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Clinical Oral Investigations

Published in:

01-02-2019 | Original Article

Effects of two disinfection/sterilization methods for dentin specimens on dentin permeability

Authors: Ruodan Jiang, Yongxiang Xu, Hong Lin

Published in: Clinical Oral Investigations | Issue 2/2019

Abstract

Objectives

To investigate the effects of two disinfection/sterilization methods on the permeability of dentin specimens.

Materials and methods

Forty intact human third molars were freshly extracted and cut, close to the pulp chamber, into dentin disks with a 500-μm thickness. The disks were randomized (n = 20 each) into a 70% ethanol group (acid-etched dentin disks soaked in 70% ethanol for 15 min) and a steam autoclaving group (acid-etched dentin disks autoclaved for 25 min). The permeability (Lp) of each dentin disk was measured before and after either treatment using a hydraulic device, and intra- and inter-group differences in values before and after treatment were analyzed using t tests. Field emission scanning electron microscopy (FE-SEM) micrographs of the dentin surface were acquired and examined. FE-SEM samples were prepared using the critical point drying (CPD) method.

Results

Immersion in 70% ethanol increased the Lp values of dentin specimens by 17%, which was not statistically significant. Steam autoclaving significantly reduced dentin permeability by 66% because the dentin collagen mesh became compact and collapsed, as detected by FE-SEM.

Conclusions

The disinfection of acid-etched dentin disks using 70% ethanol for 15 min does not significantly affect dentin permeability, whereas sterilization of acid-etched dentin disks via autoclaving significantly reduces dentin permeability.

Clinical relevance

Considering the influences of dentin permeability by disinfection/sterilization methods, the disinfection of the acid-etched dentin disks using 70% ethanol for 15 min could be used for the study related to dentin permeability, while the sterilization of autoclaving could not.

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Title: Effects of two disinfection/sterilization methods for dentin specimens on dentin permeability
Authors: Ruodan Jiang
Yongxiang Xu
Hong Lin
Publication date: 01-02-2019
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 2/2019
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2513-z

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Effects of two disinfection/sterilization methods for dentin specimens on dentin permeability

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusions

Clinical relevance

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