Top

Published in:

01-05-2010 | Original Article

The role of mast cells in non-ablative laser resurfacing with 1,320 nm neodymium:yttrium–aluminium–garnet laser

Authors: Yingbin Shang, Zhan Wang, Ying Pang, Peng Xi, Qiushi Ren

Published in: Lasers in Medical Science | Issue 3/2010

Abstract

The aim of this study was to investigate the role of mast cells in mechanisms of collagen remodelling induced by non-ablative laser treatment. The dorsal skin of Kunming (KM) mice was exposed to 1,320 nm neodymium–yttrium–aluminium garnet (Nd:YAG) laser weekly for four consecutive weeks. Biopsies were taken 1 h after irradiation and 1 day, 7 days, 14 days, 30 days and 60 days after the first treatment. Skin samples were studied for mast cells, fibroblasts, and type I and III collagen, by toluidine blue, haematoxylin–eosin (HE) and immunohistochemical staining, respectively. The total number of mast cells in the skin of experimental group was significantly greater than that in the control at 1 h, 1 day, 21 days and 60 days after the first treatment (P < 0.05, respectively). At any of the time points studied, the number of degranulated mast cells in the experimental group was significantly higher than in the control (P < 0.01, P < 0.01, P < 0.05, P < 0.01, P < 0.05, P < 0.05, respectively).The number of fibroblasts in the experimental group exhibited a significant increase in comparison with those in control skin at days 7, 21, 30 and 60 after irradiation (P < 0.05, P < 0.01, P < 0.01, P < 0.05, respectively). The amount of type I collagen was significantly higher than in the control from day 21 to day 60 (P < 0.05, P < 0.01, P < 0.01, respectively), and type III collagen showed a marked increase between day 7 and day 60, compared with the control (P < 0.01, P < 0.05, P < 0.01, P < 0.01, respectively). There was a significant positive correlation between the number of fibroblasts and granulated mast cells (r = 0.549, P < 0.01). The amount of type I and III collagen also showed significant positive correlations with the number of degranulated mast cells (r = 0.555, P < 0.01 and r = 0.579, P < 0.01, respectively). The results suggested that dermal mast cells might be involved in the inflammatory response, fibroblast proliferation and collagen remodelling induced by non-ablative laser treatment.

Alam M, Hsu TS, Dover JS, Wrone DA, Arndt KA (2003) Nonablative laser and light treatments: histology and tissue effects—a review. Lasers Surg Med 33:30–39CrossRefPubMed

Rokhsar CK, Lee S, Fitzpatrick RE (2005) Review of photorejuvenation: devices, cosmeceuticals, or both? Dermatol Surg 31:1166–1178PubMedCrossRef

Hirsch RJ, Dayan SH (2004) Nonablative resurfacing. Facial Plast Surg 20:57–61CrossRefPubMed

Weiss RA, McDaniel DH, Geronemus RG (2003) Review of nonablative photorejuvenation: reversal of the aging effects of the sun and environmental damage using laser and light sources. Semin Cutan Med Surg 22:93–106CrossRefPubMed

Ross EV, Zelickson BD (2002) Biophysics of nonablative dermal remodeling. Semin Cutan Med Surg 21:251–265CrossRefPubMed

Noli C, Miolo A (2001) The mast cell in wound healing. Vet Dermatol 12:303–313CrossRefPubMed

Artuc M, Hermes B, Steckelings UM, Grützkau A, Henz BM (1999) Mast cells and their mediators in cutaneous wound healing—active participants or innocent bystanders? Exp Dermatol 8:1–16CrossRefPubMed

Iba Y, Shibata A, Kato M, Masukawa T (2004) Possible involvement of mast cells in collagen remodeling in the late phase of cutaneous wound healing in mice. Int Immunopharmacol 4:1873–1880CrossRefPubMed

Goldberg DJ (2000) Full-face nonablative dermal remodeling with a 1320 nm Nd:YAG laser. Dermatol Surg 26:915–918CrossRefPubMed

10.

Fatemi A, Weiss MA, Weiss RA (2002) Short-term histologic effects of nonablative resurfacing: results with a dynamically cooled millisecond-domain 1320 nm Nd:YAG laser. Dermatol Surg 28:172–176CrossRefPubMed

11.

Kelly KM, Nelson JS, Lask GP, Geronemus RG, Bernstein LJ (1999) Cryogen spray cooling in combination with nonablative laser treatment of facial rhytides. Arch Dermatol 135:691–694CrossRefPubMed

12.

Menaker GM, Wrone DA, Williams RM, Moy RL (1999) Treatment of facial rhytids with a nonablative laser: a clinical and histologic study. Dermatol Surg 25:440–444CrossRefPubMed

13.

Goldberg DJ (1999) Non-ablative subsurface remodeling: clinical and histologic evaluation of a 1320-nm Nd:YAG laser. J Cutan Laser Ther 1:153–157PubMed

14.

Trelles MA, Allones I, Luna R (2001) Facial rejuvenation with a nonablative 1320 nm Nd:YAG laser: a preliminary clinical and histologic evaluation. Dermatol Surg 27:111–116CrossRefPubMed

15.

Dang Y, Ren Q, Liu H, Ma J, Zhang J (2006) Effects of the 1, 320-nm Nd:YAG laser on transepidermal water loss, histological changes, and collagen remodeling in skin. Lasers Med Sci 21:147–152CrossRefPubMed

16.

Arck PC, Handjiski B, Peters EM, Peter AS, Hagen E, Fischer A, Klapp BF, Paus R (2003) Stress inhibits hair growth in mice by induction of premature catagen development and deleterious perifollicular inflammatory events via neuropeptide substance P-dependent pathways. Am J Pathol 162:803–814PubMed

17.

Trelles MA, Mordon S, Calderhead RG (2007) Facial rejuvenation and light: our personal experience. Lasers Med Sci 22:93–99CrossRefPubMed

18.

Sadick NS (2003) Update on non-ablative light therapy for rejuvenation: a review. Lasers Surg Med 32:120–128CrossRefPubMed

19.

Lask G, Lee PK, Seyfzadeh M et al (1997) Nonablative laser treatment of facial rhytides. Proc SPIE 2970:338–349CrossRef

20.

Kirsner RS, Eaglstein WH (1993) The wound healing process. Dermatol Clin 11:629–640PubMed

21.

Walsh LJ (2003) Mast cells and oral inflammation. Crit Rev Oral Biol Med 14:188–198CrossRefPubMed

22.

el Sayed SO, Dyson M (1993) Responses of dermal mast cells to injury. J Anat 182:369–376PubMed

23.

Malaviya R, Morrison AR, Pentland AP (1996) Histamine in human epidermal cells is induced by ultraviolet light injury. J Invest Dermatol 106:785–789CrossRefPubMed

24.

Azevedo LH, de Sousa SC, Correa L, de Paula Eduardo C, Dagli ML, Romanos G, Migliari DA (2008) Mast cell concentration in the wound healing process of incisions made by different instruments. Lasers Med Sci (in press)

25.

Trelles MA, Mayayo E, Miro L, Baudin G (1986) Mast cells and low-power laser: analysis of its components before and after irradiation. Lasers Med Sci 1:299

26.

Trabucchi E, Radaelli E, Marazzi M, Foschi D, Musazzi M, Veronesi AM, Montorsi W (1988) The role of mast cells in wound healing. Int J Tissue React 10:367–372PubMed

27.

Levi-Schaffer F, Kupietzky A (1990) Mast cells enhance migration and proliferation of fibroblasts into an in vitro wound. Exp Cell Res 188:42–49CrossRefPubMed

28.

Russel JD, Russell SB, Trupin KM (1977) The effect of histamine on the growth of cultured fibroblasts isolated from normal and keloid tissue. J Cell Physiol 93:389–393CrossRefPubMed

29.

Kupietzky A, Levi-Schaffer F (1996) The role of mast cell-derived histamine in the closure of an in vitro wound. Inflamm Res 45:176–180CrossRefPubMed

30.

Gruber BL, Kew RR, Jelaska A, Marchese MJ, Garlick J, Ren S, Schwartz LB, Korn JH (1997) Human mast cells activate fibroblasts: tryptase is a fibrogenic factor stimulating collagen messenger ribonucleic acid synthesis and fibroblast chemotaxis. J Immunol 158:2310–2317PubMed

31.

Kovacs EJ (1991) Fibrogenic cytokines: the role of immune mediators in the development of scar tissue. Immunol Today 12:17–23CrossRefPubMed

32.

Bressler RB, Lesko J, Jones ML, Wasserman M, Dickason RR, Huston MM, Cook SW, Huston DP (1997) Production of IL-5 and granulocyte-macrophage colony-stimulating factor by naive human mast cells activated by high-affinity IgE receptor ligation. J Allergy Clin Immunol 99:508–514CrossRefPubMed

33.

Qu Z, Kayton RJ, Ahmadi P, Liebler JM, Powers MR, Planck SR, Rosenbaum JT (1998) Ultrastructural immunolocalization of basic fibroblast growth factor in mast cell secretory granules. Morphological evidence for bFGF release through degranulation. J Histochem Cytochem 46:1119–1128PubMed

34.

Metcalfe DD, Baram D, Mekori YA (1997) Mast cells. Physiol Rev 77:1033–1079PubMed

35.

Vliagoftis H, Hutson AM, Mahmudi-Azer S, Kim H, Rumsaeng V, Oh CK, Moqbel R, Metcalfe DD (1999) Mast cells express connexins on their cytoplasmic membrane. J Allergy Clin Immunol 103:656–662CrossRefPubMed

36.

Saarinen J, Kalkkinen N, Welgus HG, Kovanen PT (1994) Activation of human interstitial procollagenase through direct cleavage of the Leu83-Thr84 bond by mast cell chymase. J Biol Chem 269:18134–18140PubMed

37.

Kofford MW, Schwartz LB, Schechter NM, Yager DR, Diegelmann RF, Graham MF (1997) Cleavage of type I procollagen by human mast cell chymase initiates collagen fibril formation and generates a unique carboxyl-terminal propeptide. J Biol Chem 272:7127–7131CrossRefPubMed

38.

Nishikori Y, Kakizoe E, Kobayashi Y, Shimoura K, Okunishi H, Dekio S (1998) Skin mast cell promotion of matrix remodeling in burn wound healing in mice: relevance of chymase. Arch Dermatol Res 290:553–560CrossRefPubMed

39.

Kovacs EJ (1991) Fibrogenic cytokines: the role of immune mediators in the development of scar tissue. Immunol Today 12:17–23CrossRefPubMed

Title: The role of mast cells in non-ablative laser resurfacing with 1,320 nm neodymium:yttrium–aluminium–garnet laser
Authors: Yingbin Shang
Zhan Wang
Ying Pang
Peng Xi
Qiushi Ren
Publication date: 01-05-2010
Publisher: Springer-Verlag
Published in: Lasers in Medical Science / Issue 3/2010
Print ISSN: 0268-8921
Electronic ISSN: 1435-604X
DOI: https://doi.org/10.1007/s10103-009-0703-2

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

The role of mast cells in non-ablative laser resurfacing with 1,320 nm neodymium:yttrium–aluminium–garnet laser

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 3/2010

Calcitonin, sodium alendronate and high intensity laser in the treatment of traumatized teeth: a preliminary study

Influence of etching with erbium, chromium:yttrium–scandium–gallium–garnet laser on microleakage of class V restoration

In vivo comparison of near infrared lasers for skin welding

Effect of laser phototherapy on recurring herpes labialis prevention: an in vivo study

Depth sensitivity analysis of functional near-infrared spectroscopy measurement using three-dimensional Monte Carlo modelling-based magnetic resonance imaging

A feasibility study on laser rapid forming of a complete titanium denture base plate