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Medical Molecular Morphology
Published in: Medical Molecular Morphology 3/2016

01-09-2016 | Original Paper

Intracellular localization of α-tubulin acetyltransferase ATAT1 in rat ciliated cells

Authors: Takashi Nakakura, Takeshi Suzuki, Takahiro Nemoto, Hideyuki Tanaka, Anshin Asano-Hoshino, Kenjiro Arisawa, Yoshimi Nishijima, Yoshiko Kiuchi, Haruo Hagiwara

Published in: Medical Molecular Morphology | Issue 3/2016

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Abstract

Cilia are microtubule-based hair-like organelles on basal bodies located beneath the cell membrane in various tissues of multicellular animals, and are usually classified into motile cilia and primary cilia. Microtubules are assembled from the heterodimers of α- and β-tubulin. The lysine residue at position 40 (K40) of α-tubulin is an important site for acetylation, and this site is acetylated in the cilium. α-Tubulin N-acetyltransferase 1 (ATAT1) is an acetyltransferase specific to the K40 residue of α-tubulin; however, its intracellular distribution in mammalian tissues remains unclear. In this study, we analyzed ATAT1 localization in rat trachea, oviduct, kidney, retina, testis and the third ventricle of the brain by immunohistochemical techniques using a specific antibody against ATAT1. ATAT1 was distributed to the motile cilia of multiciliated cells of the trachea, third ventricle of the brain and oviduct, and in the primary cilia of the renal medullary collecting duct. ATAT1 also localized to the primary cilia, inner and outer segments of retinal photoreceptor cells, and at the Golgi apparatus of spermatocytes and spermatids of testis. These results indicated that α-tubulin acetylation by ATAT1 at distinct subcellular positions may influence the functional regulation of microtubules and cilia in a variety of ciliated cells.
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Metadata
Title
Intracellular localization of α-tubulin acetyltransferase ATAT1 in rat ciliated cells
Authors
Takashi Nakakura
Takeshi Suzuki
Takahiro Nemoto
Hideyuki Tanaka
Anshin Asano-Hoshino
Kenjiro Arisawa
Yoshimi Nishijima
Yoshiko Kiuchi
Haruo Hagiwara
Publication date
01-09-2016
Publisher
Springer Japan
Published in
Medical Molecular Morphology / Issue 3/2016
Print ISSN: 1860-1480
Electronic ISSN: 1860-1499
DOI
https://doi.org/10.1007/s00795-015-0132-1

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