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Clinical Oral Investigations

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01-12-2018 | Original Article

Effect of multiple autoclave cycles on the surface roughness of HyFlex CM and HyFlex EDM files: an atomic force microscopy study

Authors: K. Yılmaz, G. Uslu, T. Özyürek

Published in: Clinical Oral Investigations | Issue 9/2018

Abstract

Aim

To compare the effect of autoclave cycles on the surface topography and roughness of HyFlex CM and HyFlex EDM instruments using atomic force microscopy (AFM) analysis.

Methodology

Eight new files of each brand were subdivided into four subgroups (n = 2/each subgroup). One group was allocated as the control group and not subjected to autoclave sterilization. The other three groups were subjected to different numbers (1, 5, and 10) of autoclave sterilization cycles. After the cycle instruments were subjected to AFM analysis. Roughness average (Ra) and the root mean square (RMS) values were chosen to investigate the surface features of endodontic files. The data was analyzed using one-way ANOVA and post hoc Tamhane tests at 5% significant level.

Results

The lowest Ra and RMS values were observed in the HyFlex EDM files that served as the control and in those subjected to a single cycle of autoclave sterilization (P < 0.05). The highest Ra and RMS values were observed in the HyFlex CM and HyFlex EDM files that were subjected to 10 cycles of autoclave sterilization (P < 0.05). The surface roughness values of the HyFlex CM group showed a significant increase after ten autoclave cycles, whereas those of the HyFlex EDM group exhibited a significant change after five autoclave cycles (P < 0.05).

Conclusions

Although the initial surface roughness values of the HyFlex EDM files were lower than those of the HyFlex CM files, the surface roughness values of the EDM files showed a statistically significant increase after 5 cycles of autoclave sterilization. In contrast, the surface roughness values of the HyFlex CM files did not increase until 10 cycles of autoclave sterilization.

Clinical relevance

Present study indicated that autoclave sterilization negatively affected the surface roughness of the tested NiTi files.

Thompson SA (2000) An overview of nickel–titanium alloys used in dentistry. Int Endod J 33:297–310CrossRef

Peters OA (2000) Current challenges and concepts in the preparation of root canal systems: a review. J Endod 30:559–567CrossRef

Gambarini G, Plotino G, Grande NM, Al-Sudani D, De Luca M, Testarelli L (2011) Mechanical properties of nickel–titanium rotary instruments produced with a new manufacturing technique. Int Endod J 44:337–341CrossRef

Shen Y, Zhou HM, Zheng YF, Peng B, Haapasalo M (2013) Current challenges and concepts of the thermomechanical treatment of nickel-titanium instruments. J Endod 39:163–172CrossRef

Braga LCM, Silva ACF, Buono VTL, de Azevedo Bahia MG (2014) Impact of heat treatments on the fatigue resistance of different rotary nickel-titanium instruments. J Endod 40:1494–1497CrossRef

Plotino G, Testarelli L, Al-Sudani D, Pongione G, Grande NM, Gambarini G (2014) Fatigue resistance of rotary instruments manufactured using different nickel-titanium alloys: a comparative study. Odontology 102:31–35CrossRef

Iacono F, Pirani C, Generali L et al (2016) Structural analysis of HyFlex EDM instruments. Int Endod J 50:303–313CrossRef

Abbas NM, Solomon DG, Bahari MF (2007) A review on current research trends in electrical discharge machining (EDM). Int J Mach Tool Manu 47:1214–1228CrossRef

Daneshmand S, Kahrizi EF, Abedi E, Abdolhosseini MM (2013) Influence of machining parameters on electro discharge machining of NiTi shape memory alloys. Int J Electrochem Sci 8:3095–3104

10.

Pirani C, Iacono F, Generali L, Sassatelli P, Nucci C, Lusvarghi L, Gandolfi MG, Prati C (2016) HyFlex EDM: superficial features, metallurgical analysis and fatigue resistance of innovative electro discharge machined NiTi rotary instruments. Int Endod J 49:483–493CrossRef

11.

Spagnuolo G, Ametrano G, D'antò V et al (2012) Effect of autoclaving on the surfaces of TiN-coated and conventional nickel–titanium rotary instruments. Int Endod J 45:1148–1155CrossRef

12.

Zhao D, Shen Y, Peng B, Haapasalo M (2015) Effect of autoclave sterilization on the cyclic fatigue resistance of thermally treated nickel–titanium instruments. Int Endod J 49:990–995CrossRef

13.

Casper RB, Roberts HW, Roberts MD, Himel VT, Bergeron BE (2011) Comparison of autoclaving effects on torsional deformation and fracture resistance of three innovative endodontic file systems. J Endod 37:1572–1575CrossRef

14.

Valois CR, Silva LP, Azevedo RB (2008) Multiple autoclave cycles affect the surface of rotary nickel-titanium files: an atomic force microscopy study. J Endod 34:859–862CrossRef

15.

Alapati SB, Brantley WA, Svec TA, Powers JM, Nusstein JM, Daehn GS (2005) SEM observations of nickel-titanium rotary endodontic instruments that fractured during clinical use. J Endod 31:40–43CrossRef

16.

Valois CR, Silva LP, Azevedo RB (2005) Atomic force microscopy study of stainless-steel and nickel-titanium files. J Endod 31:882–885CrossRef

17.

Topuz O, Aydin C, Uzun O, Inan U, Alacam T, Tunca YM (2008) Structural effects of sodium hypochlorite solution on RaCe rotary nickel-titanium instruments: an atomic force microscopy study. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 105:661–665CrossRef

18.

Fayyad DM, Mahran AH (2014) Atomic force microscopic evaluation of nanostructure alterations of rotary NiTi instruments after immersion in irrigating solutions. Int Endod J 47:567–573CrossRef

19.

Prasad PS, Sam JE, Arvind Kumar K (2014) The effect of 5% sodium hypochlorite, 17% EDTA and triphala on two different rotary Ni-Ti instruments: an AFM and EDS analysis. J Conserv Dent 17:462–466CrossRef

20.

Plotino G, Costanzo A, Grande NM, Petrovic R, Testarelli L, Gambarini G (2012) Experimental evaluation on the influence of autoclave sterilization on the cyclic fatigue of new nickel-titanium rotary instruments. J Endod 38:222–225CrossRef

21.

ColteneEndo HyFlex EDM Brochure. Available at: https://www.coltene.com/fileadmin/Data/EN/Products/Endodontics/Root_Canal_Shaping/HyFlex_EDM/31328A_HyFlexEDM_Brochure_US.pdf. Accessed 15 June 2017

22.

ColteneEndo HyFlex CM Brochure. Available at: http://www.hyflexcm.com/DevDownloads/HyFlexFamily_bro.pdf. Accessed 15 June 2017

23.

Peters OA, Gluskin AK, Weiss RA, Han JT (2012) An in vitro assessment of the physical properties of novel Hyflex nickel–titanium rotary instruments. Int Endod J 45:1027–1034CrossRef

24.

Shen Y, Coil JM, Zhou H, Zheng Y, Haapasalo M (2013) HyFlex nickel–titanium rotary instruments after clinical use: metallurgical properties. Int Endod J 46:720–729CrossRef

25.

Alfoqom Alazemi M, Bryant ST, Dummer PMH (2015) Deformation of HyFlex CM instruments and their shape recovery following heat sterilization. Int Endod J 48:593–601CrossRef

26.

Alexandrou G, Chrissafis K, Vasiliadis L, Pavlidou E, Polychroniadis EK (2006) Effect of heat sterilization on surface characteristics and microstructure of Mani NRT rotary nickel–titanium instruments. Int Endod J 39:770–778CrossRef

27.

Nair AS, Tilakchand M, Naik BD (2015) The effect of multiple autoclave cycles on the surface of rotary nickel-titanium endodontic files: an in vitro atomic force microscopy investigation. J Conserv Dent 18:218–222CrossRef

28.

Cazaux J (2005) Recent developments and new strategies in scanning electron microscopy. J Microsc 217:16–35CrossRef

29.

Fatma Y, Ozgur U (2014) Evaluation of surface topography changes in three NiTi file systems using rotary and reciprocal motion: an atomic force microscopy study. Microsc Res Tech 77:177–182CrossRef

30.

Cai JJ, Tang XN, Ge JY (2017) Effect of irrigation on surface roughness and fatigue resistance of controlled memory wire nickel-titanium instruments. Int Endod J 50:718–724CrossRef

31.

Iacono F, Pirani C, Generali L, Sassatelli P, Nucci C, Gandolfi MG, Prati C (2016) Wear analysis and cyclic fatigue resistance of electro discharge machined NiTi rotary instruments. G Ital Endod 30:64–68

32.

Eggert C, Peters O, Barbakow F (1999) Wear of nickel-titanium Lightspeed instruments evaluated by scanning electron microscopy. J Endod 25:494–497CrossRef

33.

Yokoyama KI, Kaneko K, Yabuta E, Asaoka K, Sakai JI (2004) Fracture of nickel–titanium superelastic alloy in sodium hypochlorite solution. Mater Sci Eng 369:43–48CrossRef

34.

Seago ST, Bergeron BE, Kirkpatrick TC, Roberts MD, Roberts HW, Himel VT, Sabey KA (2015) Effect of repeated simulated clinical use and sterilization on the cutting efficiency and flexibility of Hyflex CM nickel-titanium rotary files. J Endod 41:725–728CrossRef

Title: Effect of multiple autoclave cycles on the surface roughness of HyFlex CM and HyFlex EDM files: an atomic force microscopy study
Authors: K. Yılmaz
G. Uslu
T. Özyürek
Publication date: 01-12-2018
Publisher: Springer Berlin Heidelberg
Published in: Clinical Oral Investigations / Issue 9/2018
Print ISSN: 1432-6981
Electronic ISSN: 1436-3771
DOI: https://doi.org/10.1007/s00784-018-2382-5

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Effect of multiple autoclave cycles on the surface roughness of HyFlex CM and HyFlex EDM files: an atomic force microscopy study

Abstract

Aim

Methodology

Results

Conclusions

Clinical relevance

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Abstract

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Conclusions

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