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Published in:

01-05-2021 | Original Article

Biocompatibility, mechanical, and bonding properties of a dental adhesive modified with antibacterial monomer and cross-linker

Authors: Hoda Moussa, Megan M. Jones, Ningbo Huo, Runsheng Zhang, Mayuresh Keskar, Michelle B. Visser, Mark T. Swihart, Chong Cheng, Camila Sabatini

Published in: Clinical Oral Investigations | Issue 5/2021

Abstract

Objectives

This study investigated the antibacterial, cytotoxicity, and mechanical properties of a dental adhesive modified with quaternary ammonium monomer ((2-acryloyloxyethyl)dimethyldodecylammonium bromide) and cross-linker (bis(2-acryloyloxyethyl)methyldodecylammonium bromide).

Materials and methods

Monomer (M), cross-linker (C), or a combination of these (M + C) were incorporated into adhesive Adper Single Bond Plus (SB) in 5, 10, or 25% (as wt%). A colony-forming unit and MTT assays were used to evaluate antibacterial properties against Streptococcus mutans and cell viability. Resin-dentin beams (0.9 ± 0.1 mm²) were evaluated for micro-tensile bond strength (μTBS) after 24 h, 6 months, and 3 years. Hourglass specimens were evaluated for ultimate tensile strength (UTS) after 24 h, 1 week, and 6 months. Micro-hardness measurements after softening in ethanol were taken as an indirect assessment of the polymer cross-linking density. Kruskal-Wallis, one-way ANOVA, two-way ANOVA, and Student’s t test were used for analysis of the antibacterial, cytotoxicity, μTBS, UTS, and hardness data, all with a significance level of p < 0.05.

Results

10%M and 25%M demonstrated a significant reduction in S. mutans relative to SB (p < 0.001). No differences in cytotoxicity were detected for any of the groups. After 6 months, no changes in μTBS were shown for any of the groups. After 3 years, all groups evidenced a significant decrease in μTBS (p < 0.05) except 5%M, 5%C, and 5%M + 5%C. All groups demonstrated either stable or significantly increased UTS after 6 months. Except for the cross-linker groups, a significant decrease in micro-hardness was shown for all groups after softening in ethanol (p < 0.05).

Conclusions

A 5–10% of monomer may render the resin antibacterial without a compromise to its mechanical and bonding properties.

Clinical relevance

Biomodification of a resin adhesive with an antibacterial monomer and cross-linker may help improve the life span of adhesive restorations.

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Title: Biocompatibility, mechanical, and bonding properties of a dental adhesive modified with antibacterial monomer and cross-linker
Authors: Hoda Moussa
Megan M. Jones
Ningbo Huo
Runsheng Zhang
Mayuresh Keskar
Michelle B. Visser
Mark T. Swihart
Chong Cheng
Camila Sabatini
Publication date: 01-05-2021
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 5/2021
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-020-03605-w

At a glance: The STEP trials

Springer Medicine

Biocompatibility, mechanical, and bonding properties of a dental adhesive modified with antibacterial monomer and cross-linker

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The STEP trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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