Top

Published in:

Open Access 01-06-2017 | Original Article

Visual evoked potential in RCS rats with Okayama University-type retinal prosthesis (OUReP™) implantation

Authors: Alamusi, Toshihiko Matsuo, Osamu Hosoya, Tetsuya Uchida

Published in: Journal of Artificial Organs | Issue 2/2017

Abstract

Photoelectric dye-coupled polyethylene film, designated Okayama University type-retinal prosthesis or OUReP™, generates light-evoked surface electric potentials and stimulates neurons. The dye-coupled films or plain films were implanted subretinally in both eyes of 10 Royal College of Surgeons rats with hereditary retinal dystrophy at the age of 6 weeks. Visual evoked potentials in response to monocular flashing light stimuli were recorded from cranially-fixed electrodes, 4 weeks and 8 weeks after the implantation. After the recording, subretinal film implantation was confirmed histologically in 7 eyes with dye-coupled films and 7 eyes with plain films. The recordings from these 7 eyes in each group were used for statistical analysis. The amplitudes of visual evoked potentials in the consecutive time points from 125 to 250 ms after flash were significantly larger in the 7 eyes with dye-coupled film implantation, compared to the 7 eyes with plain film implantation at 8 weeks after the implantation (P < 0.05, repeated-measure ANOVA). The photoelectric dye-coupled polyethylene film, as retinal prosthesis, gave rise to visual evoked potential in response to flashing light.

Loewenstein JI, Montezuma SR, Rizzo JF. Outer retinal degeneration: an electronic retinal prosthesis as a treatment strategy. Arch Ophthalmol. 2004;122:587–96.CrossRefPubMed

Humayun MS, Dorn JD, da Cruz L, Dagnelie G, Sahel JA, Stanga PE, Cideciyan AV, Duncan JL, Eliott D, Filley E, Ho AC, Santos A, Safran AB, Arditi A, Del Priore LV, Greenberg RJ, Argus II Study Group. Interim results from the international trial of Second Sight’s visual prosthesis. Ophthalmology. 2012;119:779–88.CrossRefPubMedPubMedCentral

Matsuo T. A simple method for screening photoelectric dyes towards their use for retinal prostheses. Acta Med Okayama. 2003;57:257–60.PubMed

Uchida T, Ishimaru S, Shimamura K, Uji A, Matsuo T, Ohtsuki H. Immobilization of photoelectric dye on the polyethylene film surface. Mem Fac Eng Okayama Univ. 2005;39:16–20.

Matsuo T, Dan-oh Y, Suga S (Inventors). Agent for inducing receptor potential. Assignee: Okayama University. United States Patent. Patent No.: US 7,101,533 B2. Date of Patent: Sep. 5, 2006.

Uji A, Matsuo T, Ishimaru S, Kajiura A, Shimamura K, Ohtsuki H, Dan-oh Y, Suga S. Photoelectric dye-coupled polyethylene film as a prototype of retinal prostheses. Aritif Organs. 2005;29:53–7.CrossRef

Uji A, Matsuo T, Uchida T, Shimamura K, Ohtsuki H. Intracellular calcium response and adhesiveness of chick embryonic retinal neurons to photoelectric dye-coupled polyethylene films as prototypes of retinal prostheses. Artif Organs. 2006;30:695–703.CrossRefPubMed

Tamaki T, Matsuo T, Hosoya O, Tsutsui KM, Uchida T, Okamoto K, Uji A, Ohtsuki H. Glial reaction to photoelectric dye-based retinal prostheses implanted in the subretinal space of rats. J Artif Organs. 2008;11:38–44.CrossRefPubMed

Okamoto K, Matsuo T, Tamaki T, Uji A, Ohtsuki H. Short-term biological safety of a photoelectric dye used as a component of retinal prostheses. J Artif Organs. 2008;11:45–51.CrossRefPubMed

10.

Matsuo T, Uchida T, Takarabe K. Safety, efficacy, and quality control of a photoelectric dye-based retinal prosthesis (Okayama University-type retinal prosthesis) as a medical device. J Artif Organs. 2009;12:213–25.CrossRefPubMed

11.

Matsuo T, Morimoto N. Visual acuity and perimacular retinal layers detected by optical coherence tomography in patients with retinitis pigmentosa. Br J Ophthalmol. 2007;91:888–90.CrossRefPubMedPubMedCentral

12.

Tamaki M, Matsuo T. Optical coherence tomographic parameters as objective signs for visual acuity in patients with retinitis pigmentosa, future candidates for retinal prostheses. J Artif Organs 2011;14:140–150. Erratum. 2011;14:385.

13.

Alamusi, Matsuo T, Hosoya O, Tsutsui KM, Uchida T. Behavior tests and immunohistochemical retinal response analyses in RCS rats with subretinal implantation of Okayama-University-type retinal prosthesis. J Artif Organs. 2013;16:343–51.CrossRefPubMed

14.

Alamusi, Matsuo T, Hosoya O, Tsutsui KM, Uchida T. Vision maintenance and retinal apoptosis reduction in RCS rats with Okayama University-type retinal prosthesis (OUReP™) implantation. J Artif Organs. 2015;18:264–71.CrossRefPubMed

15.

Dowling JE, Sidman RL. Inherited retinal dystrophy in the rat. J Cell Biol. 1962;14:73–109.CrossRefPubMedPubMedCentral

16.

Papathanasiou ES, Peachey NS, Goto Y, Neafsey EJ, Castro AJ, Kartje GL. Visual cortical plasticity following unilateral sensorimotor cortical lesions in the neonatal rat. Exp Neurol. 2006;199:122–9.CrossRefPubMed

17.

You Y, Klistorner A, Thie J, Graham SL. Improving reproducibility of VEP recording in rats: electrodes, stimulus source and peak analysis. Doc Ophthalmol. 2011;123:109–19.CrossRefPubMed

18.

Odom JV, Bach M, Brigell M, Holder GE, McCulloch DL, Tormene AP, Vaegan. ISCEV standard for clinical visual evoked potentials (2009 update). Doc Ophthalmol 2010;120:111–119.

19.

Woch G, Aramant RB, Seiler MJ, Sagdullaev BT, McCall MA. Retinal transplants restore visually evoked responses in rats with photoreceptor degeneration. Invest Ophthalmol Vis Sci. 2001;42:1669–76.PubMed

20.

Kanda H, Morimoto T, Fujikado T, Tano Y, Fukuda Y, Sawai H. Electrophysiological studies of the feasibility of suprachoroidal-transretinal stimulation for artificial vision in normal and RCS rats. Invest Ophthalmol Vis Sci. 2004;45:560–6.CrossRefPubMed

21.

Tomita H, Sugano E, Yawo H, Ishizuka T, Isago H, Narikawa S, Kugler S, Tamai M. Restoration of visual response in aged dystrophic RCS rats using AAV-mediated channelopsin-2 gene transfer. Invest Ophthalmol Vis Sci. 2007;48:3821–6.CrossRefPubMed

22.

Huang F, Bladon J, Lagoy RC, Shorrock PN Jr, Hronik-Tupaj M, Zoto CA, Connors RE, McGimpsey WG, Molnar P, Lambert S, Rittenhouse AR, Lambert CR. A photosensitive surface capable of inducing electrophysiological changes in NG108-15 neurons. Acta Biomater. 2015;12:42–50.CrossRefPubMed

Title: Visual evoked potential in RCS rats with Okayama University-type retinal prosthesis (OUReP™) implantation
Authors: Alamusi
Toshihiko Matsuo
Osamu Hosoya
Tetsuya Uchida
Publication date: 01-06-2017
Publisher: Springer Japan
Published in: Journal of Artificial Organs / Issue 2/2017
Print ISSN: 1434-7229
Electronic ISSN: 1619-0904
DOI: https://doi.org/10.1007/s10047-016-0943-4

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Visual evoked potential in RCS rats with Okayama University-type retinal prosthesis (OUReP™) implantation

Abstract

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Please log in to get access to this content

Other articles of this Issue 2/2017

Biventricular support using a centrifugal pump in a 6 year old with fulminant myocarditis

Outcome measures of extracorporeal life support (ECLS) in trauma patients versus patients without trauma: a 7-year single-center retrospective cohort study

Analysis of specific absorption rate and internal electric field in human biological tissues surrounding an air-core coil-type transcutaneous energy transmission transformer

Factors associated with a low initial cerebral oxygen saturation value in patients undergoing cardiac surgery

ECMO for intractable status asthmaticus following atracurium

Proteome analysis of hemofilter adsorbates to identify novel substances of sepsis: a pilot study