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Annals of Vascular Surgery
Published in: Annals of Vascular Surgery 3/2006

01-05-2006 | Basic Science Research

Expansion of Canine Bone Marrow-Derived Endothelial Progenitor Cells and Dynamic Observation

Authors: YingFeng Wu, MD, Jian Zhang, MD, YongQuan Gu, MD, JianXin Li, PhD, Bing Chen, MD, LianRui Guo, MD, Tao Luo, MD, ZhongGao Wang, MD

Published in: Annals of Vascular Surgery | Issue 3/2006

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Abstract

We cultured bone marrow-derived endothelial progenitor cells by a simple ex vivo expansion method and observed the expansion efficacy. Bone marrow mononuclear cells from five mongrel adult dogs were cultured with EGM-2MV medium in culture flasks coated with fibronectin. Morphology was observed with phase contrast microscopy, and a growth curve was constructed to evaluate the efficacy of expansion. Incorporation of Dil-ac-LDL was tested to evaluate the function. At different time points, immunocytochemical staining for flk-1, CD133, and factor VIII-related antigens was done and compared to staining of endothelial cells and mesenchymal stem cells and percentages of CD133, vascular endothelial growth factor receptor 2 (VEGFR-2), and the double-positive cells measured with flow cytometry to identify the quality and efficacy of expansion. Cluster-like attached cells grew to confluence at an average time of 10 days, and the mean number of cells harvested from 1 mL of bone marrow was (1.3 ± 0.3) × 106. The cells presented a cobblestone-like appearance and took up Dil-ac-LDL. Immunocytochemistry showed that flk-1, CD133, and factor VIII-related antigens were positive. Flow cytometry showed that VEGFR-2 and CD133 double-positive cells augmented 242-fold at the tenth day. Ex vivo expansion can effectively proliferate endothelial progenitor cells from bone marrow; the expansion efficacy could meet the requirements for tissue engineering of blood vessels.
Literature
1.
Wang ZG, Du W, Li GD, et al. Rapid cellular luminal coverage of Dacron inferior vena cava prostheses in dogs by immediate seeding of antogenous endothelial cells derived from omental tissue: results of a preliminary trial. J Vasc Surg 1990;12:168–179CrossRefPubMed
2.
Asahara T, Murohara J, Sullivan A, et al. Isolation of putative progenitor endothelial cells for angiogenesis. Science 1997;275:964–967CrossRefPubMed
3.
Peichev M, Naiyer AJ, Pereira D, et al. Expression of VEGFR-2 and AC133 by circulating human CD34+ cells identifies a population of functional endothelial precursors. Blood 2000;95:952–958PubMed
4.
Gehling U, Ergun S, Schumacher U, et al. In vitro differentiation of endothelial cells from AC133 positive progenitor cells. Blood 2000;95:3106–3112PubMed
5.
Quirici N, Soligo D, Caneva L, et al. Differentiation and expansion of endothelial cells from human bone marrow CD133+ cells. Br J Haematol 2001;115:186–194CrossRefPubMed
6.
Boyer M, Townsend LE, Vogel M, et al. Isolation of endothelial cells and their progenitor cells from human peripheral blood. J Vasc Surg 2000;31:181–189CrossRefPubMed
7.
Seifalian AM, Salacinski HJ, Tai NR, et al. A new technique for measuring the cell growth and metabolism of endothelial cells seeded on vascular protheses. J Biomed Mater Res 2001;55:637–644CrossRefPubMed
8.
Kadner A, Hoerstrup SP, Zund G, et al. A new source for cardiovascular tissue engineering: human bone marrow stromal cells. Eur J Cardiothorac Surg 2002;21:1055–1060CrossRefPubMed
9.
Shirota T, He HB, Yasui H, et al. Human endothelial progenitor cell-seeded hybrid graft: proliferative and antithrombogenic potentials in vitro and fabrication processing. Tissue Eng 2003;9:127–135CrossRefPubMed
10.
Shintani S, Murohara T, Ikada H, et al. Augmentation of postnatal neovascularization with autologous bone marrow transplantation. Circulation 2001;103:897–903PubMedCrossRef
11.
Shi Q, Rafii S, Wu MH, et al. Evidence for circulating bone marrow-derived endothelial cells. Blood 1998;92:362–367PubMed
12.
Mihail H, Wolfgang E, Peter CW. Endothelial progenitor cells: mobilization, differentiation, and homing. Arterioscler Thromb Vasc Biol 2003;23:1185–1189CrossRefPubMed
13.
14.
Moehler TM, Neben K, Ho AD, et al. Angiogenesis in hematological malignancies. Ann Hematol 2001;80:695–705CrossRefPubMed
15.
Kawamoto A, Asahara T, Losordo DW. Transplantation of endothelial progenitor cells for therapeutic neovascularization. Cardiovasc Radiat Med 2002;3:221–225CrossRefPubMed
16.
Kalka C, Masuda H, Takahashi T, et al. Transplantation of ex vivo expanded endothelial progenitor cells for therapeutic neovascularization. Proc Natl Acad Sci 2000;97:3422–3427CrossRefPubMed
17.
Stefano RD, Santoni T, Barsotti MC, et al. Different growth conditions for peripheral blood endothelial progenitors. Cardiovasc Radiat Med 2002;3:172–175CrossRefPubMed
18.
Yin AH, Miraglia S, Zanjani ED, et al. AC133, a novel marker for human hematopoietic stem and progenitor cells. Blood 1997;90:5002–5012PubMed
19.
Reyes M, Dudek A, Jahagirdar B, et al. Origin of endothelial progenitors in human postnatal bone marrow. J Clin Invest 2002;109:337–346CrossRefPubMed
20.
Harraz M, Jiao C, Hanlon HD, et al. CD34- blood derived human endothelial cell progenitors. Stem Cells 2001;19:304–312PubMedCrossRef
21.
Griese D, Ehsan A, Melo LG, et al. Isolation and transplantation of autologous circulating endothelial cells into denuded vessels and prothetic grafts. Circulation 2003;108:2710–2715CrossRefPubMed
22.
Wang ZG, Zhang H, Pu LQ, et al. Can endothelial seeding enhance patency and inhibit neointimal hyperplasia? Experimental studies and clinical trial of endothelial seeded venous protheses. Int Angiol 2000;19:259-269PubMed
Metadata
Title
Expansion of Canine Bone Marrow-Derived Endothelial Progenitor Cells and Dynamic Observation
Authors
YingFeng Wu, MD
Jian Zhang, MD
YongQuan Gu, MD
JianXin Li, PhD
Bing Chen, MD
LianRui Guo, MD
Tao Luo, MD
ZhongGao Wang, MD
Publication date
01-05-2006
Publisher
Springer-Verlag
Published in
Annals of Vascular Surgery / Issue 3/2006
Print ISSN: 0890-5096
Electronic ISSN: 1615-5947
DOI
https://doi.org/10.1007/s10016-006-9047-6

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