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01-09-2017 | Original Article

Evaluation of endothelialization in the center part of graft using 3 cm vascular grafts implanted in the abdominal aortae of the rat

Authors: Toshiharu Fukayama, Yusuke Ozai, Haruka Shimokawatoko, Yusuke Kimura, Derya Aytemiz, Ryou Tanaka, Noboru Machida, Tetsuo Asakura

Published in: Journal of Artificial Organs | Issue 3/2017

Abstract

In order to develop small-diameter vascular grafts, it is necessary to evaluate endothelialization, especially, in the center part at early stage. For implantation of vascular grafts of 1 cm in length to abdominal aortae of rat, endothelial cells can be formed easily by stretching anastomosis. We evaluated the endothelialization in the center part of vascular grafts by implanting vascular grafts using transgenic (TG) silk fibroin (SF) of 3 cm in length. Vascular grafts were prepared 1.5 mm in diameter and 1 and 3 cm in length using wild type (WT) SF and TG SF by braiding structure, respectively. The grafts were removed after 2 weeks or 3 months and evaluated pathologically. Endothelialization was not confirmed totally after 3 months of implantation. However, endothelialization in the center part of grafts was significantly higher in TG SF than in WT SF. No significant difference was found regarding tissue infiltration and internal diameter. The TG SF revealed migration of the endothelial cells into the center part of the vessels at the early stage. Also, tissue infiltration and remodeling is expected using SF. The 3 cm length vascular grafts can be evaluated as a new experimental system.

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Title: Evaluation of endothelialization in the center part of graft using 3 cm vascular grafts implanted in the abdominal aortae of the rat
Authors: Toshiharu Fukayama
Yusuke Ozai
Haruka Shimokawatoko
Yusuke Kimura
Derya Aytemiz
Ryou Tanaka
Noboru Machida
Tetsuo Asakura
Publication date: 01-09-2017
Publisher: Springer Japan
Published in: Journal of Artificial Organs / Issue 3/2017
Print ISSN: 1434-7229
Electronic ISSN: 1619-0904
DOI: https://doi.org/10.1007/s10047-017-0957-6

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Evaluation of endothelialization in the center part of graft using 3 cm vascular grafts implanted in the abdominal aortae of the rat

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

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