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Neurological Sciences
Published in: Neurological Sciences 7/2022

19-02-2022 | Muscular Dystrophy | Original Article

Effects of low-intensity training on the brain and muscle in the congenital muscular dystrophy 1D model

Authors: Clarissa M. Comim, Jaime A. Soares, Adriano Alberti, Viviane Freiberger, Letícia Ventura, Paula Dias, Aryadnne L. Schactae, Leoberto R. Grigollo, Amanda V. Steckert, Daniel F. Martins, Rudy J. Nodari Junior, Mariz Vainzof, João Quevedo

Published in: Neurological Sciences | Issue 7/2022

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Abstract

Introduction

Congenital Muscular Dystrophy type 1D (MDC1D) is characterized by a hypoglycosylation of α-dystroglycan protein (α-DG), and this may be strongly implicated in increased skeletal muscle tissue degeneration and abnormal brain development, leading to cognitive impairment. However, the pathophysiology of brain involvement is still unclear. Low-intensity exercise training (LIET) is known to contribute to decreased muscle degeneration in animal models of other forms of progressive muscular dystrophies.

Aim

The objective of this study was to analyze the effects of LIET on cognitive involvement and oxidative stress in brain tissue and gastrocnemius muscle.

Methods

Male homozygous (Largemyd−/−), heterozygous (Largemyd+/−), and wild-type mice were used. To complete 28 days of life, they were subjected to a low-intensity exercise training (LIET) for 8 weeks. After the last day of training, 24 h were expected when the animals were submitted to inhibitory avoidance and open-field test. The striatum, prefrontal cortex, hippocampus, cortex, and gastrocnemius were collected for evaluation of protein carbonylation, lipid peroxidation, and catalase and superoxide dismutase activity.

Results

LIET was observed to reverse the alteration in aversive and habituation memory. Increased protein carbonylation in the striatum, prefrontal cortex, and hippocampus and lipid peroxidation in the prefrontal cortex and hippocampus were also reversed by LIET. In the evaluation of the antioxidant activity, LIET increased catalase activity in the hippocampus and cortex. In the gastrocnemius, LIET decreased the protein carbonylation and lipid peroxidation and increased catalase and superoxide dismutase activity.

Conclusion

In conclusion, it can be inferred that LIET for 8 weeks was able to reverse the cognitive damage and oxidative stress in brain tissue and gastrocnemius muscle in MDC1D animals.
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Metadata
Title
Effects of low-intensity training on the brain and muscle in the congenital muscular dystrophy 1D model
Authors
Clarissa M. Comim
Jaime A. Soares
Adriano Alberti
Viviane Freiberger
Letícia Ventura
Paula Dias
Aryadnne L. Schactae
Leoberto R. Grigollo
Amanda V. Steckert
Daniel F. Martins
Rudy J. Nodari Junior
Mariz Vainzof
João Quevedo
Publication date
19-02-2022
Publisher
Springer International Publishing
Published in
Neurological Sciences / Issue 7/2022
Print ISSN: 1590-1874
Electronic ISSN: 1590-3478
DOI
https://doi.org/10.1007/s10072-022-05928-w

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