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01-01-2012 | Original Article

Effects of the Er,Cr:YSGG laser on bone and soft tissue in a rat model

Authors: Livia Rodrigues Perussi, Chaine Pavone, Guilherme José Pimentel Lopes de Oliveira, Paulo Sérgio Cerri, Rosemary Adriana Chierici Marcantonio

Published in: Lasers in Medical Science | Issue 1/2012

Abstract

The purpose of this study was to investigate the histological changes that occur in rat soft and hard tissues after Er,Cr:YSGG laser surgery. Each of 20 rats was submitted to four procedures which were randomly distributed to the right and left sides of the animal: procedure 1 dorsal incision with a scalpel; procedure 2 dorsal incision with a 2.0-W Er,Cr:YSGG laser; procedure 3 skull defect created with a diamond bur; procedure 4 skull defect created with a 3.0-W Er,Cr:YSGG laser. The animals were killed 3, 7, 15 and 30 days after surgery, and histological examinations were performed. The histometric analysis of the bone defects was evaluated using an unpaired t-test. Initially, the dorsum showed more histological signs of repair following procedure 1, although similar healing responses following procedures 1 and 2 were seen on day 30 after surgery. By day 30 the bone formation observed following procedure 4 was much more evident than following procedure 3. The unpaired t-test identified significant differences in bone formation on day 30 (p = 0.01), whereas a greater bone percentage was seen following procedure 4 than following procedure 3 (79.96 ± 10.30% and 58.23 ± 9.99%, respectively). Thus, histological repair of the Er,Cr:YSGG laser wounds was similar to that of the scalpel wounds. However, skull defects created with the Er,Cr:YSGG laser showed greater bone formation than defects created with the bur. Within the limitations of this study, we can conclude that the Er,Cr:YSGG laser is a promising surgical instrument in vivo, particularly for bone surgery.

Salmos J, Gerbi ME, Braz R, Andrade ES, Vasconcelos BC, Bessa-Nogueira RV (2010) Methodological quality of systematic reviews analyzing the use of laser therapy in restorative dentistry. Lasers Med Sci 25:127–136PubMedCrossRef

Aoki A, Sasaki KM, Watanabe H, Ishikawa I (2004) Lasers in nonsurgical periodontal therapy. Periodontol 2000 36:59–97PubMedCrossRef

D’Arcangelo C, Di Maio FN, Prosperi GD, Conte E, Baldi M, Caputi S (2007) A preliminary study of healing of diode laser versus scalpel incisions in rat oral tissue: a comparison of clinical, histological, and immunohistochemical results. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 103:764–773PubMedCrossRef

Wang X, Zhang C, Matsumoto K (2005) In vivo study of the healing processes that occur in the jaws of rabbits following perforation by an Er,Cr:YSGG laser. Lasers Med Sci 20:21–27PubMedCrossRef

Schwarz F, Sculean A, Georg T, Reich E (2001) Periodontal treatment with an Er:YAG laser compared to scaling and root planing. A controlled clinical study. J Periodontol 72:361–367PubMedCrossRef

Folwaczny M, Mehl A, Aggstaller H, Hickel R (2002) Antimicrobial effects of 2,94 micron Er:YAG laser radiation on root surfaces: an in vitro study. J Clin Periodontol 29:73–78PubMedCrossRef

Wang X, Ishizaki N, Suzuki N, Kimura Y, Matsumoto K (2002) Morphological changes of bovine mandibular bone irradiated by Er,Cr:YSGG laser: an in vitro study. J Clin Laser Med Surg 20:245–250PubMedCrossRef

Arashiro DS, Rapley JW, Cobb CM, Killoy WJ (1996) Histologic evaluation of porcine skin incisions produced by CO2 laser electrosurgery and scalpel. Int J Periodontics Restor Dent 16:479–491

Lewandrowski KU, Lorente C, Schomacker KT, Flotte TJ, Wilkes JW, Deutsch TF (1996) Use of the Er:YAG laser for improved plating in maxillofacial surgery: comparison of bone healing in laser and drill osteotomies. Lasers Surg Med 19:40–45PubMedCrossRef

10.

Kimura Y, Yu D, Fujita A, Yamashita A, Murakami Y, Matsumoto K (2001) Effects of erbium,chromium:YSGG laser irradiation on canine mandibular bone. J Periodontol 72:1178–1182PubMedCrossRef

11.

Ekworapoj P, Sidhu SK, McCabe JF (2007) Effect of different power parameters of Er,Cr:YSGG laser on human dentine. Lasers Surg Med 22:175–182CrossRef

12.

Cercadillo-Ibarguren I, España-Tost A, Arnabat-Domínguez J, Valmaseda-Castellón E, Berini-Aytés L, Gay-Escoda C (2010) Histologic evaluation of thermal damage produced on soft tissues by CO2, Er,Cr:YSGG and diode lasers. Med Oral Patol Oral Cir Bucal 15:912–918CrossRef

13.

Food and Drug Administration (2001) 510(k). Summary of Safety and Effectiveness Information: Waterlase Millennium, surgical laser instrument. Available at: http://www.accessdata.fda.gov/cdrh_docs/pdf/k011041.pdf. Accessed 20 Apr 2011

14.

Food and Drug Administration (2002) Summary of Safety and Effectiveness Information: Waterlase Millennium, hydrokinetic tissue cutting system. Available at: http://www.accessdata.fda.gov/cdrh_docs/pdf/k013908.pdf. Accessed 20 Apr 2011

15.

Noori ZT, Fekrazad R, Eslami B, Etemadi A, Khosravi S, Mir M (2008) Comparing the effects of root surface scaling with ultrasound instruments and Er,Cr:YSGG laser. Lasers Med Sci 23:283–287PubMedCrossRef

16.

Olivi G, Angiero F, Benedicenti S, Iara G, Signore A, Kaitsas V (2010) Use of the erbium, chromium:yttrium-scandium-gallium-garnet laser on human enamel tissues. Influence of the air-water spray on the laser-tissue interaction: scanning electron microscope evaluations. Lasers Med Sci 25:793–797PubMedCrossRef

17.

Secilmis A, Usumez A, Usumez S, Berk G (2010) Evaluation of mineral content of enamel prepared by erbium, chromium: yttrium-scandium-gallium-garnet laser. Lasers Med Sci 25:467–472PubMedCrossRef

18.

Ting CC, Fukuda M, Watanabe T, Aoki T, Sanaoka A, Noguchi T (2007) Effects of Er,Cr:YSGG laser irradiation on root surface: morphologic analysis and efficiency of calculus removal. J Periodontol 78:2156–2164PubMedCrossRef

19.

Arnabat J, Escribano C, Fenosa A, Vinuesa T, Gay-Escoda C, Berini L, Viñas M (2010) Bactericidal activity of erbium,chromium:yttrium-scandium-gallium-garnet laser in root canals. Lasers Med Sci 25:805–810PubMedCrossRef

20.

Kang HW, Oh J, Welch AJ (2008) Investigations on laser hard tissue ablation under various environments. Phys Med Biol 53:3381–3390PubMedCrossRef

21.

Buchelt M, Kutschera HP, Katterschafka T et al (1994) Er:YAG and Hol:YAG laser osteotomy: the effect of laser ablation on bone healing. Lasers Surg Med 15:373–381PubMedCrossRef

22.

McDavid VG, Cobb CM, Rapley JW, Glaros AG, Spencer P (2001) Laser irradiation of bone: III. Long-term healing following treatment by CO2 and Nd:YAG lasers. J Periodontol 72:74–182CrossRef

23.

Sinha UK, Gallagher LA (2003) Effects of steel scalpel, ultrasonic scalpel, CO2 laser, and monopolar and bipolar electrosurgery on wound healing in guinea pig oral mucosa. Laryngoscope 113:228–236PubMedCrossRef

24.

Friesen LR, Cobb CM, Rapley JW, Brokman LF, Spencer P (1999) Laser irradiation of bone: II. Healing response following treatment by CO₂ and Nd:YAG lasers. J Periodontol 70:75–83PubMedCrossRef

25.

Nelson JS, Orestein A, Liaw LH, Berns MW (1989) Mid-infrared erbium:YAG laser ablation of bone: the effect of laser osteotomy on bone healing. Lasers Surg Med 9:362–374PubMedCrossRef

26.

Eriksson AR, Albrektsson T (1983) Temperature threshold levels for heat-induced bone tissue injury: a vital-microscopic study in the rabbit. J Prosthet Dent 50:101–107PubMedCrossRef

27.

Kilinc E, Roshkind DM, Antonson SA et al (2009) Thermal safety of Er:YAG and Er,Cr:YSGG lasers in hard tissue removal. Photomed Laser Surg 27:565–570PubMedCrossRef

28.

Blanken J, De Moor RJ, Meire M, Verdaasdonk R (2009) Laser induced explosive vapor and cavitation resulting in effective irrigation of the root canal. Part 1: A visualization study. Lasers Surg Med 41:514–519PubMedCrossRef

29.

Rizoui IM, Eversole LR, Kimmel AI (1996) Effects of an erbium,chromium:yttrium,scandium,gallium,garnet laser on mucocutaneous soft tissues. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 82:386–395CrossRef

30.

Shah UK, Poe DS, Rebeiz EF, Perrault DF, Pankratov MM, Shapshay SM (1996) Erbium laser in middle ear surgery: in vitro and in vivo animal study. Laryngoscope 106:418–422PubMedCrossRef

31.

Lee JH, Heo SJ, Koak JY, Kim SK, Lee SJ, Lee SH (2008) Cellular responses on anodized titanium discs after laser irradiation. Lasers Surg Med 40:738–742PubMedCrossRef

Title: Effects of the Er,Cr:YSGG laser on bone and soft tissue in a rat model
Authors: Livia Rodrigues Perussi
Chaine Pavone
Guilherme José Pimentel Lopes de Oliveira
Paulo Sérgio Cerri
Rosemary Adriana Chierici Marcantonio
Publication date: 01-01-2012
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 1/2012
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-011-0920-3

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Effects of the Er,Cr:YSGG laser on bone and soft tissue in a rat model

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