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01-04-2019 | Original Article

Metallic crown-induced occlusal trauma as a protocol to evaluate inflammatory response in temporomandibular joint and periodontal tissues of rats

Authors: Henrique Ballassini Abdalla, Juliana Trindade Clemente-Napimoga, Ricardo Bonfante, Caio Augusto Hashizume, Wilian Segatto Zanelli, Cristina Gomes de Macedo, Marcelo Henrique Napimoga, Wilkens Aurélio Buarque e Silva, Frederico Andrade e Silva

Published in: Clinical Oral Investigations | Issue 4/2019

Abstract

Objectives

The goal of this study is to propose a standard protocol of experimental occlusal trauma to evaluate the inflammatory hyperalgesia induced by metallic crowns on orofacial tissues of rats.

Materials and methods

Thirty animals were randomly divided into six groups (n = 5 per group). Detailed methodology on the manufacturing of metallic crowns is described. The inflammatory hyperalgesia induced by occlusal interference was evaluated by intra-articular injection of a low dose of 0.5% formalin (30 μl) or vehicle (saline) into temporomandibular joint, 21 or 28 days after metallic crown cementation. Posteriorly, pro-inflammatory cytokines were evaluated by enzyme-linked immunosorbent assay to assess the effect of occlusal interference on periodontium.

Results

The cementation of metallic crowns with dental anatomy on the lower molar of rats does not show signs of stress and lack of feeding. Metallic crown-induced occlusal trauma results in a temporomandibular joint inflammatory hyperalgesia (P < 0.05: ANOVA, Tukey’s test). Otherwise, it was observed that occlusal trauma results in the increase of protein level of pro-inflammatory cytokines TNF-alpha and IL-1beta in the gingival tissues (P < 0.05).

Conclusion

This study demonstrates in detail a methodology of occlusal trauma resulting from the cementation of metallic crowns in the lower molars of rats, mimicking occlusal interferences commonly evaluated in the dental clinic. This methodology makes new studies to better understand the mechanisms involved in the occlusal trauma of orofacial tissues possible.

Clinical relevance

The standardization of an experimental occlusal interference model will allow us to understand the deleterious effect and mechanisms that affect the orofacial tissues.

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Title: Metallic crown-induced occlusal trauma as a protocol to evaluate inflammatory response in temporomandibular joint and periodontal tissues of rats
Authors: Henrique Ballassini Abdalla
Juliana Trindade Clemente-Napimoga
Ricardo Bonfante
Caio Augusto Hashizume
Wilian Segatto Zanelli
Cristina Gomes de Macedo
Marcelo Henrique Napimoga
Wilkens Aurélio Buarque e Silva
Frederico Andrade e Silva
Publication date: 01-04-2019
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 4/2019
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2639-z

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Metallic crown-induced occlusal trauma as a protocol to evaluate inflammatory response in temporomandibular joint and periodontal tissues of rats

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

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