing in rats. Photomed Laser Surg 24:480–488PubMedCrossRef

27.

Garavello-Freitas I, Baranauskas V, Joazeiro PP, Padovani CR, Dal Pai-Silva M, da Cruz-Höfling MA (2003) Low-power laser irradiation improves histomorphometrical parameters and bone matrix organization during tibia wound healing in rats. J Photochem Photobiol B 70:81–89PubMedCrossRef

28.

Pinheiro AL, Gerbi ME (2006) Photoengineering of bone repair processes. Photomed Laser Surg 24:169–178PubMedCrossRef

29.

da Silva RV, Camilli JA (2006) Repair of bone defects treated with autogenous bone graft and low-power laser. J Craniofac Surg 17:297–301PubMedCrossRef

30.

Weber JB, Pinheiro AL, de Oliveira MG, Oliveira FA, Ramalho LM (2006) Laser therapy improves healing of bone defects submitted to autologous bone graft. Photomed Laser Surg 24:38–44PubMedCrossRef

31.

Saito S, Shimizu N (1997) Stimulatory effects of low-power laser irradiation on bone regeneration in midpalatal suture during expansion in the rat. Am J Orthod Dentofac Orthop 111:525–532CrossRef

32.

Gerbi ME, Pinheiro AL, Marzola C, Limeira Júnior Fde A, Ramalho LM, Ponzi EA, Soares AO, Carvalho LC, Lima HV, Gonçalves TO (2005) Assessment of bone repair associated with the use of organic bovine bone and membrane irradiated at 830 nm. Photomed Laser Surg 23:382–388PubMedCrossRef

33.

Pinheiro AL, Martinez Gerbi ME, de Assis Limeira F Jr, Carneiro Ponzi EA, Marques AM, Carvalho CM, de Carneiro Santos R, Oliveira PC, Nóia M, Ramalho LM (2009) Bone repair following bone grafting hydroxyapatite guided bone regeneration and infra-red laser photobiomodulation: a histological study in a rodent model. Lasers Med Sci 24:234–240PubMedCrossRef

34.

Fávaro-Pípi E, Feitosa SM, Ribeiro DA, Bossini P, Oliveira P, Parizotto NA, Renno AC (2010) Comparative study of the effects of low-intensity pulsed ultrasound and low-level laser therapy on bone defects in tibias of rats. Lasers Med Sci 25:727–732PubMedCrossRef

35.

Fávaro-Pípi E, Ribeiro DA, Ribeiro JU, Bossini P, Oliveira P, Parizotto NA, Tim C, de Araújo HS, Renno AC (2011) Low-level laser therapy induces differential expression of osteogenic genes during bone repair in rats. Photomed Laser Surg 29:311–317PubMedCrossRef

36.

Gao X, Xing D (2009) Molecular mechanisms of cell proliferation induced by low power laser irradiation. J Biomed Sci 12:4–20CrossRef

37.

Schartinger VH, Galvan O, Riechelmann H, Dudás J (2012) Differential responses of fibroblasts, non-neoplastic epithelial cells, and oral carcinoma cells to low-level laser therapy. Support Care Cancer 20:523–529PubMedCrossRef

Title: Effect of low-level laser therapy on repair of the bone compromised by radiotherapy
Authors: Jonas D. Batista
Darceny Zanetta-Barbosa
Sérgio V. Cardoso
Paula Dechichi
Flaviana S. Rocha
Rogério M. Pagnoncelli
Publication date: 01-11-2014
Publisher: Springer London
Published in: Lasers in Medical Science / Issue 6/2014
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-014-1602-8

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Effect of low-level laser therapy on repair of the bone compromised by radiotherapy

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 6/2014

Effects of low-level laser therapy on joint pain, synovitis, anabolic, and catabolic factors in a progressive osteoarthritis rabbit model

The effects of low-intensity laser therapy on hepatic ischemia-reperfusion injury in a rat model

Ultrashort pulsed laser (USPL) application in dentistry: basic investigations of ablation rates and thresholds on oral hard tissue and restorative materials

Prevention of dentine caries using silver diamine fluoride application followed by Er:YAG laser irradiation: an in vitro study

Mechanical properties of thin films of laser-welded titanium and their associated welding defects

In vitro effectiveness of antimicrobial photodynamic therapy (APDT) using a 660 nm laser and malachite green dye in Staphylococcus aureus biofilms arranged on compact and cancellous bone specimens