Top

Clinical Oral Investigations

Published in:

01-12-2018 | Original Article

Evaluation of the chemopreventive effects of Ankaferd Bloodstopper in 7,12-dimethylbenz[a]anthracene-induced oral epithelial dysplasia

Authors: Murat Ozle, Dilek A. Uğar Çankal, Mert Ilhan, Hikmet Keleş, Esra Küpeli Akkol

Published in: Clinical Oral Investigations | Issue 9/2018

Abstract

Objectives

Ankaferd BloodStopper® (ABS) is an herbal extract which has been used historically as a hemostatic agent in traditional Turkish medicine. ABS comprises of standardized mixture of herbs Thymus vulgaris, Glycyrrhiza glabra, Vitis vinifera, Alpinia officinarum, and Urtica dioica. In addition to its hemostatic effects, the herb ABS contains some other biological effects including antioxidant and antitumoral properties. The aim of this study is to investigate the chemopreventive effects of ABS in 7,12-dimethylbenz[a]anthracene (DMBA)-induced oral epithelial dysplasia.

Materials and methods

A total of 40 Sprague Dawley rats were randomly divided into four groups. Group 1 animals received DMBA alone, and group 2 animals received both DMBA and Ankaferd. Group 3 animals received ABS alone while group 4 animals served as control group and received only liquid paraffine. All animals were sacrificed, and tissue samples were analyzed histologically at the end of the experimental period (14 weeks).

Results

Histological studies have shown that the buccal pouches of animals treated with DMBA alone revealed severe dysplasia while only mild or no dysplasia were noticed in DMBA + ABS group. Ankaferd were administered to animals and control group showed no dysplasia or other oral lesions.

Conclusion

The results suggest that Ankaferd Bloodstopper® has chemopreventive effect against DMBA-induced oral epithelial dysplasia.

Clinical relevance

Ankaferd Bloodstopper® could be used as a supportive treatment option of cancer in oral and maxillofacial surgery since it possesses chemopreventive effect.

Yılmaz M (1999) DMBA (dimetilbenzantrasen) ile dilde meydana getirilen kimyasal kanser modeli üzerinde karsinogenezis sürecinin histopatolojik takibi ve selenyumun karsinogenezis sürecine etksinin incelenmesi. Uzmanlık Tezi, Ankara

Stewart BW, Christopher PW (2014) World Cancer Report 2014. WHO Press

Villa A, Villa C, Abati S (2011) Oral cancer and oral erythroplakia: an update and implication for clinicians. Aust Dent J 56(3):253–256. https://doi.org/10.1111/j.1834-7819.2011.01337.x CrossRefPubMed

Jemal A, Bray F, Center MM, Ferlay J, Ward E, Forman D (2011) Global cancer statistics. CA Cancer J Clin 61(2):69–90CrossRefPubMed

Siegel RL, Miller KD, Jemal A (2018) Cancer statistics, 2018. CA Cancer J Clin 68:7–30CrossRefPubMed

Petersen PE (2009) Oral cancer prevention and control the approach of the World Health Organization. Oral Oncol 45(4–5):454–460. https://doi.org/10.1016/j.oraloncology.2008.05.023 CrossRefPubMed

Ferlay J, Shin HR, Bray F, Forman D, Mathers C, Parkin DM (2010) Estimates of worldwide burden of cancer in 2008: GLOBOCAN 2008. Int J Cancer 127(12):2893–2917. https://doi.org/10.1002/ijc.25516 CrossRefPubMed

Yücetaş Ş (2005) Ağız ve Çevre Dokusu Hastalıkları. Atlas Kitapçılık

Barnes L, Eveson JW, Reichart P, Sidransky D (2005) World Health Organisation classification of tumors. IARC Press, Lyon

10.

Izumo T (2011) Oral premalignant lesions: from the pathological viewpoint. Int J Clin Oncol 16(1):15–26. https://doi.org/10.1007/s10147-010-0169-z CrossRefPubMed

11.

Manoharan S, Vasanthaselvan M, Silvan S, Baskaran N, Kumar Singh A, Vinoth Kumar V (2010) Carnosic acid: a potent chemopreventive agent against oral carcinogenesis. Chem Biol Interact 188(3):616–622. https://doi.org/10.1016/j.cbi.2010.08.009 CrossRefPubMed

12.

Burki HR, Okita GT (1969) Effect of oral copper sulfate on 7,12-dimethylbenz(alpha)anthracene carcinogenesis in mice. Br J Cancer 23(3):591–596CrossRefPubMedPubMedCentral

13.

Salley JJ (1954) Experimental carcinogenesis in the cheek pouch of the Syrian hamster. J Dent Res 33(2):253–262CrossRefPubMed

14.

Fırat HC, Özdemir O, Koşar A, Göker H, Haznedaroğlu İC (2009) Ankaferd Blood Stopper Annual Review of Arkaferd 08-09. Naviga Scientific Publication

15.

Ugur A, Sarac N, Cankal DA, Ozle M (2016) The antioxidant and antimutagenic activities of Ankaferd blood stopper, a natural hemostatic agent used in dentistry. Turk J Med Sci 46:657–663CrossRefPubMed

16.

Dhanarasu S, Selvam M, Salama SM, Shanmugam M, Sethuraman P (2010) Terminalia arjuna (Roxb.) modulates circulatory antioxidants on 7,12-dimethylbenz(a)anthracene- induced hamster buccal pouch carcinogenesis. Oman Med J 25(4):276–281. https://doi.org/10.5001/omj.2010.81 CrossRefPubMedPubMedCentral

17.

Manoharan S, Sindhu G, Vinothkumar V, Kowsalya R (2012) Berberine prevents 7,12-dimethylbenz[a]anthracene-induced hamster buccal pouch carcinogenesis: A biochemical approach. Eur J Cancer Prev 21:182–192CrossRefPubMed

18.

Panjamurthy K, Manoharan S, Nirmal MR, Vellaichamy L (2009) Protective role of Withaferin-A on immunoexpression of p53 and bcl-2 in 7,12-dimethylbenz(a)anthracene-induced experimental oral carcinogenesis. Investig New Drugs 27(5):447–452. https://doi.org/10.1007/s10637-008-9199-z CrossRef

19.

Kuranel E (2012) Plantago lanceolata Bitkisinin Yara İyileştirici Özelliklerinin Araştırılması. Yüksek Lisans, İnönü Üniversitesi, Malatya

20.

Sedlak J, Lindsay RH (1968) Estimation of total, protein-bound, and nonprotein sulfhydryl groups in tissue with Ellman’s reagent. Anal Biochem 25(1):192–205CrossRefPubMed

21.

Ye X, Krohn RL, Liu W, Joshi SS, Kuszynski CA, McGinn TR, Bagchi M, Preuss HG, Stohs SJ, Bagchi D (1999) The cytotoxic effects of a novel IH636 grape seed proanthocyanidin extract on cultured human cancer cells. Mol Cell Biochem 196(1–2):99–108CrossRefPubMed

22.

Giovannelli L, Innocenti M, Santamaria AR, Bigagli E, Pasqua G, Mulinacci N (2014) Antitumoural activity of viniferin-enriched extracts from Vitis vinifera L. cell cultures. Nat Prod Rep 28(22):2006–2016. https://doi.org/10.1080/14786419.2014.924935 CrossRef

23.

Aghbali A, Hosseini SV, Delazar A, Gharavi NK, Shahneh FZ, Orangi M, Bandehagh A, Baradaran B (2013) Induction of apoptosis by grape seed extract (Vitis vinifera) in oral squamous cell carcinoma. Bosn J Basic Med Sci 13(3):186–191CrossRefPubMedPubMedCentral

24.

Niture SK, Rao US, Srivenugopal KS (2006) Chemopreventative strategies targeting the MGMT repair protein: Augmented expression in human lymphocytes and tumor cells by ethanolic and aqueous extracts of several Indian medicinal plants. Int J Oncol 29(5):1269–1278PubMed

25.

Clifford AJ, Ebeler SE, Ebeler JD, Bills ND, Hinrichs SH, Teissedre PL, Waterhouse AL (1996) Delayed tumor onset in transgenic mice fed an amino acid-based diet supplemented with red wine solids. Am J Clin Nutr 64(5):748–756CrossRefPubMed

26.

Jung KJ, Wallig MA, Singletary KW (2006) Purple grape juice inhibits 7,12-dimethylbenz[a]anthracene (DMBA)-induced rat mammary tumorigenesis and in vivo DMBA-DNA adduct formation. Cancer Lett 233(2):279–288. https://doi.org/10.1016/j.canlet.2005.03.020 CrossRefPubMed

27.

Kim H, Hall P, Smith M, Kirk M, Prasain JK, Barnes S, Grubbs C (2004) Chemoprevention by grape seed extract and genistein in carcinogen-induced mammary cancer in rats is diet dependent. J Nutr 134(12):3445–3452CrossRef

28.

Morre DM, Morre DJ (2006) Anticancer activity of grape and grape skin extracts alone and combined with green tea infusions. Cancer Lett 238(2):202–209. https://doi.org/10.1016/j.canlet.2005.07.011 CrossRefPubMed

29.

Soleas GJ, Grass L, Josephy PD, Goldberg DM, Diamandis EP (2002) A comparison of the anticarcinogenic properties of four red wine polyphenols. Clin Biochem 35(2):119–124CrossRefPubMed

30.

Aggarwal BB, Bhardwaj A, Aggarwal RS, Seeram NP, Shishodia S, Takada Y (2004) Role of resveratrol in prevention and therapy of cancer: Preclinical and clinical studies. Anticancer Res 24(5A):2783–2840PubMed

31.

Ebeler SE, Brenneman CA, Kim GS, Jewell WT, Webb MR, Chacon-Rodriguez L, MacDonald EA, Cramer AC, Levi A, Ebeler JD, Islas-Trejo A, Kraus A, Hinrichs SH, Clifford AJ (2002) Dietary catechin delays tumor onset in a transgenic mouse model. Am J Clin Nutr 76(4):865–872CrossRefPubMed

32.

Garvin S, Ollinger K, Dabrosin C (2006) Resveratrol induces apoptosis and inhibits angiogenesis in human breast cancer xenografts in vivo. Cancer Lett 231(1):113–122. https://doi.org/10.1016/j.canlet.2005.01.031 CrossRefPubMed

33.

Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG, Heber D (2005) In vitro antiproliferative, apoptotic and antioxidant activities of punicalagin, ellagic acid and a total pomegranate tannin extract are enhanced in combination with other polyphenols as found in pomegranate juice. J Nutr Biochem 16(6):360–367CrossRefPubMed

34.

Klinge CM, Risinger KE, Watts MB, Beck V, Eder R, Jungbauer A (2003) Estrogenic activity in white and red wine extracts. J Agric Food Chem 51(7):1850–1857. https://doi.org/10.1021/jf0259821 CrossRefPubMed

35.

Schlachterman A, Valle F, Wall KM, Azios NG, Castillo L, Morell L, Washington AV, Cubano LA, Dharmawardhane SF (2008) Combined resveratrol, quercetin, and catechin treatment reduces breast tumor growth in a nude mouse model. Transl Oncol 1(1):19–27CrossRefPubMedPubMedCentral

36.

Schmitt E, Stopper H (2001) Estrogenic activity of naturally occurring anthocyanidins. Nutr Cancer 41(1–2):145–149. https://doi.org/10.1080/01635581.2001.9680625 CrossRefPubMed

37.

Sun Q, Prasad R, Rosenthal E, Katiyar SK (2011) Grape seed proanthocyanidins inhibit the invasive potential of head and neck cutaneous squamous cell carcinoma cells by targeting EGFR expression and epithelial-to-mesenchymal transition. BMC Complement Altern Med 11:134. https://doi.org/10.1186/1472-6882-11-134 CrossRefPubMedPubMedCentral

Title: Evaluation of the chemopreventive effects of Ankaferd Bloodstopper in 7,12-dimethylbenz[a]anthracene-induced oral epithelial dysplasia
Authors: Murat Ozle
Dilek A. Uğar Çankal
Mert Ilhan
Hikmet Keleş
Esra Küpeli Akkol
Publication date: 01-12-2018
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 9/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2399-9

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Evaluation of the chemopreventive effects of Ankaferd Bloodstopper in 7,12-dimethylbenz[a]anthracene-induced oral epithelial dysplasia

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

At a glance: The ONWARDS insulin icodec trials

Springer Medicine

Abstract

Objectives

Materials and methods

Results

Conclusion

Clinical relevance

Please log in to get access to this content

Other articles of this Issue 9/2018

Assessing abrasion of orthodontic surface sealants using a modified ophthalmic optical coherence tomography device

Allogeneic bone block for challenging augmentation—a clinical, histological, and histomorphometrical investigation of tissue reaction and new bone formation

Dentinal tubule obliteration using toothpastes containing sodium trimetaphosphate microparticles or nanoparticles

Effect of multiple autoclave cycles on the surface roughness of HyFlex CM and HyFlex EDM files: an atomic force microscopy study

Bone mineralization and vascularization in bisphosphonate-related osteonecrosis of the jaw: an experimental study in the rat

Reduced salivary amylase activity in metabolic syndrome patients with obesity could be improved by treatment with a dipeptidyl peptidase IV inhibitor